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G-Sep™ 25

G-Sep™ is a gel filtration resin comprised of ultrapure cross-linked dextran for desalting and buffer exchange in industrial applications. It exhibits high selectivity, superb resolution, low non-specific adsorption and robust chemical stability.
 
  • Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  • Excellent recovery and minimum sample dilution
  • Available in prepacked SpinOUT™ Desalting columns for fast and convenient desalting
 


Molecules purified with G-Sep™ are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules.
 


Two different G-Sep™ resins are available, G-Sep™ 25 and G-Sep™ 50. They differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. G-Sep™ 25 is better suited for smaller molecules and G-Sep™ 50 is better suited for larger molecules. G-Sep™ 25 and G-Sep™ 50 are both available in 3 different particle sizes (Superfine, Fine, and Medium).
 


The molecular weight cut-off (MWCO) for G-Sep™ 25 is 5 kD for proteins and 10 bases for nucleic acids. The molecular weight cut-off (MWCO) for G-Sep™ 50 is 25 kD for proteins and 20 bases for nucleic acids.
 


Buffer and pH effects on resolution are minimal and purified biomolecules are not significantly diluted (1.5-fold) when processed using G-Sep™.
 


G-Sep™ is autoclavable at 121°C, pH 7 for 30 minutes and is stable in all commonly used buffers, including: 0.2M NaOH; 0.2M HCl; 1M acetic acid; 8M urea; 6M guanidine HCI; 1% SOS, 24% Ethanol; 30% Propanol; and 30% Acetonitrile.
 




Features 
 
G-Sep™ 25 Superfine Fine Medium
BioProcess resin Yes Yes Yes
Matrix Cross-linked dextran Cross-linked dextran Cross-linked dextran
Wet bead size 35–90 µm 35-140 μm 38–235 µm
Dry bead size 20-50 μm 20-80 μm 50-150 μm
Water regain 2.15 – 2.25 mL/g 2.15 – 2.25 mL/g 2.15 – 2.25 mL/g
Swelling 4 – 6 mL/g 4 – 6 mL/g 4 – 6 mL/g
MWCO (size exclusion) below 5000 Da below 5000 Da below 5000 Da
Fractionation range Mr Globular proteins 1000 - 5000 Da 1000 - 5000 Da 1000 - 5000 Da
pH Stability 2.0 to 13.0 2.0 to 13.0 2.0 to 13.0
Pressure Flow Specification >11 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.  >47 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.  >100 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d. 
  • Storage: 4 to 30°C, dry resins. Used resins 4 to 8 °C in 20% Ethanol or 0.1 M NaOH 
  • Chemical Stability: Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile 

Applications

  • Protein purification and contamination removal from samples
  • For the desalting and buffer exchange of protein and nucleic acid solutions
  • Separate proteins from peptides
  • Desalt peptides 
Protocol
786-1390
786-1391
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786-1397
786-1398
Material Safety Data Sheet
786-1390
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786-1391
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786-1392
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786-1393
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786-1394
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786-1395
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786-1396
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786-1397
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786-1398
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G-Sep™ 25
G-Sep™ 25
G-Sep™ is a gel filtration resin comprised of ultrapure cross-linked dextran for desalting and buffer exchange in industrial applications. It ex..
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