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G-Sep™ 50

G-Sep™ is a gel filtration resin comprised of ultrapure cross-linked dextran for desalting and buffer exchange in industrial applications. It exhibits high selectivity, superb resolution, low non-specific adsorption and robust chemical stability.
  • Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  • Excellent recovery and minimum sample dilution
  • Available in prepacked SpinOUT™ Desalting columns for fast and convenient desalting
     
Molecules purified with G-Sep™ are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules.

Two different G-Sep™ resins are available, G-Sep™ 25 and G-Sep™ 50. They differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. G-Sep™ 25 is better suited for smaller molecules and G-Sep™ 50 is better suited for larger molecules. G-Sep™ 25 and G-Sep™ 50 are both available in 3 different particle sizes (Superfine, Fine, and Medium).
 
The molecular weight cut-off (MWCO) for G-Sep™ 25 is 5 kD for proteins and 10 bases for nucleic acids. The molecular weight cut-off (MWCO) for G-Sep™ 50 is 25 kD for proteins and 20 bases for nucleic acids.
 
Buffer and pH effects on resolution are minimal and purified biomolecules are not significantly diluted (1.5-fold) when processed using G-Sep™.
 
G-Sep™ is autoclavable at 121°C, pH 7 for 30 minutes and is stable in all commonly used buffers, including: 0.2M NaOH; 0.2M HCl; 1M acetic acid; 8M urea; 6M guanidine HCI; 1% SOS, 24% Ethanol; 30% Propanol; and 30% Acetonitrile.
 

Features
G-Sep™ 50 Superfine Fine Medium
BioProcess resin Yes Yes Yes
Matrix Cross-linked dextran Cross-linked dextran Cross-linked dextran
Wet bead size 40–100 µm 40-160 µm 100-300 µm
Dry bead size 20–50 µm 20–80 µm 50-150 µm
Water regain 4.80-5.20 mL/g 4.80-5.20 mL/g 4.80-5.20 mL/g
Swelling 9-11 mL/g 9-11 mL/g 9-11 mL/g
MWCO (size exclusion) below 25,000 Da below 25,000 Da below 25,000 Da
Fractionation range Mr Globular proteins 1,000-30,000 Da 1,000-30,000 Da 1,000-30,000 Da
Fractionantion Mp Dextrans 500-10,000 Da 500-10,000 Da 500-10,000 Da
pH Stability  2.0-13.0 2.0-13.0 2.0-13.0
Pressure Flow Specification min 60 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, column 5 cm i.d. min 150 cm/h, bed height 10 cm, column 5 cm i.d.  min 150 cm/h, bed height 10 cm, column 5 cm i.d. 
  • 4 to 30°C, dry resins. Used resins 4 to 8 °C in 20% Ethanol or 0.1 M NaOH
  • Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile 
Applications
  • Protein purification and contamination removal from samples
  • For the desalting and buffer exchange of protein and nucleic acid solutions
  • Separate proteins from peptides
  • Desalt peptides 

 
Protocol
786-1399
786-1400
786-1401
786-1402
786-1403
786-1404
786-1405
786-1406
Material Safety Data Sheet
786-1399
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786-1400
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786-1401
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786-1402
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786-1403
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786-1404
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786-1405
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786-1406
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G-Sep™ 50
G-Sep™ 50
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