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SpinOUT™ G-Acryl

The SpinOUT™ G-Acryl columns are versatile, spin-format columns for the desalting and buffer exchange of peptide and protein and other macromolecule solutions ranging from 5µl through to 3ml sample volumes.  The SpinOUT™ columns are available in three MWCO sizes for >1,800, >6,000 or >20,000 dalton peptides or proteins and are suitable for samples containing as little as 20µg peptide or protein/ml. Also available in 96-well spin plate formats for processing up to 96 samples.
 
The SpinOUT™ G-Acryl columns consist of porous polyacrylamide beads that are extremely hydrophilic and essentially free of charge. The resin is compatible with the following reagents:
  • Dilute Organic Acids
  • Urea [8M]
  • Guanidine-HCl [6M]
  • Chaotropic Agents
  • Reducing Agents (DTT, 2-mercaptoethanol)
  • Detergents (SDS, CHAPS, Triton® X-100, etc)
  • Alcohols (<20%)
  • Formamide

The SpinOUT™ G-Acryl columns are simply to use as the peptide or protein solution is applied and then centrifuged to recover protein with the column retaining >95% of the salts and small molecules.

Five sizes are available for each MWCO and are highlighted below:
 
Cat. # Description # Supplied Resin Bed Volume (ml) Sample Load Volume (ml)
786-1609 SpinOUT™ G-Acryl 100, 0.1ml 25 0.1 0.005-0.03
786-1610 SpinOUT™ G-Acryl 100, 1ml 10 1 0.03-0.3
786-1611
786-1612		 SpinOUT™ G-Acryl 100, 3ml 10
50		 3 0.3-0.9
786-1613
786-1614		 SpinOUT™ G-Acryl 100, 5ml 5
50		 5 0.75-1.5
786-1615 SpinOUT™ G-Acryl 100, 10ml 5 10 1-3
786-1618 SpinOUT™ G-Acryl 600, 0.1ml 25 0.1 0.005-0.03
786-1619
786-1620		 SpinOUT™ G-Acryl 600, 1ml 10
50		 1 0.03-0.3
786-1621
786-1622		 SpinOUT™ G-Acryl 600, 3ml 10
50		 3 0.3-0.9
786-1623
786-1624		 SpinOUT™ G-Acryl 600, 5ml 5
50		 5 0.75-1.5
786-1625 SpinOUT™ G-Acryl 600, 10ml 5 10 1-3
786-1626
786-1627		 SpinOUT™ G-Acryl 600, Spin Plate 2 x 96 well
4 x 96 well		 0.1 0.005-0.03
786-1630 SpinOUT™ G-Acryl 1200, 0.1ml 25 0.1 0.005-0.03
786-1631 SpinOUT™ G-Acryl 1200, 1ml 10 1 0.03-0.3
786-1632 SpinOUT™ G-Acryl 1200, 3ml 10 3 0.3-0.9
786-1633 SpinOUT™ G-Acryl 1200, 5ml 5 5 0.75-1.5
786-1634 SpinOUT™ G-Acryl 1200, 10ml 5 10 1-3
786-1637
786-1638		 SpinOUT™ G-Acryl 1200, Spin Plate 2 x 96 well
4 x 96 well		 0.1 0.005-0.03

Also available in 96-well spin plate formats for processing up to 96 samples. 

Features

  • Polyacrylamide beads for low protein binding
  • Offered in 3 MWCO sizes: >1,800Da,  >6kDa and >20kDa proteins 
  • Available in 5 sizes for sample volumes of 5µl to 3ml
  • 96-well plate formats available
  • Easy-to-use, spin-format columns for rapid purification

Applications

  • Protein purification and contamination removal from samples
  • For the desalting and buffer exchange of protein and nucleic acid solutions
  • Separate proteins from peptides
  • Desalt peptides 
Protocol
786-1609
786-1610
786-1611
786-1612
786-1613
786-1614
786-1615
786-1618
786-1619
786-1620
786-1621
786-1622
786-1623
786-1624
786-1625
786-1626
786-1627
786-1630
786-1631
786-1632
786-1633
786-1634
786-1635
786-1636
Material Safety Data Sheet
786-1609
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786-1610
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786-1611
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786-1612
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786-1613
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786-1614
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786-1615
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786-1618
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786-1619
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786-1620
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786-1621
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786-1622
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786-1623
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786-1624
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786-1625
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786-1626
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786-1627
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786-1630
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786-1631
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786-1632
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786-1633
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786-1634
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786-1635
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786-1636
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SpinOUT™ G-Acryl
SpinOUT™ G-Acryl
The SpinOUT™ G-Acryl columns are versatile, spin-format columns for the desalting and buffer exchange of peptide and protein and other macromole..
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