The SpinOUT™ G-Acryl columns are versatile, spin-format columns for the desalting and buffer exchange of peptide and protein and other macromolecule solutions ranging from 5µl through to 3ml sample volumes. The SpinOUT™ columns are available in three MWCO sizes for >1,800, >6,000 or >20,000 dalton peptides or proteins and are suitable for samples containing as little as 20µg peptide or protein/ml. Also available in 96-well spin plate formats for processing up to 96 samples.
The SpinOUT™ G-Acryl columns consist of porous polyacrylamide beads that are extremely hydrophilic and essentially free of charge. The resin is compatible with the following reagents:
- Dilute Organic Acids
- Urea [8M]
- Guanidine-HCl [6M]
- Chaotropic Agents
- Reducing Agents (DTT, 2-mercaptoethanol)
- Detergents (SDS, CHAPS, Triton® X-100, etc)
- Alcohols (<20%)
- Formamide
The SpinOUT™ G-Acryl columns are simply to use as the peptide or protein solution is applied and then centrifuged to recover protein with the column retaining >95% of the salts and small molecules.
Five sizes are available for each MWCO and are highlighted below:
Cat. # |
Description |
# Supplied |
Resin Bed Volume (ml) |
Sample Load Volume (ml) |
786-1609 |
SpinOUT™ G-Acryl 100, 0.1ml |
25 |
0.1 |
0.005-0.03 |
786-1610 |
SpinOUT™ G-Acryl 100, 1ml |
10 |
1 |
0.03-0.3 |
786-1611
786-1612 |
SpinOUT™ G-Acryl 100, 3ml |
10
50 |
3 |
0.3-0.9 |
786-1613
786-1614 |
SpinOUT™ G-Acryl 100, 5ml |
5
50 |
5 |
0.75-1.5 |
786-1615 |
SpinOUT™ G-Acryl 100, 10ml |
5 |
10 |
1-3 |
786-1618 |
SpinOUT™ G-Acryl 600, 0.1ml |
25 |
0.1 |
0.005-0.03 |
786-1619
786-1620 |
SpinOUT™ G-Acryl 600, 1ml |
10
50 |
1 |
0.03-0.3 |
786-1621
786-1622 |
SpinOUT™ G-Acryl 600, 3ml |
10
50 |
3 |
0.3-0.9 |
786-1623
786-1624 |
SpinOUT™ G-Acryl 600, 5ml |
5
50 |
5 |
0.75-1.5 |
786-1625 |
SpinOUT™ G-Acryl 600, 10ml |
5 |
10 |
1-3 |
786-1626
786-1627 |
SpinOUT™ G-Acryl 600, Spin Plate |
2 x 96 well
4 x 96 well |
0.1 |
0.005-0.03 |
786-1630 |
SpinOUT™ G-Acryl 1200, 0.1ml |
25 |
0.1 |
0.005-0.03 |
786-1631 |
SpinOUT™ G-Acryl 1200, 1ml |
10 |
1 |
0.03-0.3 |
786-1632 |
SpinOUT™ G-Acryl 1200, 3ml |
10 |
3 |
0.3-0.9 |
786-1633 |
SpinOUT™ G-Acryl 1200, 5ml |
5 |
5 |
0.75-1.5 |
786-1634 |
SpinOUT™ G-Acryl 1200, 10ml |
5 |
10 |
1-3 |
786-1637
786-1638 |
SpinOUT™ G-Acryl 1200, Spin Plate |
2 x 96 well
4 x 96 well |
0.1 |
0.005-0.03 |
Also available in 96-well spin plate formats for processing up to 96 samples.
Features
- Polyacrylamide beads for low protein binding
- Offered in 3 MWCO sizes: >1,800Da, >6kDa and >20kDa proteins
- Available in 5 sizes for sample volumes of 5µl to 3ml
- 96-well plate formats available
- Easy-to-use, spin-format columns for rapid purification
Applications
- Protein purification and contamination removal from samples
- For the desalting and buffer exchange of protein and nucleic acid solutions
- Separate proteins from peptides
- Desalt peptides