G-Sep™ Butyl Agarose Fast Flow

G-Sep™ Butyl Agarose Fast Flow (FF) is a 6% cross-linked agarose with butyl groups for Hydrophobic Interaction Chromatography (HIC).  The resin is used to separate biomolecules on the basis of relative hydrophobicity.  

G-Sep™ Agaroses for HIC are also available with octyl and phenyl groups immobilized on 6% cross-linked agarose beads through neutral and highly stable ether linkages.

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Features

• Matrix: Highly cross-linked Agarose, 6%
• Bead form: Spherical, diameter 50-160μm
• Ligand: Butyl
• Ligand Concentration: About 40μmol /ml
• Binding Capacity: About 20mg HSA/ml resin
• pH stability Working Range: 3-13
• pH stability Cleaning-in-Place (CIP): 2-14
• Maximum Pressure (MPa): 0.3
• Maximum Flow Velocity: 450cm/h
• Exclusion Limit (Globular Proteins): 4 x 10⁶
• Physical Stability: Negligible volume variation due to changes in pH or ionic strength
• Chemical Stability: Stable to all commonly used aqueous buffers: 1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol
• Sterilization: Autoclavable, In 1M NaOH,121 °C, pH 7, for 30 min
• Storage Conditions: 4 to 30°C, 20% Ethanol

Applications

• Hydrophobic Interaction Chromatography