Classic Immunoprecipitation

Classic Immunoprecipitation

Immunoprecipitation (IP) is one of the most useful immunochemical techniques.  IPs are routinely used to determine  the presence and quantity of an antigen, molecular weight of a polypeptide, rate of synthesis or degradation, identify certain post translational modifications and interactions with other proteins, nucleic acids and ligands.  IPs consist of four main steps:

1. Labeling of the antigen (Optional step)
2. Release of antigen by cell lysis
3. Formation of antibody-antigen complexes
4. Purification of the immune complexes.
 
G-Biosciences Classic Immunoprecipitation kit contains all the reagents necessary to complete all aspects of immunoprecipitation, with the exception of labeling.  The kit is supplied with Immobilized Protein A/G resin, spin columns and buffers.  The kit is designed for effective immunoprecipitations using <10µg antibody.  The kit is suitable for 50 reactions using 10µl Immobilized Protein A/G Agarose.
 


Features

  • Rapid immunoprecipitations
  • Mild elution conditions
  • Suitable for most common immunoglobulins


Applications

  • For traditional immunoprecipitations

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PROTOCOL
786-637
SDS
786-637
Other Technical Literatures Description
Immunoprecipitation Technical Guide
Catalog Description Size Price(USD) Qty
786-637
Classical Immunoprecipitation Kit 50 Reactions
$256.00