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Purification & Chromatography

G-Biosciences offers protein purification and protein chromatography reagents, including agarose resins for coupling researchers ligands, via versus coupling chemistries, or pre-coupled ligands for purification of specific protein tags. We offer activated agarose resins for conjugation of proteins and biomolecules through amines, carbohydrates, carboxyls, sulfhydryls, hydroxyls and activated hydrogens for coupling your ligand to agarose or magnetic beads. We offer premade protein purification resins for the isolation of specific protein groups or protein tags, including streptavidin and avidin purification, biotinylated protein purification, calmodulin binding protein (CBP) purification, C-Reactive protein purification, GST tagged protein purification, 6X His tagged protein purification, Heparin binding protein purificaiton and glycoprotein purification with immobilized lectins. For antibody purification resins, Protein A resin, Protein G resin and Protein A/G resin are offered in multiple formats, including resin, spin columns and FPLC columns.  Several antibody purification products based on our proprietary Pearl™ IgG Purification resin are offered, including an antibody clean-up kit and a monoclonal antibody purification kit.

Gel filtration resins or size exclusion resins are also offered as resin alone or as spin format columns. The resins offered are G-Sep™-25 and G-Sep™-50 that are equivalents to the well established gel filtration resins for desalting and buffer exchange.  Agarose 4B, CL-4B, 6B and CL-6B are agarose and cross-linked agarose size exclusion chromatography base matrix that are frequently used for coupling affinity ligands to the matrix. Theses matrixes are not pre-activated and the user performs all steps in coupling. The columns available are our cross-linked dextran resin SpinOUT™ columns or the polyacrylamide SpinOUT™ G-Acryl columns for rapid desalting or buffer exchange applications and our prefilled size exclusion columns.

Hydrophobic Interaction Chromatography (HIC) resins, including butyl agarose, octyl agarose and phenyl agarose are offered as resin alone or in 1 and 5ml FPLC columns. A convenient HIC selection kit  featuring prepacked 1 ml columns of G-Trap™ Butyl Agarose 6 Fast Flow, G-Trap™ Octyl Agarose 6 Fast Flow, G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub) and G-Trap™ Phenyl Agarose 6 Fast Flow (Low Sub) is also available.

Ion Exchange Chromatography resins, including the cation exchanger resins CM agarose and SP agarose and the anionic exchanger resins DEAE agarose and Q agarose. These resins are offered as G-Trap™ FPLC columns, including a G-Trap™ Ion Exchange Selection kit, featuring a 1ml columns of G-Trap™ CM Agarose Fast FlowG-Trap™ SP Agarose Fast FlowG-Trap™ DEAE Agarose Fast Flow and G-Trap™ Q Agarose Fast Flow column.

Empty FPLC columns, empty low pressure glass columns, empty spin columns and empty gravity flow columns are available for researchers to pack their own columns or researchers can utilize G-Biosciences column packing service.

For small scale protein purification of specific proteins and protein complexes, four immunoprecipitation kits are offered. The Classic Immunoprecipitation, a Co-Immunoprecipitation, a Cross-link Immunoprecipitation and a Direct Immunoprecipitation.

 

Download the Protein Purification Handbook for a full list of related products

Download the Purification Resins Handbook for Complete Product List

G-Sep™ is a gel filtration resin comprised of ultrapure cross-linked dextran for desalting and buffer exchange in industrial applications. It exhibits high selectivity, superb resolution, low non-specific adsorption and robust chemical stability.   Quickly desalts, removes contaminants..
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The Carboxyl Coupling Resin consists of 6% cross-linked agarose with covalent linked diaminodipropylamine (DADPA) to generate a free primary amine at the end of a long spacer arm. This resin is also termed as EAH-Agarose.   Molecules, including proteins, peptides and nucleic acids, are c..
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Gene/Protein:
G-Biosciences’ Carboxyl Magnetic Beads are 1µm, uniform magnetic beads with surface functional group -COOH. The magnetic bead consists of a single-crystal Fe3O4 sphere core and dextran coating layer. Through chemical modification of dextran, the carboxyl groups (-COOH) are joined to..
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Immunoprecipitation (IP) is one of the most useful immunochemical techniques. IPs are routinely used to determine  the presence and quantity of an antigen, molecular weight of a polypeptide, rate of synthesis or degradation, identify certain post translational modifications and interaction..
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Immunoprecipitation (IP) is one of the most useful immunochemical techniques. IPs are routinely used to determine  the presence and quantity of an antigen, molecular weight of a polypeptide, rate of synthesis or degradation, identify certain post translational modifications and interaction..
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Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.     The Co-NTA Resin is specifically designed for the purif..
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Gene/Protein:
Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.    The Cobalt Chelating Resin is specifically designed for..
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Gene/Protein:
Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath et al (1), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.  The Copper Chelating Resin is specifically designed for the pu..
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Immunoprecipitation (IP) is one of the most useful immunochemical techniques. IPs are routinely used to determine  the presence and quantity of an antigen, molecular weight of a polypeptide, rate of synthesis or degradation, identify certain post translational modifications and interaction..
$0.00
Immunoprecipitation (IP) is one of the most useful immunochemical techniques. IPs are routinely used to determine  the presence and quantity of an antigen, molecular weight of a polypeptide, rate of synthesis or degradation, identify certain post translational modifications and interactions wit..
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Enterokinase (Recombinant) is highly purified recombinant bovine enterokinase obtained from E. Coli. Enterokinase is highly specific serine protease that hydrolysis peptide bond at the carboxyl side of lysine residue preceded by four aspartic acids (FLAG-tag). Enterokinase is used for removal ..
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Epoxy-Activated Agarose is preactivated resin used for affinity chromatography. Epoxy-Activated Agarose comprise of high density epoxy groups that form covalent bonds with ligands containing amine groups, thiol or hydroxyl groups.   Epoxide-Acivated Agarose is prepared by immobilization of ox..
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Gene/Protein:
Electroelution of nucleic acids and proteins has many advantages as it avoids centrifugation, vortexing, heating, precipitation and allows minimal manipulation of samples. Electroelution normally involves dialysis tubing, which results in extreme dilution of precious samples. G-Capsule™ is a s..
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G-Sep™ is a gel filtration resin comprised of ultrapure cross-linked dextran for desalting and buffer exchange in industrial applications. It exhibits high selectivity, superb resolution, low non-specific adsorption and robust chemical stability. Quickly desalts, removes contaminants and tr..
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G-Sep™ Agarose 4B is a gel filtration matrix formed from agarose beads that is available with 4% agarose content, designated G-Sep™ Agarose 4B.   The resin is a proven gel filtration base matrix and is routinely modified by researchers to couple affinity ligands.  The resin i..
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G-Sep™ Agarose 6B is a gel filtration matrix formed from agarose beads that is available with 6% agarose content, designated G-Sep™ Agarose 6B.   The resin is a proven gel filtration base matrix and is routinely modified by researchers to couple affinity ligands.  The resin i..
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G-Sep™ Agarose CL-4B resin is a cross-linked versions of our G-Sep™ Agarose. Cross-linking of the agarose results in chemically and physically more stable agarose beads that offer the same selectivity, but with better flow characteristics. Cross-linked agarose beads are resistant to..
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G-Sep™ Agarose CL-6B resin is a cross-linked versions of our G-Sep™ Agarose.  Cross-linking of the agarose results in chemically and physically more stable agarose beads that offer the same selectivity, but with better flow characteristics. Cross-linked agarose beads are r..
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G-Sep™ Agarose 6 Fast Flow (FF) is a gel filtration matrix formed from agarose beads that is based on our cross-linked 6% agarose.    The modification to the cross-linked 6% agarose to fast flow results in improved physical stability and chromatographic qualities.  The modific..
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G-Sep™ Butyl Agarose Fast Flow (FF) is a 6% cross-linked agarose with butyl groups for Hydrophobic Interaction Chromatography (HIC).  The resin is used to separate biomolecules on the basis of relative hydrophobicity.     G-Sep™ Agaroses for HIC are also available with o..
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G-Sep™ CM Agarose Fast Flow (FF) resin is a weak cation exchanger composed of highly cross-linked 6% agarose beads, with Carboxymethyl (CM) weak cation exchange groups.   The resin is extensively used for biomacromolecule separations in both research and industrial applications. The G-S..
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Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.  G-Sep™ Co IDA Agarose Fast Flow is specifically designed fo..
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G-Sep™ DEAE Agarose Fast Flow (FF) resin is a weak anion exchanger composed of highly cross-linked 6% agarose beads, with diethylaminoethyl (DEAE) weak anion exchange groups.   The resin is extensively used for biomacromolecule separations in both research and industrial applications. T..
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Gene/Protein:
G-Sep™ Hexyl Agarose is a 6% crosslinked agarose with propyl groups. It is suitable for use in Hydrophobic Interaction Chromatography (HIC) to separate or purify biomolecules based on their relative hydrophobicity.   G-Sep™ Agaroses for HIC are also available with Propyl, Buty..
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Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.  G-Sep™ Ni IDA Agarose Fast Flow is specifically designed fo..
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G-Sep™ Octyl Agarose Fast Flow (FF) is a 6% cross-linked agarose with octyl groups for Hydrophobic Interaction Chromatography (HIC).   The resin is used to separate biomolecules on the basis of relative hydrophobicity.     G-Sep™ Agaroses for HIC are also available with&..
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G-Sep™ Phenyl Agarose Fast Flow (FF) is a 6% cross-linked agarose with phenyl groups for Hydrophobic Interaction Chromatography (HIC).   The resin is used to separate biomolecules on the basis of relative hydrophobicity.     G-Sep™ Phenyl Agarose Fast Flow is available i..
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Gene/Protein:
G-Sep™Propyl Agarose is a 6% crosslinked agarose with propyl groups. It is suitable for use in Hydrophobic Interaction Chromatography (HIC) to separate or purify biomolecules based on their relative hydrophobicity.   G-Sep™ Agaroses for HIC are also available with Hexyl, Butyl..
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G-Sep™ Q Agarose Fast Flow (FF) resin is a strong anion exchanger composed of highly cross-linked 6% agarose beads, with quaternary ammonium (Q) strong anion exchange groups.   The resin is extensively used for biomacromolecule separations in both research and industrial applications. T..
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Size Exclusion Chromatography or gel filtration is a widely used technique for separation of proteins and other molecules based on their sizes.  G-Sep™ Size Exclusion Columns are composed of high performance rigid resin made up of co-polymer of agarose and dextran. The Columns are availa..
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G-Sep™ SP Agarose Fast Flow (FF) resin is a strong cation exchanger composed of highly cross-linked 6% agarose beads, with Sulphopropyl (SP) strong cation exchange groups.   The resin is extensively used for biomacromolecule separations in both research and industrial applications. The ..
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G-Trap™ Butyl Agarose 6 Fast Flow are prepacked ready to use columns for seperation of biomolecules via Hydrophobic Interaction chromatography (HIC).    Biomolecules are separated on the HIC column based upon the hydrophobicity of the ligand attached to the resin, the distribution ..
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G-Trap™ CM Agarose Fast Flow is ready to use prepacked column used for separation of proteins and other biomolecules based on charge. The resin in G-Trap™ CM Agarose Fast Flow is a weak cation exchanger composed of highly crosslinked 6% agarose beads coupled with carboxymethyl (CM) weak ..
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G-Trap™ Co IDA Agarose Fast Flow columns are ready to use prepacked affinity columns used for purification of proteins based on immobilized metal ion affinity chromatography (IMAC).   IMAC is based on interaction of cations of transition metals such as Ni2+, Co2+ with protein residues h..
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G-Trap™ FPLC Columns are empty columns designed for fast protein liquid chromatography. G-Trap™ FPLC Columns are made of polypropylene and come in 1 ml and 5 ml sizes.   Features: Column material: polypropylene (PP) Frit material: Ultra high molecular weigh..
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G-Trap™ GT-100 Desalting Column is prepacked ready to use column for separation of low molecular weight substances from high molecular weight substances based on size exclusion chromatography.    G-Trap™ GT-100 Desalting Columns are mostly used for removal of salt or buffer e..
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G-Trap™ GT-600 Desalting Column is prepacked ready to use column for separation of low molecular weight substances from high molecular weight substances based on size exclusion chromatography.    G-Trap™ GT-600 Desalting Columns are mostly used for removal of salt or buffer e..
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G-Trap™ HIC Selection Kit consists of 4 prepacked 1 ml columns (G-Trap™ HIC Columns) with matrix or resin for hydrophobic interaction chromatography. The kit consists of 4 pepacked columns namely G-Trap™ Butyl Agarose 6 Fast Flow, G-Trap™ Octyl Agarose 6 Fast Flow, G-Trap&tra..
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G-Trap™ Ion Exchange Selection Kit contains 4 different types of 1 ml G-Trap™ Ion Exchange Agarose Fast Flow Columns which are used for initial screening of the suitable ion exchange resin and/or pH or ionic strength optimization of start and elution buffer to separate the target protein..
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G-Trap™ Ni IDA Agarose Fast Flow columns are ready to use prepacked affinity columns used for purification of proteins based on immobilized metal ion affinity chromatography (IMAC).   IMAC is based on interaction of cations of transition metals such as Ni2+, Co2+ with protein resid..
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G-Trap™ Octyl Agarose 6 Fast Flow are prepacked ready to use columns for seperation of biomolecules via Hydrophobic Interaction chromatography (HIC).    Biomolecules are separated on the HIC column based upon the hydrophobicity of the ligand attached to the resin, the distribution ..
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G-Trap™ Q Agarose Fast Flow is ready to use prepacked column used for seperation of proteins and other biomolecules based on charge. The resin in G-Trap™ Q Agarose Fast Flow is a strong anion exchanger composed of highly crosslinked 6% agarose beads coupled with quaternary ammonium ..
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G-Trap™ SP Agarose Fast Flow is ready to use prepacked column used for seperation of proteins and other biomolecules based on charge. The resin in G-Trap™ SP Agarose Fast Flow is a strong cation exchanger composed of highly crosslinked 6% agarose beads coupled with sulphopropyl (SP)..
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HOOK™ 6X His Protein Purification kit allows for the purification of soluble, 6X His tagged protein from bacterial cultures. The bacteria are first lysed with Bacterial PE LB™ and PE LB™-Lysozyme to release total soluble protein, whilst maintaining the structure and activity o..
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Gene/Protein:
HOOK™ 6X His Protein Purification kit allows for the purification of soluble, 6X His tagged protein from yeast cultures. The yeast are first lysed with Yeast PE LB™ and LongLife™ Zymolyase® to release total soluble protein, whilst maintaining the structure and activity of ..
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HOOK™ 6X His Protein Spin Purification kit allows for the rapid purification of soluble, 6X His tagged protein from bacterial cultures.    The bacteria are first lysed with Bacterial PE LB™ and PE LB™-Lysozyme to release total soluble protein, whilst maintaining the str..
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Gene/Protein:
HOOK™ 6X His Protein Spin Purification kit allows for the rapid purification of soluble, 6X His tagged protein from yeast cultures. The yeast are first lysed with Yeast PE LB™ and LongLife™ Zymolyase® to release total soluble protein, whilst maintaining the structure and a..
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An important tool in research is the generation of peptide and protein affinity columns for the purification of antibodies and for the discovery of important interacting proteins and cofactors. G-Biosciences offers a complete kit for the coupling of peptides and proteins to agarose through thei..
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Gene/Protein:
HOOK™ Cell Surface Protein Isolation kit uses G-Biosciences HOOK™ biotin labeling and purification technology in conjunction with our Mammalian Cell PE LB™ lysis buffer to conveniently label cell surface proteins and isolate them for further analysis, including Western blotting. ..
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HOOK™ GST Protein Purification kit allows for the purification of soluble, GST tagged protein from bacterial cultures.  The bacteria are first lysed with Bacterial PE LB™ and PE LB™-Lysozyme to release total soluble protein, whilst maintaining the structure and activity of the..
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Gene/Protein:
HOOK™ GST Protein Purification kit allows for the purification of soluble, GST tagged protein from yeast cultures. The yeast are first lysed with Yeast PE LB™ and LongLife™ Zymolyase® to release total soluble protein, whilst maintaining the structure and activity of the pr..
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HOOK™ GST Protein Spin Purification kit allows for the rapid purification of soluble, GST tagged protein from bacterial cultures. The bacteria are first lysed with Bacterial PE LB™ and PE LB™-Lysozyme to release total soluble protein, whilst maintaining the structure and activ..
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Gene/Protein:
HOOK™ GST Protein Spin Purification kit allows for the rapid purification of soluble, GST tagged protein from yeast cultures. The yeast are first lysed with Yeast PE LB™ and LongLife™ Zymolyase® to release total soluble protein, whilst maintaining the structure and activit..
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The expression of recombinant proteins is a popular and routinely used technique in protein studies.  The expression of recombinant proteins often has one drawback and that is the recombinant proteins aggregate and form inclusion bodies, especially when expressed at high levels.  The aggre..
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Gene/Protein:
The expression of recombinant proteins is a popular and routinely used technique in protein studies.  The expression of recombinant proteins often has one drawback and that is the recombinant proteins aggregate and form inclusion bodies, especially when expressed at high levels.  The aggre..
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G-Biosciences Immobilized Amino Acids are prepared by coupling amino acid to preactivated 6% cross-linked agarose. The covalent coupling to agarose (resin) is achieved by cross-linking amino group of the amino acid to the preactivated resin   Click here to request bulk or custom sizes. Feat..
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​Amoxicillin is the 4-hydroxy analog of ampicillin and has similar actions and uses as of ampicillin. It shows specific antibacterial activity against Enterococcus faecalis, Heliobacter pylori, and Salmonella species.  Immobilized Amoxicillin  is made by covalently coupling amoxicill..
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Gene/Protein:
Ampicillin is an inhibitor of bacterial cell wall synthesis which acts by inhibiting the cross-linking of peptidoglycan. It is used in the selection of antibiotic cell hybrids, and has a high rate of action agaisnt many gram negative bacterial strains.   Immobilized Ampicillin is made by co..
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Gene/Protein:
G-Biosciences immobilized antibiotics are prepared by coupling antibiotic to preactivated 6% cross-linked agarose. The covalent coupling to agarose (resin) is achieved by cross-linking amino or carboxyl group depending upon the antibiotic that is coupled to the resin.   Click here to reques..
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Apramycin, an aminoglycoside antibiotic, is produced by a strain of Streptomyces tenebrarius. Apramycin is a potent inhibitor of protein synthesis in bacteria in vivo and in vitro.    Immobilized Apramycin  is made by covalently coupling apramycin to 6% cross linked agarose and ..
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Gene/Protein:
Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=1015 M-1). Biotin and avidin interaction is rapid and once the bond is established it can survive up to 3M guanidine-hydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by d..
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Bacitracin is a peptide antibiotic and shows antibacterial activity against gram-positive bacteria. It inhibits bacterial cells wasll synthesis by inhibiting dephosphorylation of lipid pyrophosphate.   Immobilized Bacitracin is made by covalently coupling bacitracin to 6% cross linked aga..
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Cephalexin is a cephalosporin antibiotic which disrupts the synthesis of the peptidoglycan layer of bacterial cell walls. Peptidoglycan synthesis is aided by transpeptidase known as pencillin-binding proteins (PBP). Cephalexin inhibits PBP3 and other penicillin-binding proteins (PBPs).  Immobi..
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​Ceftazidime inhibits enzymes which are responsible for bacterial cell-wall synthesis, mainly penicillin binding protein 3 (PBP3). A broad range of gram-negative bacteria are susptible to ceftazidime including strains resistant to gentamicin and other aminoglycosides. Immobilized ​Ceftazidime ..
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​Cloxacillin antibiotic binds to specific penicillin-binding proteins (PBPs) located inside the bacterial cells wall and inhibits the third amd last stage of bacterial cell wall synthesis. It is used against stphyloccoci that produce beta-lactamase. Immobilized Cloxacillin is made by cov..
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D-Alanine is the non-proteinogenic form of alanine which is used in bacterial cell wall biosynthesis. Immobilized D-Alanine is prepared by covalently coupling amiono group of D-alanine to the preactivated 6% cross-linked agarose. Click here to request bulk or custom sizes.   Features: ..
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Gene/Protein:
G-Biosciences Immobilized D-Galactose is for the purification of lectins, galactosidases and other galactose-, N-acetylgalactosamine- or carbohydrate binding molecules.  Specific applications include the purification of galactosidases, C-type lectins, enterotoxins and cholera toxin.   ..
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Gentamicin inhibits growth of many Gram-positive and Gram-negative microorganisms. It does so by inhibiting protein synthesis by binding to the L6 protein of the 30S ribosomal subunit. It is also effective against mycoplasma.  Immobilized Gentamicin is prepared by covalently coupling gent..
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Hygromycin B is an aminocyclitol with a wide spectrum of activity that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. It is produced by the bacterium Streptomyces hygroscopicus. Immobilized Hygromycin B is made by covalently coupling hygromycin B to 6% cro..
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Jacalin, or Artocarpus integrifolia lectin, is a tetrameric two-chain lectin with a molecular weight of 66kDa. Jacalin is a α-D-galactose binding lectin purified from jack-fruit (Artocarpus integrifolia) seeds.    Click here to request bulk or custom sizes.   Features ..
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L-Alanine  is an α-amino acid which is used in the biosynthesis of proteins. Immobilized L-Alanine is prepared by covalently coupling amiono group of L-Alanine to the preactivated 6% cross-linked agarose. Click here to request bulk or custom sizes.   Features: L-Alanine c..
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Gene/Protein:
L-Arginine  is an α-amino acid  which is used in the biosynthesis of proteins. Immobilized L-Arginine is prepared by covalently coupling amiono group of L-Arginine to the preactivated 6% cross-linked agarose. Click here to request bulk or custom sizes.   Features: ..
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L-Asparagine is an α-amino acid which is used in the biosynthesis of proteins. Immobilized L-Asparagine is prepared by covalently coupling amiono group of L-Asparagine to the preactivated 6% cross-linked agarose. Click here to request bulk or custom sizes. Features: L-Asparagi..
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L-Aspartic acid is an α-amino acid which is used in the biosynthesis of proteins. Immobilized L-Aspartic acid is prepared by covalently coupling amiono group of L-Aspartic acid to the preactivated 6% cross-linked agarose. Click here to request bulk or custom sizes. ​ Features: ..
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L-Cysteine is semi-essential thiol containing amino acid which is used in the biosynthesis of proteins. It is oxidized to form cystine. Immobilized L-cysteine is prepared by covalently coupling amiono group of L-cysteine to the preactivated 6% cross-linked agarose. Click here to request bulk ..
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