Purification & Chromatography
G-Biosciences offers protein purification and protein chromatography reagents, including agarose resins for coupling researchers ligands, via versus coupling chemistries, or pre-coupled ligands for purification of specific protein tags. We offer activated agarose resins for conjugation of proteins and biomolecules through amines, carbohydrates, carboxyls, sulfhydryls, hydroxyls and activated hydrogens for coupling your ligand to agarose or magnetic beads. We offer premade protein purification resins for the isolation of specific protein groups or protein tags, including streptavidin and avidin purification, biotinylated protein purification, calmodulin binding protein (CBP) purification, C-Reactive protein purification, GST tagged protein purification, 6X His tagged protein purification, Heparin binding protein purificaiton and glycoprotein purification with immobilized lectins. For antibody purification resins, Protein A resin, Protein G resin and Protein A/G resin are offered in multiple formats, including resin, spin columns and FPLC columns. Several antibody purification products based on our proprietary Pearl™ IgG Purification resin are offered, including an antibody clean-up kit and a monoclonal antibody purification kit.
Gel filtration resins or size exclusion resins are also offered as resin alone or as spin format columns. The resins offered are G-Sep™-25 and G-Sep™-50 that are equivalents to the well established gel filtration resins for desalting and buffer exchange. Agarose 4B, CL-4B, 6B and CL-6B are agarose and cross-linked agarose size exclusion chromatography base matrix that are frequently used for coupling affinity ligands to the matrix. Theses matrixes are not pre-activated and the user performs all steps in coupling. The columns available are our cross-linked dextran resin SpinOUT™ columns or the polyacrylamide SpinOUT™ G-Acryl columns for rapid desalting or buffer exchange applications and our prefilled size exclusion columns.
Hydrophobic Interaction Chromatography (HIC) resins, including butyl agarose, octyl agarose and phenyl agarose are offered as resin alone or in 1 and 5ml FPLC columns. A convenient HIC selection kit featuring prepacked 1 ml columns of G-Trap™ Butyl Agarose 6 Fast Flow, G-Trap™ Octyl Agarose 6 Fast Flow, G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub) and G-Trap™ Phenyl Agarose 6 Fast Flow (Low Sub) is also available.
Ion Exchange Chromatography resins, including the cation exchanger resins CM agarose and SP agarose and the anionic exchanger resins DEAE agarose and Q agarose. These resins are offered as G-Trap™ FPLC columns, including a G-Trap™ Ion Exchange Selection kit, featuring a 1ml columns of G-Trap™ CM Agarose Fast Flow, G-Trap™ SP Agarose Fast Flow, G-Trap™ DEAE Agarose Fast Flow and G-Trap™ Q Agarose Fast Flow column.
Empty FPLC columns, empty low pressure glass columns, empty spin columns and empty gravity flow columns are available for researchers to pack their own columns or researchers can utilize G-Biosciences column packing service.
For small scale protein purification of specific proteins and protein complexes, four immunoprecipitation kits are offered. The Classic Immunoprecipitation, a Co-Immunoprecipitation, a Cross-link Immunoprecipitation and a Direct Immunoprecipitation.
L-Glutamic acid is an α-amino acid that is used in the biosynthesis of proteins.
Immobilized L-Glutamic acid is prepared by covalently coupling amiono group of L-glutamic acid to the preactivated 6% cross-linked agarose.
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Features:
L-Glutam..
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L-Glutamine is an α-amino acid that is used in the biosynthesis of proteins.
Immobilized L-Glutamine is prepared by covalently coupling amiono group of L-glutamine to the preactivated 6% cross-linked agarose.
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Features:
L-Glutamine coupled to..
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L-Glycine is an α-amino acid that is used in the biosynthesis of proteins.
Immobilized L-Glycine is prepared by covalently coupling amiono group of L-glycine to the preactivated 6% cross-linked agarose.
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Features:
L-Glycine co..
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L-Histidine is an α-amino acid which is used in the biosynthesis of proteins.
Immobilized L-Histidine is prepared by covalently coupling amiono group of L-histidine to the preactivated 6% cross-linked agarose.
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Features:
L-Histidine co..
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L-Isoleucine is an α-amino acid which is used in the biosynthesis of proteins.
Immobilized L-Isoleucine is prepared by covalently coupling amiono group of L-isoleucine to the preactivated 6% cross-linked agarose.
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Features:
L-Iso..
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L-Leucine is an α-amino acid which is used in the biosynthesis of proteins.
Immobilized L-Leucine is prepared by covalently coupling amiono group of L-leucine to the preactivated 6% cross-linked agarose.
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Features:
L-Leucine coupled to 6% cr..
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L-Lysine is an α-amino acid which is used in the biosynthesis of proteins.
Immobilized L-Lysine is prepared by covalently coupling amiono group of L-lysine to the preactivated 6% cross-linked agarose.
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Features:
L-Lysine coupled to 6% c..
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L-Methionine is an α-amino acid which is used in the biosynthesis of proteins.
Immobilized L-Methionine is prepared by covalently coupling amiono group of L-methionine to the preactivated 6% cross-linked agarose.
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Features:
L-Methionine c..
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L-Phenylalanine is an essential α-amino acid which is used in the biosynthesis of proteins.
Immobilized L-Phenylalanine is prepared by covalently coupling amiono group of L-phenylalanine to the preactivated 6% cross-linked agarose.
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L-Proline is an α-amino acid which is used in the biosynthesis of proteins.
Immobilized L-Proline is prepared by covalently coupling amiono group of L-Proline to the preactivated 6% cross-linked agarose.
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Features:
L-P..
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L-Serine is an α-amino acid which is used in the biosynthesis of proteins.
Immobilized L-Serine is prepared by covalently coupling amiono group of L-serine to the preactivated 6% cross-linked agarose.
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Features:
L-Serine c..
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L-Threonine is an essential α-amino acid which is used in the biosynthesis of proteins.
Immobilized L-Threonine is prepared by covalently coupling amiono group of L-threonineto the preactivated 6% cross-linked agarose.
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Features:..
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L-Tryptophan is an essential α-amino acid which is used in the biosynthesis of proteins.
Immobilized L-Tryptophan is prepared by covalently coupling amiono group of L-tryptophan to the preactivated 6% cross-linked agarose.
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L-Tyrosine is an α-amino acid which is used in the biosynthesis of proteins.
Immobilized L-Tyrosine is prepared by covalently coupling amiono group of L-tyrosine to the preactivated 6% cross-linked agarose.
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Features:
L-Tyrosine ..
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L-Valine is an essential α-amino acid which is used in the biosynthesis of proteins.
Immobilized L-Valine is prepared by covalently coupling amiono group of L-valine to the preactivated 6% cross-linked agarose.
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Features:
L..
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Neomycin is a bactericidal aminoglycoside antibiotic. It binds to the 30S ribosome of susceptible organisms and interferes with mRNA binding and acceptor tRNA sites and results in the production of non-functional or toxic peptides.
Immobilized Neomycin is prepared by covalently coupling neomycin to..
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Penicillin G is a naturally occurring beta lactam antibiotic produced by the Penicillium fungus. Penicillin G works by inhibiting cell wall production in non-resistant cells by breaking the links between peptidogylocans and causing the production of cell wall damaging autolysins.
Immobilized ..
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Immobilized Procainamide is designed for affinity purification of butyrylcholinesterase (BuChE), which is also known as non-specific cholinesterase.
Butyrylcholinesterase can be purified by batch adsorption on immobilized Procainamide followed by ion-exchange chromatography on DEAE-Sepharose column..
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-500x500h.jpg "Immobilized Protein G")
Gene/Protein:
Immobilized protein G is for binding the constant domains of immunoglobulin (Ig) molecules (see Figure 1). It is ideal for the easy one-step purification of classes, subclasses and fragments of immunoglobulins for biological fluids and cell culture media. Protein G is a modified form of Streptococca..
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-500x500h.jpg "Immobilized Streptavidin Resin")
Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10¹âµM-¹) and streptavidin (Ka=10¹âµM-¹). Biotin and (strept)avidin interaction is rapid. Once the bond is established, it can survive up to 3M guanid..
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Streptomycin Sulfate is an aminoglycoside antibiotic and has a bactericidal action against many gram-negative bacteria. It binds to the S12 protein on the ribosomal 30S subunit and inhibits protein synthesis
Immobilized Streptomycin is made by covalentlly coupling streptomycin to..
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Sulfadimidine is a bacteriostatic antimicrobial sulfur drug. It blocks the synthesis of dihydrofolic acid by inhibiting the enzyme dihydropteroate synthase. Sulfadimidine is a competitive inhibitor of bacterial para-aminobenzoic acid (PABA), which is required for bacterial synthesis of folic acid.
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Tetracycline is a bacteriostatic antibiotic with activity against both gram-positive and gram-negative bacteria. Within the cell tetracycline binds reversibly to the 30S subunit of the ribosome, preventing the binding of aminoacyl tRNA and inhibiting protein synthesis and hence cell growth.
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MES SDS Buffer Kit is a complete kit to resolve small- to medium-sized proteins under denaturing conditions on Bis-Tris gels.
Features
MES SDS Buffer Kit includes MES SDS Running Buffer [20X], DTT [0.5 M,10X], Protein Antioxidant [200X] and LDS Sample Loading Buffer [4X]
The kit is comp..
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Lectins are proteins or glycoproteins that have at least one noncatalytic domain that binds specifically and reversibly to monosaccharides or oligosaccharides. Lectins are important as they are found in applications in science, medicine, agriculture, pharmaceutical companies, biotechnology and other..
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Lectins are proteins or glycoproteins that have at least one noncatalytic domain that binds specifically and reversibly to monosaccharides or oligosaccharides. Lectins are important as they are found in applications in science, medicine, agriculture, pharmaceutical companies, biotechnology and other..
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Gene/Protein:
NHS-Activated Agarose consists of 6% cross-linked agarose that has been activated by the addition of a reactive NHS (N-hydroxysuccinimide) group. The NHS group forms covalent, chemically stable amide bonds with ligands that contain primary amines. The NHS-Activated Agarose also contains a spacer arm..
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-500x500h.jpg "Nickel Chelating Resin")
Gene/Protein:
Immobilized metal affinity chromatography (IMAC) resin utilizing nickel (Ni2+) for the purification of 6x histidine tagged proteins.
This resin binds to six histidine residues (6x His), a common tag used in protein purification. The resin consists of iminodiacetate coupled to 6% cross-l..
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ρ-Aminobenzamidine Agarose primary application is for the removal and/or purification of trypsin-like proteases. ρ-aminobenzamidine (PAB) is a synthetic inhibitor of trypsin-like proteases and has been covalently coupled to 6% crosslinked agarose.
ρ-aminobenzamidine agaros..
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The Pearl™ IgG Purification kits are designed for the one step purification of IgG (Immunoglobulin G) antibodies from serum. The supplied Pearl™ IgG Purification Resin binds the high abundant, non-IgG proteins (i.e albumin) and allows the IgG molecules to pass through in a physiolog..
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Gene/Protein:
Protein A is a cell-wall receptor which is present on cell wall of various starins of Staphyloccocus aureus. Protein A contains four high-affinity binding sites for interacting with the Fc region of immunoglobulin Gs of various species including mouse, rabbit and human. Protein A is used for making ..
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Gene/Protein:
G-Biosciences' Protein G Magnetic Beads are Fe3O4 beads coated with dextran to produce highly uniform, 1µm beads. Recombinant Protein G is covalently coupled to the dextran coat to produce an enhanced alternative to agarose slurries for immunoprecipitation experiments. G-Biosciences m..
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The SDC™ (Steroid/ Drug/ Compound) Immobilization kit is designed for the immobilization of steroids, drugs and chemical compounds that lack primary amines, sulfhydryls, carbonyls and other common coupling groups to a solid-phase agarose support for the use in affinity purification. The ..
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Protein Purification STRATEGY™ consists of numerous fractionation and chromatography techniques to aid researchers to develop a suitable purification system for specific and novel proteins. A major issue in purifying a novel protein is that the characteristics of the protein to be pur..
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Gene/Protein:
The Sulfhydryl Immobilization Kit for Peptides is designed for the simple and efficient coupling of sulfhydryl-containing peptides to a solid agarose support. The Sulfhydryl Coupling Resin Columns utilizes iodoacetyl groups that specifically react with free sulfhydryls to form covalent, permanent th..
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The Sulfhydryl Immobilization Kit for Proteins is a complete kit designed for the simple and efficient coupling of proteins to a solid agarose support. The Sulfhydryl Coupling Resin Columns utilizes iodoacetyl groups that specifically react with free sulfhydryls to form covalent, permanent thioether..
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Thiophilic adsorption or thiophilic chromatography is a routinely used technique for the low cost, simple purification of immunoglobulins. Thiophilic adsorption was first developed by Porath et al1 in 1984 and is a group specific, salt-dependent purification technique that has distinct affinit..
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Thiophilic adsorption or thiophilic chromatography is a routinely used technique for the low cost, simple purification of immunoglobulins. Thiophilic adsorption was first developed by Porath et al1 in 1984 and is a group specific, salt-dependent purification technique that has distinct affinit..
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G-Biosciences’ Amine Magnetic Beads are 1µm, uniform magnetic beads with an amine (-NH2) surface functional group. The magnetic beads consist of a single-crystal Fe3O4 sphere core and dextran coating layer. Through chemical modification of dextran, the primary amino group (-NH2) are..
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Gene/Protein:
Capillary electrophoresis buffer [10X] is composed of 1M TAPS and 14mM EDTA with pH 7.6±0.4. Used for DNA sequencing.
TAPS is a zwitterionic buffering agent, effective in making buffer solutions in pH 8.4±0.7. TAPS is used in capillary electrophoresis to separate and analyze DNA and..
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Gene/Protein:
G-Biosciences offers G-Trap™ Immobilized Amino Acids resin in a pre-packed column format. Immobilized Amino Acids are prepared by coupling amino acid to preactivated 6% cross-linked agarose. The covalent coupling to agarose (resin) is achieved by cross-linking the amino or..
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G-Trap™ Protein G are prepacked ready to use columns for affinity purification of Immunoglobulins G. The column is packed with resin, which is covalently coupled to recombinant Protein G.
Recombinant Protein G is coupled covalently by proprietary coupling method to highly crossli..
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G-Trap™ rProtein A FF are prepacked ready to use columns for purification of IgG antibodies.
The column is packed with fast flow resin, which is covalently coupled to recombinant Protein A. Recombinant Protein A is coupled covalently by proprietary coupling method to highly crosslinke..
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Gene/Protein:
Glutathione Resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST) tag. The resin consists of reduced glutathione (GSH) covalently coupled to 6% cross-linked agarose, via a 10-carbon spacer arm. The resin has a binding capacity of >40mg GST/ml resin. Su..
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The amine reactive HOOK™ Activated Agarose is 6% agarose that has been activated to generate reactive aldehyde groups. The aldehyde groups of the agarose react spontaneously with primary amines, located at the N-terminus of proteins or in lysine residues, to form intermediate Schiff Base compl..
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-500x500h.jpg "Immobilized Biotin Resin")
Gene/Protein:
Immobilized Biotin Resin is designed for the high affinity chromatography purifications of avidin, streptavidin and Neutravidin protein. The resin consists of biotin coupled to 6% cross-linked agarose.
Biotin, a 244 Dalton vitamin (Vitamin H) molecule, exhibits an extraordin..
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Gene/Protein:
Immobilized L-Tartrate is designed for affinity purification of L-Tartrate binding proteins such as human prostatic acid phosphatase.
Features:
Matrix: 6% cross-linked Agarose
Coupling is covalent through linkage of hydroxyl group of tartrate to the pre-activated agarose.
Extent ..
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Gene/Protein:
G-Biosciences' Immobilized p-Aminophenyl Phosphoryl Choline consists of a phosphoryl choline covalently linked to beaded agarose and is designed for the purification of C-reactive protein from plasma, ascites and other biological fluids.
CRP, C-reactive protein, is a pentameric prot..
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-500x500h.jpg "Immobilized Protein A")
Gene/Protein:
Immobilized Protein A is for binding the constant domains of immunoglobulin (Ig) molecules (see Figure 1). Protein A is coupled to agarose beads by reductive amination method that provides high coupling efficiency for immunoglobulins and minimal protein A leaching (<5ng protein A/ml). Our im..
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Gene/Protein:
The Ni-NTA Resin in a high binding, high capacity nickel charged IMAC resin that purifies recombinant proteins with the polyhistidine (6XHis) sequence. The resin is 6% cross-linked agarose for excellent structural integrity with a high capacity of 20-40μmoles Ni2+/ml resin that results..
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Gene/Protein:
Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.
The SpinOUT™ GT-100, GT-600 and GT-1200 columns are versatile, spin-format columns for the desalting and buffer exchange of peptide, prot..
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The SpinOUT™ G-Acryl columns are versatile, spin-format columns for the desalting and buffer exchange of peptide and protein and other macromolecule solutions ranging from 5µl through to 3ml sample volumes. The SpinOUT™ columns are available in three MWCO sizes for >1,800,..
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The Sulfhydryl Coupling Resin, or Iodoacetyl resin, is designed for the simple and efficient coupling of peptides and proteins to a solid 6% agarose support through free sulfhydryl groups (-SH). The iodoacetyl groups of the Sulfhydryl Coupling Resin specifically react with free sulfhydryl..
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Gene/Protein:
TAPS is a zwitterionic buffering agent, effective in making buffer solutions in pH 8.4±0.7. TAPS is used in capillary electrophoresis to separate and analyze DNA and for separating dyes using planar chromatography. The buffer is sterile, economical and ready to use. TAPS is an abbreviati..
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-500x500h.jpg "Zinc Chelating Resin")
Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. The Zinc Chelating Resin is specifically designed for the purificati..
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A broad range, bacterial, 100X concentrated, ready-to-use protease inhibitor cocktail of AEBSF, 1,10-Phenathroline, Benzamidine, Iodoacetamide, Pepstain A and PMSF that offers greater protection for recombinant proteins expressed and purified from bacteria. The protease inhibitor cocktail inhibits b..
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Aprotinin is a monomeric globular protein which inhibits several serine proteases including trypsin, chymotrypsin, plasmin and kallikrein. Furthermore, its action on kallikrein leads to inhibition of formation of factorXIIa.The binding of aprotinin to proteases is reversible with dissociation of apr..
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Gene/Protein:
G-Biosciences Immobilized Pepstatin is for the purification of cathepsins, pepsin, bacterial aspartic proteases, HIV proteases and other molecules that bind pepstatin.
Pepstatin is isovaleryl-Val-Val-AHMHA-Ala-AHMHA where AHMHA= (3S, 4S)-4-amino-3-hydroxy-6-methyl-heptanoic acid a..
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