menu
Your Cart

Purification & Chromatography

G-Biosciences offers protein purification and protein chromatography reagents, including agarose resins for coupling researchers ligands, via versus coupling chemistries, or pre-coupled ligands for purification of specific protein tags. We offer activated agarose resins for conjugation of proteins and biomolecules through amines, carbohydrates, carboxyls, sulfhydryls, hydroxyls and activated hydrogens for coupling your ligand to agarose or magnetic beads. We offer premade protein purification resins for the isolation of specific protein groups or protein tags, including streptavidin and avidin purification, biotinylated protein purification, calmodulin binding protein (CBP) purification, C-Reactive protein purification, GST tagged protein purification, 6X His tagged protein purification, Heparin binding protein purificaiton and glycoprotein purification with immobilized lectins. For antibody purification resins, Protein A resin, Protein G resin and Protein A/G resin are offered in multiple formats, including resin, spin columns and FPLC columns.  Several antibody purification products based on our proprietary Pearl™ IgG Purification resin are offered, including an antibody clean-up kit and a monoclonal antibody purification kit.

Gel filtration resins or size exclusion resins are also offered as resin alone or as spin format columns. The resins offered are G-Sep™-25 and G-Sep™-50 that are equivalents to the well established gel filtration resins for desalting and buffer exchange.  Agarose 4B, CL-4B, 6B and CL-6B are agarose and cross-linked agarose size exclusion chromatography base matrix that are frequently used for coupling affinity ligands to the matrix. Theses matrixes are not pre-activated and the user performs all steps in coupling. The columns available are our cross-linked dextran resin SpinOUT™ columns or the polyacrylamide SpinOUT™ G-Acryl columns for rapid desalting or buffer exchange applications and our prefilled size exclusion columns.

Hydrophobic Interaction Chromatography (HIC) resins, including butyl agarose, octyl agarose and phenyl agarose are offered as resin alone or in 1 and 5ml FPLC columns. A convenient HIC selection kit  featuring prepacked 1 ml columns of G-Trap™ Butyl Agarose 6 Fast Flow, G-Trap™ Octyl Agarose 6 Fast Flow, G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub) and G-Trap™ Phenyl Agarose 6 Fast Flow (Low Sub) is also available.

Ion Exchange Chromatography resins, including the cation exchanger resins CM agarose and SP agarose and the anionic exchanger resins DEAE agarose and Q agarose. These resins are offered as G-Trap™ FPLC columns, including a G-Trap™ Ion Exchange Selection kit, featuring a 1ml columns of G-Trap™ CM Agarose Fast FlowG-Trap™ SP Agarose Fast FlowG-Trap™ DEAE Agarose Fast Flow and G-Trap™ Q Agarose Fast Flow column.

Empty FPLC columns, empty low pressure glass columns, empty spin columns and empty gravity flow columns are available for researchers to pack their own columns or researchers can utilize G-Biosciences column packing service.

For small scale protein purification of specific proteins and protein complexes, four immunoprecipitation kits are offered. The Classic Immunoprecipitation, a Co-Immunoprecipitation, a Cross-link Immunoprecipitation and a Direct Immunoprecipitation.

 

Download the Protein Purification Handbook for a full list of related products

Download the Purification Resins Handbook for Complete Product List

Cephalexin is a cephalosporin antibiotic which disrupts the synthesis of the peptidoglycan layer of bacterial cell walls. Peptidoglycan synthesis is aided by transpeptidase known as pencillin-binding proteins (PBP). Cephalexin inhibits PBP3 and other penicillin-binding proteins (PBPs).  Immobi..
$0.00
​Ceftazidime inhibits enzymes which are responsible for bacterial cell-wall synthesis, mainly penicillin binding protein 3 (PBP3). A broad range of gram-negative bacteria are susptible to ceftazidime including strains resistant to gentamicin and other aminoglycosides. Immobilized ​Ceftazidime ..
$0.00
​Cloxacillin antibiotic binds to specific penicillin-binding proteins (PBPs) located inside the bacterial cells wall and inhibits the third amd last stage of bacterial cell wall synthesis. It is used against stphyloccoci that produce beta-lactamase. Immobilized Cloxacillin is made by cov..
$0.00
D-Alanine is the non-proteinogenic form of alanine which is used in bacterial cell wall biosynthesis. Immobilized D-Alanine is prepared by covalently coupling amiono group of D-alanine to the preactivated 6% cross-linked agarose. Click here to request bulk or custom sizes.   Features: ..
$0.00
G-Biosciences Immobilized D-Galactose is for the purification of lectins, galactosidases and other galactose-, N-acetylgalactosamine- or carbohydrate binding molecules.  Specific applications include the purification of galactosidases, C-type lectins, enterotoxins and cholera toxin.   Th..
$0.00
Gentamicin inhibits growth of many Gram-positive and Gram-negative microorganisms. It does so by inhibiting protein synthesis by binding to the L6 protein of the 30S ribosomal subunit. It is also effective against mycoplasma.  Immobilized Gentamicin is prepared by covalently coupling gent..
$0.00
Hygromycin B is an aminocyclitol with a wide spectrum of activity that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. It is produced by the bacterium Streptomyces hygroscopicus. Immobilized Hygromycin B is made by covalently coupling hygromycin B to 6% cro..
$0.00
Jacalin, or Artocarpus integrifolia lectin, is a tetrameric two-chain lectin with a molecular weight of 66kDa. Jacalin is a α-D-galactose binding lectin purified from jack-fruit (Artocarpus integrifolia) seeds.    Click here to request bulk or custom sizes.   Features ..
$0.00
L-Alanine  is an α-amino acid which is used in the biosynthesis of proteins. Immobilized L-Alanine is prepared by covalently coupling amiono group of L-Alanine to the preactivated 6% cross-linked agarose. Click here to request bulk or custom sizes.   Features: L-Alanine c..
$0.00
L-Arginine  is an α-amino acid  which is used in the biosynthesis of proteins. Immobilized L-Arginine is prepared by covalently coupling amiono group of L-Arginine to the preactivated 6% cross-linked agarose. Click here to request bulk or custom sizes.   Features: L-Argin..
$0.00
L-Asparagine is an α-amino acid which is used in the biosynthesis of proteins. Immobilized L-Asparagine is prepared by covalently coupling amiono group of L-Asparagine to the preactivated 6% cross-linked agarose. Click here to request bulk or custom sizes. Features: L-Asparagi..
$0.00
L-Aspartic acid is an α-amino acid which is used in the biosynthesis of proteins. Immobilized L-Aspartic acid is prepared by covalently coupling amiono group of L-Aspartic acid to the preactivated 6% cross-linked agarose. Click here to request bulk or custom sizes. ​ Features: ..
$0.00
Showing 61 to 72 of 128 (11 Pages)

Benchtop Lab Equipment

Teaching Biotechnology

Connect With Us

Stay up to date with G-Biosciences by signing up for our newsletter.