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Purification & Chromatography

Download the Protein Purification Handbook for a full list of related productsDownload the Purification Resins Handbook for Complete Product List

 

We offer a large selection of products for protein purification and chromatography.

Protein Purification STRATEGY™ consists of numerous fractionation and chromatography techniques including pH fractionation, salt fractionation, hydrophobic chromatography, anionic chromatography, and cationic chromatography to aid researchers in developing suitable purification systems for specific and novel proteins.

Our G-Capsule is an easy-to-use device for the rapid electroelution of nucleic acids and proteins that excises DNA or protein bands and elutes your sample in a final volume of ~30µl with as high as 90% protein recovery.  

We offer many products to aid in affinity chromatography for the separation & purification of affinity tagged proteins & antibodies. Immobilized Protein A is available for the purification of immunoglobulin (ig) molecules and Immobilized Protein G is offered for the purification of antibodies through the constant IG domains. Our nickel chelating resin is an immobilized metal affinity chromatography (IMAC) resin that utilizes nickel for the purification of 6x histidine tagged proteins.

Ampicillin is an inhibitor of bacterial cell wall synthesis which acts by inhibiting the cross-linking of peptidoglycan. It is used in the selection of antibiotic cell hybrids, and has a high rate of action agaisnt many gram negative bacterial strains.   Immobilized Ampicillin is made by covale..
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G-Biosciences immobilized antibiotics are prepared by coupling antibiotic to preactivated 6% cross-linked agarose. The covalent coupling to agarose (resin) is achieved by cross-linking amino or carboxyl group depending upon the antibiotic that is coupled to the resin.   Click here to request bu..
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Apramycin, an aminoglycoside antibiotic, is produced by a strain of Streptomyces tenebrarius. Apramycin is a potent inhibitor of protein synthesis in bacteria in vivo and in vitro.    Immobilized Apramycin  is made by covalently coupling apramycin to 6% cross linked agarose and ..
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Aprotinin is a monomeric globular protein which inhibits several serine proteases including trypsin, chymotrypsin, plasmin and kallikrein. Furthermore, its action on kallikrein leads to inhibition of formation of factorXIIa.The binding of aprotinin to proteases is reversible with dissociation of apr..
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Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=1015 M-1). Biotin and avidin interaction is rapid and once the bond is established it can survive up to 3M guanidine-hydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by d..
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Bacitracin is a peptide antibiotic and shows antibacterial activity against gram-positive bacteria. It inhibits bacterial cells wasll synthesis by inhibiting dephosphorylation of lipid pyrophosphate.   Immobilized Bacitracin is made by covalently coupling bacitracin to 6% cross linked aga..
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Immobilized Biotin Resin is designed for the high affinity chromatography purifications of avidin, streptavidin and Neutravidin protein.  The resin consists of biotin coupled to 6% cross-linked agarose.    Biotin, a 244 Dalton vitamin (Vitamin H) molecule, exhibits an extraordin..
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Cephalexin is a cephalosporin antibiotic which disrupts the synthesis of the peptidoglycan layer of bacterial cell walls. Peptidoglycan synthesis is aided by transpeptidase known as pencillin-binding proteins (PBP). Cephalexin inhibits PBP3 and other penicillin-binding proteins (PBPs).  Immobi..
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​Ceftazidime inhibits enzymes which are responsible for bacterial cell-wall synthesis, mainly penicillin binding protein 3 (PBP3). A broad range of gram-negative bacteria are susptible to ceftazidime including strains resistant to gentamicin and other aminoglycosides. Immobilized ​Ceftazidime ..
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​Cloxacillin antibiotic binds to specific penicillin-binding proteins (PBPs) located inside the bacterial cells wall and inhibits the third amd last stage of bacterial cell wall synthesis. It is used against stphyloccoci that produce beta-lactamase. Immobilized Cloxacillin is made by cov..
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D-Alanine is the non-proteinogenic form of alanine which is used in bacterial cell wall biosynthesis. Immobilized D-Alanine is prepared by covalently coupling amiono group of D-alanine to the preactivated 6% cross-linked agarose. Click here to request bulk or custom sizes.   Features: ..
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G-Biosciences Immobilized D-Galactose is for the purification of lectins, galactosidases and other galactose-, N-acetylgalactosamine- or carbohydrate binding molecules.  Specific applications include the purification of galactosidases, C-type lectins, enterotoxins and cholera toxin.   Th..
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