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Purification & Chromatography

Download the Protein Purification Handbook for a full list of related productsDownload the Purification Resins Handbook for Complete Product List

 

We offer a large selection of products for protein purification and chromatography.

Protein Purification STRATEGY™ consists of numerous fractionation and chromatography techniques including pH fractionation, salt fractionation, hydrophobic chromatography, anionic chromatography, and cationic chromatography to aid researchers in developing suitable purification systems for specific and novel proteins.

Our G-Capsule is an easy-to-use device for the rapid electroelution of nucleic acids and proteins that excises DNA or protein bands and elutes your sample in a final volume of ~30µl with as high as 90% protein recovery.  

We offer many products to aid in affinity chromatography for the separation & purification of affinity tagged proteins & antibodies. Immobilized Protein A is available for the purification of immunoglobulin (ig) molecules and Immobilized Protein G is offered for the purification of antibodies through the constant IG domains. Our nickel chelating resin is an immobilized metal affinity chromatography (IMAC) resin that utilizes nickel for the purification of 6x histidine tagged proteins.

Neomycin is a bactericidal aminoglycoside antibiotic. It binds to the 30S ribosome of susceptible organisms and interferes with mRNA binding and acceptor tRNA sites and results in the production of non-functional or toxic peptides. Immobilized Neomycin is prepared by covalently coupling neomycin to..
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G-Biosciences' Immobilized p-Aminophenyl Phosphoryl Choline consists of a phosphoryl choline covalently linked to beaded agarose and is designed for the purification of C-reactive protein from plasma, ascites and other biological fluids.   CRP, C-reactive protein, is a pentameric protein&..
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Penicillin G is a naturally occurring beta lactam antibiotic produced by the Penicillium fungus. Penicillin G works by inhibiting cell wall production in non-resistant cells by breaking the links between peptidogylocans and causing the production of cell wall damaging autolysins. Immobilized ..
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G-Biosciences Immobilized Pepstatin is for the purification of cathepsins, pepsin, bacterial aspartic proteases, HIV proteases and other molecules that bind pepstatin.    Pepstatin is isovaleryl-Val-Val-AHMHA-Ala-AHMHA where AHMHA= (3S, 4S)-4-amino-3-hydroxy-6-methyl-heptanoic acid and i..
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Immobilized Procainamide is designed for affinity purification of butyrylcholinesterase (BuChE), which is also known as non-specific cholinesterase. Butyrylcholinesterase can be purified by batch adsorption on immobilized Procainamide followed by ion-exchange chromatography on DEAE-Sepharose column..
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Model:
Immobilized Protein A is for binding the constant domains of immunoglobulin (Ig) molecules (see Figure 1). Protein A is coupled to agarose beads by reductive amination method that provides high coupling efficiency for immunoglobulins and minimal protein A leaching (<5ng protein A/ml). Our im..
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Immobilized protein G is for binding the constant domains of immunoglobulin (Ig) molecules (see Figure 1). It is ideal for the easy one-step purification of classes, subclasses and fragments of immunoglobulins for biological fluids and cell culture media. Protein G is a modified form of Streptococca..
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Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10¹âµM-¹) and streptavidin (Ka=10¹âµM-¹). Biotin and (strept)avidin interaction is rapid. Once the bond is established, it can survive up to 3M guanid..
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Streptomycin Sulfate is an aminoglycoside antibiotic and has a bactericidal action against many gram-negative bacteria. It binds to the S12 protein on the ribosomal 30S subunit and inhibits protein synthesis    Immobilized Streptomycin is made by covalentlly coupling streptomycin to..
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Sulfadimidine is a bacteriostatic antimicrobial sulfur drug. It blocks the synthesis of dihydrofolic acid by inhibiting the enzyme dihydropteroate synthase. Sulfadimidine is a competitive inhibitor of bacterial para-aminobenzoic acid (PABA), which is required for bacterial synthesis of folic acid. &..
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Tetracycline is a bacteriostatic antibiotic with activity against both gram-positive and gram-negative bacteria. Within the cell tetracycline binds reversibly to the 30S subunit of the ribosome, preventing the binding of aminoacyl tRNA and inhibiting protein synthesis and hence cell growth.   ..
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MES SDS Buffer Kit is a complete kit to resolve small- to medium-sized proteins under denaturing conditions on Bis-Tris gels.   Features MES SDS Buffer Kit includes MES SDS Running Buffer [20X], DTT [0.5 M,10X], Protein Antioxidant [200X] and LDS Sample Loading Buffer [4X] The kit is comp..
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