UPPA-PROTEIN-Concentrate™ (5 Citations)
Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.
The UPPA-PROTEIN Concentrate™ kit uses our proprietary reagent UPPA™ (Universal Protein Precipitation Agent) to quantitatively concentrate dilute protein samples as low as 1ng/ml into a small volume. Protein precipitation and concentration are not affected by the presence of detergents, chaotropics, or other common laboratory agents.
After precipitation, the precipitate is washed to remove salts and other interfering agentscomplete recovery of sample is produced. The resulting protein samples have a conductivity of about 50µS and protein recovery is generally 100%.
The UPPA-PROTEIN Concentrate™ kit is available in two sizes: a Micro Kit for concentrating up to 10ml of dilute protein solutions and a Medi Kit for concentrating up to 30ml of dilute protein solutions, either as a single or multiple procedures.
Features
- Concentrate as dilute as 1ng/ml
- Removes non-protein agents
- Low conductivity: ~50µS
- 100% Sample Recovery
Applications
- Concentrate dilute protein samples
- Suitable for gel electrophoresis, raising antibodies, protein purification, protein assays & more
Protocol | |
786-120 | |
786-121 |
Material Safety Data Sheet | |
786-120 | |
786-121 |
Technical Literature | |
Concentration of Dilute Protein Solutions | |
Mass Spectrometry Sample Prep Handbook | A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation. |
Plant Proteomics Handbook | |
Protein Electrophoresis Handbook | A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products. |
Protein Labeling & Conjugation | |
Sample Preparation Handbook | For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment |
- Wan, C. et al (2015) Proteome-wide dataset supporting the study of ancient metazoan macromolecular complexes. Data Brief. doi:10.1016/j.dib.2015.11.062
- McFadden, N. et al (2011) PLoS Pathog 7(12): e1002413
- Scherp, P. et al (2011) Methds Mol. Biol. 702:163
- Sihlbom, C. et al (2008) J. Proteome Res. 7:4191
- Grote, J. et al (2006) BMC Mol. Biol. 7:48