Tube-O-DIALYZER™ for Laboratory Dialysis (168 Citations)

Tube-O-DIALYZER™ is a unique laboratory dialysis device for small scale dialysis, 0.02-2.5ml, that allows for total sample recovery, even if protein precipitation occurs.  The proprietary design is based on standard laboratory centrifuge tubes that allow common reactions, such as labeling and cross-linking, to be performed and then unwanted reagents removed by small-scale dialysis, all in the same tube.  Once dialysis is complete the Tube-O-DIALYZER™ can be centrifuged to recover 100% of the sample, even if the sample precipitates. Supplied with five different molecular weight cut-off (MWCO) dialysis membranes (1,000, 4,000, 8,000, 15,000 and 50,000 daltons). The disposable Tube-O-DIALYZER™ mini dialysis device system is designed for small sample volumes of 20µl to 2.5ml. Both sizes are available with membranes with molecular weight cutoffs (MWCO) of 1k, 4k, 8k, 15k, and 50k Daltons.

Dialysis is a popular technique used for the exchange of buffer medium across semi-permeable membranes. Dialysis devices are available in many configurations for research applications. We offer innovative dialysis devices and accessories for processing small samples. Small scale dialysis has become a routine technique in life science research. Today's major concern with dialysis devices is the loss of precious samples, due to leaking or precipitation of samples during dialysis. One common cause of sample loss is the accidental piercing of the fragile dialysis membrane with a sharp object, such as a needle, often used to load slide dialysis cassettes, or the breaking or unknotting of dialysis bags.. A second concern is the efficiency and rate of the dialysis. The unique, patented tube design of Tube-O-DIALYZER™ permits easy sample handling and manipulation, without the use of specialized loading devices, costly syringes or hazardous needles. This specialized tube format also allows for easy sample loading, as simple as transferring your sample to a centrifuge tube with a pipette. For sample recovery, centrifuge for five seconds to collect 100% of your sample. and the store the dialyzed sample by simply replacing the dialysis cap with the supplied storage cap.

The Tube-O-DIALYZER™ tube comes in two sizes; the Micro unit allows efficient dialysis of 20-250µl samples and the Medi unit is optimized for samples between 0.2-2.5ml. Supplied in packs of 20, each supplied with 6 appropriately sized floats and storage caps.  For added convenience, Tube-O-DIALYZER™ is also available as a mixed kit containing 10 Micro and 10 Medi units.

A graph representing the fast and highly efficient dialysis rate of the micro Tube-O-DIALYZER™ is shown above. 100µl 5M NaCl was dialyzed against one liter deionized water. Samples were taken at specific times, diluted in deionized water and the conductivity was measured. The graph demonstrates the fast efficiency of Tube-O-DIALYZER™, with 50% NaCl removed within 10 minutes.

Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community. 

Features

• Micro for 20-250µl samples, Medi for 0.2-2.5ml samples
• 100% sample recovery every time, even if sample precipitates
• No use of specialized loading devices, costly syringes or hazardous needles, no knotting required
• Storage caps provided for convenient storage of dialyzed samples

Applications

• Dialysis of small sample volumes
• Equilibrium dialysis for buffer exchange
• Dialysis for single use applications, such as infectious and radioactive samples
• Concentration of samples when used with our Concentrator Powder (Cat. No. 786-144)
• For more information on preparing protein samples, view our Sample Preparation Handbook: a handbook and selection guide for lysis buffers and systems, protein fractionation kits, dialysis systems, electrophoresis clean-up and concentration systems, and contamination removal kits.

Technicals