menu
Your Cart

Sputo-LR™

Catalog Description Size Price(USD) Qty
786-1086
Sputo-LR™ 10 ml
$41.00
786-1087
Sputo-LR™ 20 x 10ml vials
$247.00

Sputo-LR™

Sputo-LR™ is a sputum liquefying reagent for sputum and other mucoid fluids that is useful for the isolation of pathogenic or saprophytic bacteria, fungi, yeast, and epithelial cells. Sputo-LR™ is a concentrated sterile solution of Cleland’s Reagent (dithiothreitol, DTT) in phosphate buffer at pH 7.0 and is for processing samples from sputum for smears and culture.

 

Sputum or phlegm is the discharge that is expectorated from the respiratory system. It is a heterogeneous and viscous substance that differs in appearance (mucoid, purulent, mucopurulent and blood-streaked). Consequently, the various host components and organisms in the sputum are not evenly distributed which makes it difficult to process multiple, equivalent samples for research and cryopreservation.

 

Sputo-LR™ chemically digests sputum to decrease viscosity and create a homogenous suspension that allows each sample to be an equivalent representation of the original sputum specimen. This chemical digestion or sputum liquefaction is accomplished with DTT. The active component of DTT is a sulphydryl group, which reduces the mucoprotein cross-linking disulfide bonds. A small amount of DTT is added to the specimen, so it does not affect morphology, growth or fluorescent antibody staining of pathogens in sputum.

Protocol
786-1086
786-1087
Material Safety Data Sheet
786-1086
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Finnish
Swedish
Polish
786-1087
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Finnish
Swedish
Polish
Write a review
Note: HTML is not translated!
Bad Good
Captcha

Download our handbooks

Custom Services

Connect With Us

Stay up to date with G-Biosciences by signing up for our newsletter.