Sample Preparation
Sample preparation is crucial to an accurate and informative downstream analysis. Frequently, the component of interest is present at levels too low for detection or in the presence of interfering matrix elements that may mask the analyte. Properly selected sample preparation techniques can remove excess contaminants and concentrate the analyte to adequate levels for measurement. Applied properly these techniques yield clean and informative samples. G-Biosciences offer a variety of tools designed to remove interfering contaminants and concentrate your samples to desirable concentrations.
Dialysis techniques are reliable and cost effective methods for concentrating samples. G-Biosciences created novel dialysis tools which allow for the concentration of samples with zero sample loss. Tube-O-CONCENTRATOR™ is a versatile concentration tool which utilizes a novel, water-absorbing, liquid polymer and the patented Tube-O-DIALYZER™ for rapid concentration of dilute sample solutions with zero analyte loss. The Tube-O-CONCENTRATOR™ and Tube-O-DIALYZER™ systems are designed for centrifugation. The Tube-O-REACTOR™ is a system that allows key steps of cross-linking, coupling, and modification of proteins and/or nucleic acids for be performed in a single tube. This tool minimizes the risk of sample loss and reduces experimental time and hands-on steps.
Removal of interfering agents such as detergents, salts, dyes, and radioisotopes in an integral part of effective sample preparation. For these purposes, G-Biosciences developed an array of spin columns which accomodate a variety of volumes and molecular weight ranges. The SpinOUT™ GT-100, SpinOUT™ GT-600, and SpinOUT™ GT-1200 columns are versatile, spin-format columns for the desalting and buffer exchange of peptide, protein, and nucleic acid samples ranging from 5ųL to 4mL sample volumes. This technology is also available in a 96-well spin plate format. Optimized for FPLC applications, the G-Trap™ line of desalting columns are ideal for buffer exchange and desalting of samples of a variety of volumes and MWCO’s. G-Trap™ columns are available for three MWCO’s, 700Da G-Trap™ GT-100, 6kDa G-Trap™ GT-600, and 30kDa G-Trap™ GT-1200.
Preciptation of proteins is a common purification technique. OrgoSol-PROTEIN-Concentrate™ precipitates protein without disrupting the native structure, retaining the activity of most proteins. A Universal Protein Precipitation Agent (UPPA™) was developed for the concentration of protein solutions as dilute as 1ng/mL. Protein precipitation is not affected by detergents, chaotropic agents, or other reagents when using the UPPA™ system and recovery is generally 100%.
