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Proteomics

Our Protein Research section covers the spectrum of research products for proteomics.

There are products for Protein Purification, including Protein Extraction, Fractionation, Sample Preparation and Protein Concentration.

Our Protein Analysis products cover Protein Electrophoresis, Western Blot, and Mass Spectrometry.  A wide selection of Protein Labeling and Protein Modification tools are available for protein binding and protein structure studies.

A large selection of Protein Assays are available, including unique Protein Estimation Assays.

Many commercial grade detergents contain elevated levels of sulfhydryl oxidizing agents, peroxides, salts and carbonyl compounds (Figure 1). The proteins that are isolated with these detergents are highly susceptible to contaminating peroxides and carbonyls. The peroxides will oxidize proteins and t..
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Gene/Protein:
A suitable protein solubilization buffer for 2D analysis must solubilize proteins effectively, without disturbing the native charge of the proteins. Urea based solubilization buffers solubilize proteins effectively, however can modify the native charge of the proteins, due to carbamylation. Ur..
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ρ-Azidobenzoyl Hydrazide Features Molecular Weight: 177.19 Spacer Arm (Å): 11.9 Reactive Toward: Carbohydrates Membrane Permeable: YES Water Soluble: NO Cleavable/ Reversible: NO                       ..
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ActiveHOOK™ BSA is maleimide activated BSA, by the addition of sulfoSMCC cross-linker. The ActiveHOOK™ BSA rapidly couples to free sulfhydryl groups on peptides and proteins. This activated carrier proteins save both time and money as separate cross-linkers are not required and the ..
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ActiveHOOK™ HyperCarrier™ is maleimide activated HyperCarrier™, by the addition of sulfoSMCC cross-linker. The ActiveHOOK™ HyperCarrier™ rapidly couples to free sulfhydryl groups on peptides and proteins. This activated carrier proteins save both time and mone..
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ActiveHOOK™ KLH is maleimide activated by the addition of sulfoSMCC cross-linker. The ActiveHOOK™ KLH rapidly couples to free sulfhydryl groups on peptides and proteins. This activated carrier proteins save both time and money as separate cross-linkers are not required and the ..
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Gene/Protein:
Simply heat and use for sealing the IPG strips for SDS-PAGE analysis. The Agarose Sealing Solution is prepared in a proprietary buffer to minimize reoxidation of the competing thiol pairs as proteins enter into the second dimension gel. Improves resolution and prevents streaking of protein spots on ..
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Gene/Protein:
Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophoresis and other studies. AlbuminOUT™ has been specifically developed for substantial removal of ..
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Gene/Protein:
  Features       CAS#: 631-61-8 Molecular Formula: C2H3O2NH4 Ammonium Acetate Molecular Weight: 77.08    ..
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N-5-Azido-2-nitrobenzyloxysuccinimide    Features Molecular Weight: 305.20 Spacer Arm (Å): 7.7 Reactive Toward: Primary Amines Membrane Permeable: YES Water Soluble: NO Cleavable/ Reversible: No..
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APDP A sulfhydryl-reactive and photoreactive cross-linker   Features Chemical Name: N-[4-(p-Azidosalicylamido)butyl]-3’-(2’-pyridyldithio)propionamide Synonym: APDP Formula: C₁₈H₂₀N₆O₂ Molecular Weight: 446.55 Spacer Arm: 21.0Å Reactive Toward: sulfhydryl and amino..
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ρ-Azidophenyl glyoxal monohydrate   Features Molecular Weight: 193.16 Spacer Arm (Å): 9.3 Reactive Toward: Arginines Membrane Permeable: YES Water Soluble: NO Cleavable/ Reversible: NO                     ..
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Type: Zwitterionic detergent   Full Name: Amidosulfobetaine-14   Molecular Formula: C22H46N2O4S   Molecular Weight: 434.7   Form: White to off white powder   Purity: >99%   Solubility: Water soluble   Conductivity: <50µS in a 10% solu..
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Type: Zwitterionic detergent   Full Name: 4-n-Octylbenzoylamido-propyl-dimethylammonio sulfobetaine   Molecular Formula: C23H40N2O4S   Molecular Weight: 440.6   Form: Off white powder   Purity: >99%    Solubility: Water soluble   Applications..
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Gene/Protein:
Avidin is a glycoprotein with approximately 10% of its total mass comes from carbohydrates. Avidin has a molecular weight of 67kDa and contains four identical 128 amino acid subunits that each have a single biotin binding domain. Avidin is a basic protein with an isoelectric pH of 10-10.5 and is rea..
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Gene/Protein:
Bacterial PE LB™ has been developed for the extraction of soluble proteins and inclusion bodies from bacterial cells. It is a proprietary improvement on the lysozyme based lysis, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA & RNA) released during..
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Bis(β-[4-azidosalicylamido]-ethyl) disulfide   Iodinatable     Features Molecular Weight: 474.52 Spacer Arm (Å): 21.3 Reactive Toward: Non Selective Membrane Permeable: YES Water Soluble: NO Cleavable/ Reversible: Reducing Agents (Thiols) &nb..
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Type: Non-ionic detergent   Full Name: N,N-Bis[3-(D-gluconamido)propyl]cholamide   Molecular Formula: C42H75N3O16   Molecular Weight: 878.1   Form: Fine colorless crystals   Purity: >99%   Solubility: Water soluble   Conductivity: <25µS in..
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Gene/Protein:
Convenient ready-to-use blocking solution for Western blots, dot Blots & ELISA   Features 5% novel modified milk protein solution Use for Westerns, ELISA, and dot Blot blocking Blocking in 10-15 minutes Ready-to-use      ..
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Gene/Protein:
Protein solutions are used for blocking excess binding sites in ELISA, blotting, immunohistochemistry and other immunochemical applications. Each blocking solution will impart certain characteristics on the assay system.     Our BLOK™ BSA is a 10% solution of BSA (Bovine Serum ..
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Gene/Protein:
BLOK™ Casein is a convenient ready-to-use solution of purified casein blocking solutions for Western blots, dot blots, ELISA, IHC and nucleic acid detection methods. The BLOK™ Casein are avaialble as a 1% (w/v) casein,which is the optional concentration for most applications, in 1X ..
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Gene/Protein:
A unique stain for reversibly staining protein on nitrocellulose and PVDF transfer membranes.   BLOT-FastStain™ only stains protein and leaves the background absolutely untouched and brilliant white resulting in high band visibility. The staining procedure takes 10 minutes and has a..
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The blot is prepared using total protein extracted from a variety of 5-10 day old, whole plant tissues.    The premade blots are prepared with the appropriate GenLysate and 50μg protein is loaded onto a 4-20% denaturing polyacrylamide gel, along with a prestained molecular weight mark..
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Gene/Protein:
Bovine serum albumin is a single polypeptide of 67kDa and consists of 59 lysine residues, of which 30-35 have primary amines that can react with crosslinkers for the coupling of peptides.   OneQuant™ BSA The BSA carrier protein is also available as our single use OneQuant™ ..
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Brij® 35 Brij® 35 is a non-ionic detergent that is provided for research purposes or as a 10% proteomic grade solution. Proteomic Grade: DG003, DG004, DG503, DG515 Research Grade: 786-521, 786-351   Proteomic Grade Detergent Solutions Many commercial grade detergents contai..
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Brij® 58 Brij® 58 is a non-ionic detergent that is provided for research purposes or as a 10% proteomic grade solution. Proteomic Grade: DG005, DG006, DG505, DG516 Research Grade: 786-522, 786-352   Proteomic Grade Detergent Solutions Many commercial grade detergents contain..
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(Bis(2-[Succinimidooxycarbonyloxy]ethyl) sulfone   Features Molecular Weight: 436.35 Spacer Arm (Å): 13 Reactive Toward: Primary Amines Membrane Permeable: YES Water Soluble: NO Cleavable/ Reversible: Base    ..
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Calmodulin, Recombinant Human is a full length (Met1- lys149, Accession # P62158) human recombinant calmodulin protein expressed in E. coli. Calmodulin is a ubiquitous calcium- binding protein present in all eukaryotic cells and some cell organelles. Calmodulin plays a pivotal role in calcium signa..
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Gene/Protein:
An improved Coomassie Dye based protein assay based on the Bradford Protein Assay (1). This assay is suitable for the simple and rapid estimation of protein concentration. This assay is based on a single Coomassie dye based reagent. The binding of protein to the dye results in a change of color from..
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Gene/Protein:
The centrifuge adapter is designed for centrifugation of Medi and Micro Tube-O-DIALYZER™ in a bench top centrifuge. The centrifuge adapter is required for 100% sample recovery by centrifugation of the Tube-O-DIALYZER™ system...
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Type: Zwitterionic detergent   Full Name: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate   Mol. Formula: C32H58N2O7S   Mol Weight: 614.9   Form: White solid   Purity: >99%   Solubility: Water soluble   Conductivity: <25µS..
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Type: Zwitterionic detergent   Full Name: 3-[(3-Cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate   Molecular Formula: C32H58N2O8S   Molecular Weight: 630.9   Form: White solid   Purity: >99%   Solubility: Water soluble   Conductivity: ..
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The Column-PROTEIN-Concentrate™ kit has been specifically developed for concentration of those proteins that cannot be concentrated either by precipitation or other techniques. The kit is based on a proprietary Protein Binding Resin that binds and immobilizes any protein in a low salt buffer b..
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Gene/Protein:
Concentrator Powder is a high molecular weight polymer which will not migrate across the dialysis membrane.  Simply transfer your dilute protein solution to a dialysis bag or dialysis device, such as our patented Tube-O-DIALYZER™ and then cover the membrane with Concentrator Powder. ..
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Gene/Protein:
Concentrator Solution is a novel liquid polymer for the rapid concentration of dilute protein solutions with zero loss, using dialysis.   Simply transfer your dilute protein solution to a dialysis bag or dialysis device*, such as our patented Tube-O-DIALYZER™ and dialyze against the..
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Gene/Protein:
Coomassie Brilliant Blue Dye R-250/ G-250 are both offered as dry powder form and convenient ready-to-use solutions. Destaining solutions are also offered.   Coomassie Brilliant Blue G-250 Dye Proteomics Grade Cas #: 6104-58-1 Molecular weight: 854.04 Molecular Formula: C..
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Gene/Protein:
Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath et al (1), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.  The Copper Chelating Resin is specifically designed for the pu..
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Gene/Protein:
Type: Cationic detergent   Full Name: Hexadecyltrimethylammonium bromide   Mol. Formula: CH3(CH2)15N(Br)(CH3)3   Mol Weight: 364.5   Form: White to off white powder   Purity: >99%   Solubility: Water soluble   Critical m..
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We offer a custom blot service.  Simply select the lysates of interest from our extensive range of GenLysate™ and contact our customer support for ordering and pricing information...
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Type: Non-ionic detergent   Full Name: N,N-Bis[3-(D-gluconamido)propyl]deoxycholamide   Molecular Formula: C42H75N3O15   Molecular Weight: 862.1   Form: White powder   Purity: >95%   Solubility: Water soluble   Critical micelle concentration (CMC):..
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Type: Anionic detergent   Molecular Formula: C24H39NaO4   Molecular Weight: 414.6   Form: White to off white powder   Purity: >99%   Solubility: Water soluble   Critical micelle concentration (CMC): 4-8mM (25°C)   Aggregation number: 3-12 ..
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DeSalting Columns are ready-to-use disposable columns for separating macromolecules, i.e. proteins, from low molecular weight buffer salts and reagents. See our SpinOUT™ product line for rapid desalting of proteins >6 or >30kDa...
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Dialysis is the process of separating molecules in solution by the difference in their rates of diffusion through a semi permeable membrane, such as dialysis tubing or Tube-O-DIALYZER™ dialysis caps. Molecules small enough to pass through the dialysis membrane move across the membrane in ..
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1,4-Di-(3’-[2’pyridyldithio]-propionamido) butane   Features Molecular Weight: 428.71 Spacer Arm (Å): 19.9 Reactive Toward: Sulfhydryls Membrane Permeable: not done  Water Soluble: NO Cleavable/ Reversible: Reducing Agents (Thiols)    ..
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Dithiobis(succinimidyl propionate)   Features Molecular Weight: 404.42 Spacer Arm (Å): 12 Reactive Toward: Primary Amines Membrane Permeable: YES Water Soluble: NO Cleavable/ Reversible: Reducing Agents (Thiols)    ..
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Disuccinimidyl suberate   Ideal for receptor ligand cross-linking and coupling antibody to Protein A or G for immunoprecipitations.   OneQuant™ DSS is supplied as 8 vials of 2mg DSS, suitable for preparing for ~100µl 50mM stock solution from each vial.   Features ..
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Disuccinimidyl tartrate   Features Molecular Weight: 344.24 Spacer Arm (Å): 6.4 Reactive Toward: Primary Amines Membrane Permeable: YES Water Soluble: NO Cleavable/ Reversible: Oxidizing Agents (Periodate)    ..
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3,3’-Dithiobis(sulfosuccinimidyl propionate)   Features Molecular Weight: 608.51 Spacer Arm (Å): 12 Reactive Toward: Primary Amines Membrane Permeable: NO Water Soluble: YES Cleavable/ Reversible: Reducing Agents (Thiols)    ..
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Gene/Protein:
EDC is a heterobifunctional, water-soluble, zero-length carbodiimide crosslinker that is used to couple carboxyl groups to primary amines. EDC activates carboxyl groups first and forms amine reactive O-acylisourea imtermediate that spontaneously reacts with primary amines to form an amide bond and i..
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Gene/Protein:
G-Biosciences scientists have developed a high efficiency transfer buffer for Western blotting. The pre-made and ready-to-use [20X] transfer buffer is prepared for optimal conductivity and efficient protein transfer without generating excessive heat or transfer distortion. Efficient™ Western T..
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Ethylene glycol bis(succinimidyl succinate)   Ideal for receptor ligand crosslinking   Features Molecular Weight: 456.36 Spacer Arm (Å): 16.1 Reactive Toward: Primary Amines Membrane Permeable: YES Water Soluble: NO Cleavable/ Reversible: Hydroxylamine   ..
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Gene/Protein:
5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB)   Ellman's reagent is a versatile, water-soluble compound for quantifying free sulfhydryl groups in solution. It reacts with a free sulfhydryl group to yield a mixed disulfide and 2-nitro-5-thiobenzoic acid (NTB), a measurable yellow co..
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N-(ε-Maleimidocaproic acid) hydrazide   Features Molecular Weight: 225.24 Spacer Arm (Å): 11.8 Reactive Toward: Sulfhydryl + Carbohydrate Membrane Permeable: Not tested  Water Soluble: NO Cleavable/ Reversible: NO    ..
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N-(ε-Maleimidocaproyloxy) succinimide ester   Features Molecular Weight: 308.29 Spacer Arm (Å): 9.4 Reactive Toward: Primary Amine + Sulfhydryl Membrane Permeable: YES Water Soluble: NO Cleavable/ Reversible: NO    ..
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Enhancer™ allows for the rapid application of up to 384 protein or nucleic acid samples on to a 12 x 9cm membrane for subsequent analysis. Samples are applied to a nitrocellulose or nylon membrane by capillary action, resulting in concentration of sample due to minimal diffusion. Enhancer&tr..
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Gene/Protein:
Add an appropriate volume of the supplied rehydration buffer to the dry, ready-to-use, urea based equilibration buffer.   Eliminates worries about urea induced protein carbamylation or waste of unused reagents, as fresh and ready-to-use reagents are prepared every time.   The E..
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Gene/Protein:
A highly efficient grinding resin that is pre-aliquoted into 1.5ml grinding tubes and is supplied with matching pestles. The resin is designed for optimal grinding of biological samples for the extraction of both proteins and DNA.   The resin is a neutral abrasive material that does not b..
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Gene/Protein:
FASTSilver™ is one of the most rapid and sensitive methods for detecting proteins and nucleic acids fractionated by PAGE. Staining is 100 times more sensitive than Coomassie Blue protein staining and 10 times more sensitive than ethidium bromide for DNA and RNA. FASTsilver™ is a nanog..
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Gene/Protein:
femto TBST™ and femto PBST™ will enhance sensitivity of your immunoassays by minimizing the "washing out" of immuno agents or immuno complexes, a common problem associated with classical TBST and PBST buffers. The wash buffers aid in the generation of cleaner backgrounds, resul..
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femtoCHROMO™ AP kit is supplied with a single step chromogenic substrate for the rapid development of protein bands with low background staining. To ensure low background staining, each kit is supplied with an enhanced blocking agent, BLOT-QuickBlocker™, and a concentrated (10X) was..
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femtoCHROMO™-HRP kit is supplied with a single step chromogenic substrate for the rapid development of protein bands with low background staining.  To ensure low background staining, each kit is supplied with an enhanced blocking agent, BLOT-QuickBlocker™, and a concentrated (10X) w..
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Gene/Protein:
Enzyme-Linked Immunosorbent Assay (ELISA) is a useful, highly sensitive and powerful technique for the detection of proteins, chemicals, and drugs (antigens) in biological samples, including serum, blood and urine. The key to an ELISA is the interaction of a known antibody with the antigen of intere..
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Gene/Protein:
FirstChoice™ Blocking Buffers contain a proprietary protein formulation that offers greater versatility and lack of cross-reactivity.  This makes FirstChoice™ Blocking Buffers ideal as a first choice for optimization of new assays, system or when determining the optimal blocking buf..
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Gene/Protein:
FISH-Blocker is a blocking agent that uses a fish protein as the primary blocking agent.  The use of a fish protein, a non-mammalian protein, is that it eliminates or minimizes the interaction of antibodies raised in mammals.  FISH-Blocker is one of the best blocking agents for immunoassay..
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Replacement Tube-O-DIALYZER™ floats are also available separately.  Floats for Tube-O-DIALYZER™ Micro and Medi sizes are available.  The floats for Micro are available in two sizes: 786-141F is designed for dialysis in Tube-O-Tanks or a beaker and 786-149 is designed for dialys..
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Gene/Protein:
The Fluoro™ Protease Assay Kit is designed for the quantitative determination of proteases present in a protein sample. The assay uses fluorescein isothiocyanate (FITC)-labeled casein as a general protease substrate. The fluorescein label on the FITC-casein is highly quenched. When the proteas..
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Specifically designed for bacterial research and supplied with bacteria specific reagents. Extracts and solubilizes nearly all of the proteins from E.coli, including membrane as well as soluble proteins.  Extraction is based on the gentle lysis of bacterial cells with LongLife™ Lysozyme e..
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Gene/Protein:
The FOCUS™ Cytoplasmic and Nuclear Protein Fractionation Kit is supplied with a strong chaotropic extraction buffer to solubilize both cytoplasmic and nuclear proteins, which is fully compatible with 2D gel electrophoresis . The FOCUS™ Cytoplasmic and Nuclear Protein Fractionatio..
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Gene/Protein:
For those who prefer DTT as a reducing agent, we offer ultra pure proteomic grade DTT for protein reduction. FOCUS™ DTT is supplied as 5 vials containing dry powder to maintain the activity of the reducing agent. Reconstitute in 0.8ml proteomic grade water for a final volume of 1ml at 2M ..
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Chaotropic extraction buffers that preserve the native charge of proteins making them suitable for sample extraction for 2D gels   One of the most important considerations before running 2D gel electrophoresis is the choice of protein solubilization buffers. A suitable buffer must solubilize ..
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Gene/Protein:
FOCUS™ FASTsilver™ produces crystal clear backgrounds and maximal peptide recovery needed for critical analysis by mass spectrometry.   For mass spectrometry analysis, complete proteolytic digestion and recovery of peptides is required for optimal analysis, however silver ions..
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FOCUS™ Global Fractionation kit is designed to fractionate complex biological samples into cytosolic and membrane fractions. The resulting membrane fractions are subsequently fractionated into either peripheral and integral membrane proteins or lipid raft associated proteins and detergent solu..
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FOCUS™ Glycoprotein kit is based on lectin binding of specific glycoproteins with terminal α-D mannosyl and α-D glycosyl proteins.   FOCUS™ Glycoprotein kit isolates glycoproteins from complex biological solutions using spin columns that contain lectin (Concanavalin A)..
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Extracts and solubilizes nearly all of the proteins from insect cell cultures (i.e. Sf9 and Sf21), including membrane as well as soluble proteins, using a strong chaotropic extraction buffer to solubilize even the most difficult proteins.     Features Single step extraction protoco..
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