Our Boronate resin is designed for the isolation of ribonucleotide and oligonucleotide RNA.
The resin consists of boronic acid covalently linked to a polyacrylamide support that offers simple isolation of small molecular weight compounds that have cis-diol groups.
Mechanism: The boronic acid interacts with the cis-diol groups, found in the sugar portion of nucleotides, forming a reversible five member ring complex. Impurities are washed away and then the complex dissociated by low pH or presence of sorbitol.
The polyacrylamide support excludes >6000 Da molecules from entering the resin bed and therefore is suitable only for small molecules.
- Polyacrylamide gel
- >1.2meq/g boronate load capacity
- >130µmol sorbitol/ml gel binding capacity
- 6,000Da exclusion limit
- 45-90µm wet bead size