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Carbohydrate Coupling Resin (Hydrazide Resin)

Hydrazide resin coupling to carbohydrates

The Carbohydrate Coupling Resin (Hydrazide resin) is designed for the simple and efficient coupling of glycoproteins to a solid agarose support through oxidized sugar groups. The coupling is through the hydrazide group that is immobilized to agarose beads. The resin is ideal for immobilized polyclonal antibodies as these are abundant in carbohydrates in their Fc domain. The location in the Fc domain ensures the antibody’s binding site is orientated away from the resin for optimal binding and reduced steric hindrance. 

Glycoprotein sugar components are first oxidized with sodium meta-periodate to convert the cis-glycol groups to reactive aldehydes. These aldehydes react spontaneously with the hydrazide group on the Carbohydrate Coupling Resin, forming stable hydrazone bonds (see figure). The long spacer arm (23Å) reduces steric hindrance and ensures greater binding of proteins and antibodies during affinity purification. The generated affinity resin can be reused at least 10 times with no significant loss of activity.

The Carbohydrate Immobilization kit is supplied with carbohydrate coupling resin columns for the preparation of 2ml affinity columns, a coupling catalyst, SpinOUT™ GT-600 Desalting Columns for removing oxidizing reagents, the oxidizing reagent (Sodium meta-periodate) and relevant buffers.

Click here to request bulk or custom sizes.
 

Features

  • Hydrazide activated resin for binding reactive aldehydes
  • Couple 1-5mg glycoprotein/ml resin
  • Long Spacer Arm (23Å)
  • Gentle coupling conditions

Applications

  • Immobilize glycoproteins
  • Immobilize polyclonal antibodies through their Fc domain for optimal orientation
  • Immobilize any molecule with oxidized sugars
Protocol
786-807
786-808
Material Safety Data Sheet
786-807
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786-808
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
A Catalyst for Improved Coupling of Carbohydrates
Plant Proteomics Handbook
Protein Labeling & Conjugation
Protein Labeling & Conjugation
Protein Purification Handbook

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