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Co-NTA Resin

Catalog Description Size Price(USD) Qty
786-932
Co-NTA Resin 10ml Resin
$104.00
786-933
Co-NTA Resin 100ml Resin
$702.00
786-934
Co-NTA Resin 500ml Resin
$2,585.00
786-935
Co-NTA Resin 2 x 500ml Resin
$4,942.00
786-936
Co-NTA Resin, 0.2ml Spin Column 25 columns
$182.00
786-937
Co-NTA Resin, 1ml Spin Column 5 columns
$119.00
786-938
Co-NTA Resin, 3ml Spin Column 5 columns
$172.00
786-988
Co-NTA Spin Plates 2 plates
$333.00

Co-NTA Resin

Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.  

 

The Co-NTA Resin is specifically designed for the purification of recombinant proteins fused to the 6 x histidine (6XHis) tag expressed in bacteria, insects, and mammalian cells.  The resin is high affinity and selectivity for recombinant fusion proteins that are tagged with six tandem histidine residues.  Although 6X His tagged proteins bind with a lower efficiency compared to nickel chelating resins there is a significant reduction in non-specific binding.

 

The Co-NTA resin can be used to purify 6X His tagged proteins under native and denaturing conditions.  Proteins bound to the resin can be eluted with low pH buffer or competition with imidazole or histidine.

 

The Co-NTA resin uses nitrilotriacetic acid (NTA), a tetradenate chelating ligand, in a highly cross-linked 6% agarose matrix.  The NTA binds Co2+ ions by four coordination sites.

 

This resin has a capacity of 20-40μmoles Co2+/ml resin.  The protein binding capacity is >50mg protein per ml resin.  We have demonstrated binding of >100mg of a 50kDa 6XHis tagged proteins to a ml of resin. 

 

The spin columns are supplied with a resin bed volume of 0.2, 1 and 3ml with total column volumes of 1, 8 and 22ml respectively. Columns can be used as a spin format of gravity flow columns.

 

Also available in 96-well spin plate formats for processing up to 96 samples.

 
Immobilized IDA NickelCopper, Cobalt  and Zinc and Ni-NTA Chelating Resins are also available.  Cobalt has the highest selectivity followed by Zinc, Nickel then Copper, but has the lowest loading capacity.  Copper has the highest loading capacity, followed by Nickel then Zinc.
 
Specific binding/wash and elution buffers are available.
 
Our new HOOK™ 6X His Protein Purification kits are now available and include everything required for purification of 6X His tagged proteins from yeast or bacteria, including lysis buffers, lytic enzymes, resin, columns and binding, wash and elution buffers. Each kit is available with nickel or cobalt chelating resins. Kits available are:
HOOK™ 6X His Protein Purification (Bacteria): Isolation from bacteria; optimized to yield up to 10mg/250ml culture of soluble 6X His tagged protein.
HOOK™ 6X His Protein Spin Purification (Bacteria): Isolation from bacteria;optimized to yield ~1mg/50ml culture of soluble 6X His tagged protein
HOOK™ 6X His Protein Purification (Yeast): Isolation from yeast; optimized to yield up to 10mg of soluble 6X His tagged protein.
HOOK™ 6X His Protein Spin Purification (Yeast): Isolation from yeast; optimized to yield up to 1mg of soluble 6X His tagged protein.
 
 


Features

  • Uses nitrilotriacetic acid (NTA), a tetradenate chelating ligand
  • For the purification of 6x His proteins
  • High capacity: >50mg/ml
  • Ligand density: 20-40μmoles Co2+ /ml resin
  • Bead Structure: 6% cross-linked agarose

 


Applications

  • Affinity purification of proteins with a 6x His tag.

 

 

Protocol
786-932
786-933
786-934
786-935
786-936
786-937
786-938
786-988
Material Safety Data Sheet
786-932
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786-933
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786-934
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786-935
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786-936
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786-937
English_US
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Dutch
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French
German
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Portuguese
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Polish
786-938
English_US
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Dutch
English_UK
French
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Portuguese
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Polish
786-988
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
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