Immobilized metal affinity chromatography (IMAC) resin utilizing nickel (Ni2+) for the purification of 6x histidine tagged proteins.
This resin binds to six histidine residues (6x His), a common tag used in protein purification. The resin consists of iminodiacetate coupled to 6% cross-linked agarose beads. The iminodiacetate binds divalent nickel ion with a capacity of 20-40μmoles Ni2+/ml resin. The protein binding capacity is >50mg protein per ml resin. We have demonstrated binding of >100mg of a 50kDa 6XHis tagged proteins to a ml of resin. A Nickel NTA resin is also offered.
The spin columns are supplied with a resin bed volume of 0.2, 1 and 3ml with total column volumes of 1, 8 and 22ml respectively. Columns can be used as a spin format of gravity flow columns.
Immobilized Copper, Cobalt and Zinc Chelating Resins are also available. Cobalt has the highest selectivity followed by Zinc, Nickel then Copper, but has the lowest binding capacity. Copper has the highest binding capacity, followed by Nickel then Zinc.
Specific binding/wash and elution buffers are available.
Our new HOOK™ 6X His Protein Purification kits are now available and include everything required for purification of 6X His tagged proteins from yeast or bacteria, including lysis buffers, lytic enzymes, resin, columns and binding, wash and elution buffers. Each kit is available with nickel or cobalt chelating resins. Kits available are:
- For the purification of 6x His proteins
- High capacity: >50mg/ml
- Ligand density: 20-40μmoles Ni2+ /ml resin
- Bead Structure: 6% cross-linked agarose
- Affinity purification of proteins with a 6x His tag.