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Affinity Purification Resins and Methods

 

An assortment of affinity purification resins for the separation of both tagged and immunoglobulin molecules. A large selection of affinity coupling chemistries are available to isolate your target molecules including carboxyl containing molecules, nucleic acids, glycoproteins, polyclonal antibodies, oxidized sugars and many more.  Efficient Avidin-Streptavidin and Neutravidin purification is simple and affordable with our immobilized biotin and iminobiotin resins. High affinity biotin tagged protein resins are available along with streptavidin binding and elution buffers and reagents for the quantitation of biotin. Calmodulin resin for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells are available as resin only or in kit form including binding and elution buffers. Immobilized p-Aminophenyl Phosphoryl Choline consists of a phosphoryl choline covalently linked to beaded agarose and is designed for the purification of C-reactive protein from plasma, ascites and other biological fluids. A more economical substitute for Immobilized p-Aminophenyl Phosphoryl Choline, Immobilized O-Phosphorylethanolamine is also available for the purification of C-reactive protein.  A human recomboninant C-reactive protein is also offered. Purify your GST-tagged or His-tagged proteins with GST-tagged or His-tagged protein purification resins or kits.  Isolate proteins of the hemostatic pathways, growth hormones and growth factors, lipoproteins and other physiological markers with immobilized heparin and lectins.  Rapidly purify lectins, galactose-binding molecules, agglutinins, N-acetylgalactosamine-binding, N-acetylglucasomine-binding and carbohydrate binding molecules with our lectin purification reagents. A variety of non-porous, high antibody binding capacity, cost effective magnetic beads are available. Immobilized boronic acid is designed specifically for the simple isolation of small molecular weight compounds that have cis-diol groups such as ribonucleotide and oligonucleotide RNA. 

 
Download the Protein Purification Handbook for a full list of related products
Download the Purification Resins Handbook for Complete Product List 
HOOK™ GST Protein Spin Purification kit allows for the rapid purification of soluble, GST tagged protein from bacterial cultures. The bacteria are first lysed with Bacterial PE LB™ and PE LB™-Lysozyme to release total soluble protein, whilst maintaining the structure and activ..
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HOOK™ GST Protein Spin Purification kit allows for the rapid purification of soluble, GST tagged protein from yeast cultures. The yeast are first lysed with Yeast PE LB™ and LongLife™ Zymolyase® to release total soluble protein, whilst maintaining the structure and activit..
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Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=1015 M-1). Biotin and avidin interaction is rapid and once the bond is established it can survive up to 3M guanidine-hydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by d..
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G-Biosciences Immobilized D-Galactose is for the purification of lectins, galactosidases and other galactose-, N-acetylgalactosamine- or carbohydrate binding molecules.  Specific applications include the purification of galactosidases, C-type lectins, enterotoxins and cholera toxin.   Th..
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G-Biosciences Immobilized Heparin is a ready-to-use purification resin for a wide range of proteins.  The resin consists of 6% cross-linked agarose covalently coupled to heparin through amide bonds.  The coupling chemistry used generates a highly stable purification resin that is stable mo..
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Immobilized Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non-denaturing elution buffers.   The..
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G-Biosciences Immobilized Monomeric Avidin Resin is designed for the simple affinity chromatography purifications of proteins, antibodies and other molecules with a biotin tag.  The resin consists of monomeric subunits of avidin covalently coupled to 6% cross-linked agarose, offering a stable, ..
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Immobilized Procainamide is designed for affinity purification of butyrylcholinesterase (BuChE), which is also known as non-specific cholinesterase. Butyrylcholinesterase can be purified by batch adsorption on immobilized Procainamide followed by ion-exchange chromatography on DEAE-Sepharose column..
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Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10¹âµM-¹) and streptavidin (Ka=10¹âµM-¹). Biotin and (strept)avidin interaction is rapid. Once the bond is established, it can survive up to 3M guanid..
$0.00
Immobilized Trypsin is TPCK Treated Trypsin immobilized on 4% agarose that eliminates the contamination of protein digests by the trypsin.  The immobilized trypsin is readily removed by separating the agarose from the digestion solution.   Trypsin is a serine endopeptidase that specific..
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Lectins are proteins or glycoproteins that have at least one noncatalytic domain that binds specifically and reversibly to monosaccharides or oligosaccharides. Lectins are important as they are found in applications in science, medicine, agriculture, pharmaceutical companies, biotechnology and other..
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Lectins are proteins or glycoproteins that have at least one noncatalytic domain that binds specifically and reversibly to monosaccharides or oligosaccharides. Lectins are important as they are found in applications in science, medicine, agriculture, pharmaceutical companies, biotechnology and other..
$0.00
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