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G-Trap™ DEAE Agarose Fast Flow

Catalog Description Size Price(USD) Qty
786-1012
G-Trap™ DEAE Agarose Fast Flow, 1 ml 5 columns
$136.00
786-1013
G-Trap™ DEAE Agarose Fast Flow, 5 ml 5 columns
$295.00

G-Trap™ DEAE Agarose Fast Flow

G-Trap™ DEAE Agarose Fast Flow is ready to use prepacked column used for separation of proteins and other biomolecules based on charge. The resin in G-Trap™ DEAE Agarose Fast Flow is a weak anion exchanger composed of highly crosslinked 6% agarose beads coupled with diethylaminoethyl (DEAE)  weak anion exchange group. The proteins carrying net negative charge will bind to the resin and protein carrying net positive charge will not bind and will come out in flow through fraction.

 

There are other  G-Trap™ Ion Exchange Agarose Fast Flow Columns available in 1 ml and 5 ml column volume namely G-Trap™ CM Agarose Fast FlowG-Trap™ SP Agarose Fast Flow,  and G-Trap™ Q Agarose Fast Flow. G-Trap™ CM Agarose Fast Flow and G-Trap™ SP Agarose Fast Flow contains cation exchanger resin and G-Trap™ Q Agarose Fast Flow contains anion exchanger resin. G-Trap™ Ion Exchange Selection Kit is available for initial screening for the appropriate ion exchanger to purify target protein.

 

G-Trap™ Ion Exchange Agarose Fast Flow Columns are ready to use prepacked columns for separation of biomolecules on the basis of charge. G-Trap™ Ion Exchange Agarose Fast Flow Columns are used for method scouting, separation or purification of biomolecules, sample concentration and sample cleanup for charged molecules. Biomolecules that carry net charge opposite to that of ion-exchanger bind to that ion exchanger and are thus separated from bulk molecules in the sample.

 

The  properties of the G-Trap™ DEAE Agarose Fast Flow resin and the column are listed below

 

G-Trap™ DEAE Agarose Fast Flow resin

Ion Exchanger: Weak anion exchanger

Matrix: Cross-linked agarose beads, 6%

Bead size: 45-165 µm

Ligand: Diethylaminoethyl

Ionic Capacity: 0.11-0.16mmol (Cl-)/ml

Binding Capacity: 90mg HSA/ml medium

pH stability Working Range: 2-9

pH stability Cleaning-in-Place (CIP): 2-14

Maximum Flow Velocity: 450cm/h

Recommended Flow rate: 1 ml/ min and 5 ml/ min for 1 ml and 5 ml column respectively

Maximum Pressure: 0.3MPa

Exclusion limit(globular proteins): 4 x 106

Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8M urea, 8M guanidine hydrochloride, 70% ethanol

Storage Conditions: 4 to 30°C, 20% Ethanol

 


G-Trap™ DEAE Agarose Fast Flow ​, 1 ml columns

Column Volume: 1 ml

Columns Dimension: 0.7 x 2.5 cm

Column Harware Pressure Limit: 0.5 MPa

Column Hardware: Polypropylene (Biocompatible)

 

G-Trap™ DEAE Agarose Fast Flow ​, 5 ml columns

Column Volume: 5 ml

Columns Dimension: 1.6 x 2.5 cm

Column Harware Pressure Limit: 0.5 MPa

Column Hardware: Polypropylene (Biocompatible)

 

Application(s):

  • G-Trap™ DEAE Agarose Fast Flow is ready to use prepacked column used for separation of proteins and other biomolecules based on charge.  The resin in G-Trap™ DEAE Agarose Fast Flow is a weak anion exchanger where diethylaminoethyl (DEAE) weak anion exchange group is coupled to agarose. The proteins carrying net negative charge will bind to the resin and protein carrying net positive charge will not bind and will come out in flow through fraction.

 

Protocol
786-1012
786-1013
Material Safety Data Sheet
786-1012
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786-1013
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
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    Lide, D. (2002).Handbook of chemistry and physics, 83rdedition, CRC.

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