A broad range, bacterial, 100X concentrated, ready-to-use protease inhibitor cocktail of AEBSF, 1,10-Phenathroline, Benzamidine, Iodoacetamide, Pepstain A and PMSF that offers greater protection for recombinant proteins expressed and purified from bacteria. The protease inhibitor cocktail inhibits bacterial serine, cysteine, metallo- and other bacterial specific proteases including aminopeptidases and aspartic proteases. Recom ProteaseArrest™ cocktail does not use EDTA as its metalloprotease inhibitor as it would inhibit the activity of proteins that require divalent cations (Ca²+, Mg²+ or Mn²+) for their biological activity. In addition, EDTA would inhibit the purification of proteins using immobilized metal affinity chromatography (IMAC), for example His tagged or CBP tagged proteins. Recom ProteaseArrest™ cocktail is compatible with immobilized metal affinity chromatography.
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Sample Type & key features
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Inhibition Specificity
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1X Composition
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Concentration & Available Sizes
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Recom ProteaseARREST™
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For recombinant protein isolation
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Inhibits bacterial serine, cysteine, metallo- and other bacterial specific proteases including aminopeptidases and aspartic proteases
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668µM AEBSF
1mM 1,10-Phenathroline
1mM Benzamidine
1mM Iodoacetamide
1µM Pepstatin A
1mM PMSF
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100X DMSO solution
1ml, 5ml
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Features
- Protease inhibitor cocktail for greater protection of bacterial expressed recombinant proteins
- 100X concentrated, ready-to-use solution.
- No proprietary ingredients, fully disclosed formulation of six broad range protease inhibitors, including AEBSF, 1,10-Phenathroline, Benzamidine, Iodoacetamide, Pepstain A and PMSF
Applications
- Inhibition of protease activity in protein preparations of bacterial lysates.
- Protection of proteins from proteolysis during purification of recombinant proteins.
ProteaseArrest Family
Our ProteaseArrest™ is a highly efficient, liquid format general protease inhibitor cocktail that offers >95% protease inhibition. The ProteaseArrest™ family of inhibitor cocktails includes other species-specific inhibitor cocktails as well, all of which are enhanced with inhibitors specific for that species. In addition to Bacteria, species-specific cocktails are available for Bacterial, Plant, Yeast, Fungus and Mammalian samples. Our FOCUS™ ProteaseArrest™ is designed to protect proteins destined for 2D electrophoresis and mass spectrometry analysis. The TCM ProteaseARREST™ protease inhibitor cocktail is a broad range, 200X concentrated, ready-to-use protease inhibitor cocktail sterile filtered for tissue culture media. TCM ProteaseArrest™ inhibits a wide range of serine, cysteine and other specific proteases including aminopeptidases trypsin-like and acid proteases. Designed for the protection of proteins secreted in to the culture media.
