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Recom ProteaseArrest™ Protease Inhibitor Cocktail

Recombinant Protein Purification Protease Inhibitor Cocktail
Catalog Description Size Price(USD) Qty
786-376
Recom ProteaseArrest™ [100X] 1ml
$66.00
786-436
Recom ProteaseArrest™ [100X] 5ml
$240.00

Recom ProteaseArrest™ Protease Inhibitor Cocktail

A broad range, bacterial, 100X concentrated, ready-to-use protease inhibitor cocktail of AEBSF, 1,10-Phenathroline, Benzamidine, Iodoacetamide, Pepstain A and PMSF that offers greater protection for recombinant proteins expressed and purified from bacteria. The protease inhibitor cocktail inhibits bacterial serine, cysteine, metallo- and other bacterial specific proteases including aminopeptidases and aspartic proteases.​ Recom ProteaseArrest™ cocktail does not use EDTA as its metalloprotease inhibitor as it would inhibit the activity of proteins that require divalent cations (Ca²+, Mg²+ or Mn²+) for their biological activity. In addition, EDTA would inhibit the purification of proteins using immobilized metal affinity chromatography (IMAC), for example His tagged or CBP tagged proteins. Recom ProteaseArrest™ cocktail is compatible with immobilized metal affinity chromatography.

Sample Type & key features

Inhibition Specificity

1X Composition

Concentration & Available Sizes

Recom ProteaseARREST™

For recombinant protein isolation

Inhibits bacterial serine, cysteine, metallo- and other bacterial specific proteases including aminopeptidases and aspartic proteases

668µM AEBSF

1mM 1,10-Phenathroline

1mM Benzamidine

1mM Iodoacetamide

1µM Pepstatin A

1mM PMSF

100X DMSO solution

1ml, 5ml

Features

  • Protease inhibitor cocktail for greater protection of bacterial expressed recombinant proteins
  • 100X concentrated, ready-to-use solution.
  • No proprietary ingredients, fully disclosed formulation of six broad range protease inhibitors, including AEBSF, 1,10-Phenathroline, Benzamidine, Iodoacetamide, Pepstain A and PMSF

Applications

  • Inhibition of protease activity in protein preparations of bacterial lysates.
  • Protection of proteins from proteolysis during purification of recombinant proteins.

ProteaseArrest Family

Our ProteaseArrest™ is a highly efficient, liquid format general protease inhibitor cocktail that offers >95% protease inhibition. The ProteaseArrest™ family of inhibitor cocktails includes other species-specific inhibitor cocktails as well, all of which are enhanced with inhibitors specific for that species.  In addition to Bacteria, species-specific cocktails are available for BacterialPlantYeast, Fungus and Mammalian samples. Our FOCUS™ ProteaseArrest™ is designed to protect proteins destined for 2D electrophoresis and mass spectrometry analysis. The TCM ProteaseARREST™ protease inhibitor cocktail is a broad range, 200X concentrated, ready-to-use protease inhibitor cocktail sterile filtered for tissue culture media. TCM ProteaseArrest™ inhibits a wide range of serine, cysteine and other specific proteases including aminopeptidases trypsin-like and acid proteases. Designed for the protection of proteins secreted in to the culture media.

Protocol
786-376
786-436
Material Safety Data Sheet
786-376
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786-436
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
Protease & Phosphatase Inhibitors & Proteases Handbook  A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays
Protease & Phosphatase Inhibitors & Proteases Handbook  A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays
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