PhosphataseArrest™ Phosphatase Inhibitor Cocktail (44 Citations)

The PhosphataseArrest™ phosphatase inhibitor cocktails are 100X concentrated, ready-to-use solutions that are simply added to your extraction buffers or samples. PhosphataseArrest™ is ideal for inhibition in tissue extractions and cell lysis experiments and is compatible with most common protein assays. In addition, PhosphataseArrest™ III is compatible with IEF/2D studies.

PhosphataseArrest™ I

• A broad spectrum phosphatase inhibitor cocktail consisting of five phosphatase inhibitors that target all the phosphatase categories: serine/threonine (Ser/Thr) specific, tyrosine specific and dual specificity phosphatases
• A stabilized solution of sodium fluoride, sodium orthovanadate, sodium pyrophosphate, ß-glycerophosphate and sodium molybdate

PhosphataseArrest™ II

• A phosphatase inhibitor cocktail consisting of five phosphatase inhibitors that target acid, alkaline and tyrosine phosphatases
• Contains optimized concentrations of sodium fluoride, sodium tartrate, sodium orthovanadate, imidazole and sodium molybdate

PhosphataseArrest™ III

• A phosphatase inhibitor cocktail consisting of three phosphatase inhibitors that target both serine/threonine and alkaline phosphatases
• A stabilized solution of cantharidin, p-bromotetramisole oxalate and microcystin-LR

* If samples have high phosphatase activity then the PhosphataseArrest™ inhibitor cocktails can be used at two to three times concentrations to ensure complete inhibition.

Features

• A selection of 100X concentrated inhibitor cocktails
• Stabilized and ready-to-use
• No resuspension required

Applications

• Complete phosphatase inhibition in tissue extractions and cell lysis experiments
• Protection of protein phosphatase groups
• Compatible with most common protein assays
• Compatible with IEF/2D studies (PhosphataseArrest™ III)