Human Normal and Tumor Tissue Blots

The premade blots are prepared with the appropriate GenLysate and 50μg protein is loaded onto a 4-20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved they are transferred to a PVDF membrane, using Efficient Western Transfer Buffer.

The blots are ready to be blocked and probed with the antibodies of choice.

Note: We recommend that customers contact our technical department for the latest information on the blots as G-Biosciences reserves the right to change the blot profile due to the availability of GenLysate.

Features

Blot lane order:

1. Protein marker
2. Normal Uterus Lysate
3. Tumor Uterus Lysate
4. Normal Breat Lysate
5. Tumor Breat Lysate
6. Normal Cervix Lysate
7. Tumor Cervix Lysate
8. Normal Ovary Lysate
9. Tumor Ovary Lysate
10. Normal Testis Lysate
11. Tumor Testis Lysate
12. Normal Prostate Lysate
13. Tumor Prostate Lysate
14. Normal Rectum Lysate
15. Tumor Rectum Lysate