Superior™ Blocking Buffer (7 Citations)

Superior™ Blocking Buffer contains a proprietary antiginically non-determinant protein for blocking non-specific sites during ELISA, membrane blotting, immunohistochemistry and other applications. 

Superior™ Blocking Buffer is ideal for a high signal to background ratio in most system. Superior™ Blocking Buffer uses a non-serum protein and does not contain biotin or other animal source proteins to interfere with immuno-complexes. Superior™ Blocking Buffer is suitable for assays that use avidin/streptavidin systems.

Superior™ Blocking Buffer for Precipitating Substrate is a modification of Superior™ Blocking Buffer. This blotting buffer has been optimized for used in blotting protocols that use precipitating substrates, such as TMB (3, 3', 5, 5'-Tetramethylbenzidine) and BCIP (5-Bromo-4-Chloro-3'-Indolyphosphate p-Toluidine Salt) and NBT (Nitro-Blue Tetrazolium Chloride) substrates. Superior™ Blocking Buffer for Precipitating Substrate is not suitable for ELISA or immunohistochemistry staining.

Available in multiple formats using TBS, PBS, TBS with 0.05% Tween^®-20 or PBS with .05% Tween^®-20.  Also supplied as a convenient dry form that is stable at room temperature. Each dry format pack is makes 200ml Superior™ Blocking Buffer.

Features

• Non serum protein blocking agent
• Rapid blocking times; ~2 minutes for ELISA
• Multiple formats; ready-to-use liquid (500ml) or dry buffer packs (for 5 x 200ml)
• Available with and without detergent (Tween^®-20)

Applications

• For blocking Western blot membranes (PVDF and nitrocellulose)
• For blocking and storage of ELISA plates
• For blocking prior to immunohistochemistry staining

Superior™ Blocking Buffer for Precipitating Substrate is not suitable for ELISA or immunohistochemistry staining.