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T4 UvsX DNA Recombinase

T4 UvsX DNA recombinase mediates DNA strand exchange between homologous chromosomes. The protein forms a right-handed nucleoprotein complex on single ssDNA called the presynaptic filament that can search for homology in duplex DNA and pair the recombining DNA molecules to form a DNA joint molecule. This process is aided by ssDNA binding proteins and recombination mediators. The filament is then elongated by DNA polymerase with the newly synthesized strand displacing the old strand. The newly synthesized duplex DNA can then act as a template for the next cycle to achieve exponential amplification of dsDNA

Product Source

E. coli cells expressing recombinant T4 UvsX gene.

Product Includes

  • T4 UvsX DNA Recombinase
  • 10X UvsX DNA Recombinase reaction buffer

10X UvsX DNA Recombinase reaction buffer composition

200 mM Tris-acetate pH 7.8

1M Potassium acetate

100 mM Magnesium acetate

10 mM DTT

Storage Buffer

50 mM Tris-HCl

50 mM KCl

1 mM DTT

0.1 mM EDTA

50% Glycerol

pH 7.5 @ 25ºC

Storage Temperature

- 20ºC

Quality Control assays

UvsX DNA recombinase is free from detectable nuclease contamination.

Material Safety Data Sheet
786-1989
English_US
786-1990
English_US
786-1991
English_US

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