picoLUCENT™ PLUS-AP kit is based on our ultra sensitive Luminol substrate that produces chemiluminescence upon reaction with horseradish peroxidase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.
picoLUCENT™ PLUS-AP is also supplied in a kit format, containing our non-animal protein blocking agent (NAP-BLOCKER™) and wash buffer (femto-TBST™). The picoLUCENT™ PLUS-AP kits allow detection of low picogram levels (10-12) of antigens with low noise (signal/background) ratio. The kit reagents are sufficient for 25 mini blots or 1,500cm2 of PVDF or nitrocellulose membrane. The trial size is suitable for 5 mini blots or 300cm2
For a complete chemiluminescence immunodetection system, we offer optional alkaline phosphatase (AP) conjugated secondary antibodies against mouse, rabbit, human, rat and goat.
Features
- Low picogram detection (10-12)
- Low Background : High signal ratio
- 1 year shelf life
- Reagent for 200cm2 and ≥1,000cm2 membrane
- Nitrocellulose or PVDF compatible
- Supplied with optional HRP or AP conjugated secondary antibodies.
