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OmniPrep™ for Blood

Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community. 

 

A selection of genomic isolation kits are offered that purify high quality genomic DNA  from a wide variety of sources and for a wide array of applications.

 
Our OmniPrep™ genomic DNA kits are for ultra pure genomic DNA that is suitable for all downstream applications.  These blood DNA extraction kits are fully scalable for large genomic DNA isolations. The procedure is slightly more involved to ensure ultra pure DNA.
 
Based on our popular OmniPrep™ system, the OmniPrep™ for Blood kit isolates high quality genomic DNA from blood samples, including whole blood, buffy coats, packed cells and bone marrow. OmniPrep™ for Blood isolates high purity (A260/A280 ratios of 1.8 to 2.0) DNA (about 100kbp) and the yield is between 5 and 30µg/mL, dependent on starting material and quantity. The Omniprep DNA kit is suitable for processing up to 100mL blood.
 
The extraction kit uses a rapid precipitation technique that uses unique precipitation reagents to isolate genomic DNA free from proteins and RNA. Pure genomic DNA is isolated in 20 to 40 minutes, depending on the sample type used. 
 
OmniPrep™ for Blood isolates genomic DNA from:
Whole blood
Buffy coats
Packed cells
Bone marrow

 


Features

  • High Yield: ~100kb genomic DNA 
  • Simple two tube method: 20-40 minutes
  • No toxic chemicals, no phenol, no hazardous waste

 


Applications

  • Extraction of pure genomic DNA from blood 
  • Suitable for whole blood, buffy coats, packed cells and bone marrow
  • Extraction of high quality, 100kb genomic DNA

 

 

Protocol
786-396
Material Safety Data Sheet
786-396
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Technical Literature
Molecular Biology Handbook  A guide to our products for DNA and RNA.

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