Site Catalog Number   Welcome Guest | Register | Login Items:0 View Cart CheckOut My Account   dotMETRICTM A 1µl Protein Assay For Rapid Estimation of Protein Concentration Uses a unique combination of proprietary reagents and test strips. No expensive equipment needed. The method involves mixing of 1µl of protein sample with a specific Dilution Buffer supplied with each kit. Apply 1µl of the solution to the test strip by point-of-contact capillary action. Under a specific buffer condition as protein enters into the matrix of the test strip it binds and saturates as protein diffuses in circular manner. Develop the test strip in 2-8 minutes. A circular protein spot is produced. The diameter of the spot is proportional to the concentration. By measuring the diameter of the spots with the dotMETRICTM scale, supplied with each kit, you can easily determine concentration of protein. EASY TO USE SPOT APPLICATION DEVICE For increased reproducibility and test reliability the dotMETRICTM kits are supplied with an optional Spot Application Device. The Spot Application Device allows application of samples using fixed volume capillary tips and simplifies the task of applying the protein solution on the test strips by point-of-contact capillary action. The Spot Application Device simplifies the application of one or more samples as well as improves reliability of results.  NO PROTEIN-TO-PROTEIN VARIATION Gelatin, BSA, Avidin, Alcohol dehydrogenase (yeast) and Thyroglobulin have been used to measure the diameters of protein spots on the test strip at predetermined concentrations. It has been found that the diameters of protein spots on the test strip are not dependent on the nature and the origin of protein. Since the spot formation is not dependent on the amino acid composition of protein, this property makes the dotMETRICTM assay independent of protein-to-protein variation.   THE dotMETRICTM ASSAY IS RESISTANT TO MOST COMMON LABORATORY AGENTS The dotMETRICTM assay is able to resist common laboratory agents such as Triton-X100, Triton-X114, Thesit, Tween-20, NP-40 and SDS, reducing agents such as beta-mercaptoethanol and DTT, sugars, cobalt, EDTA, Tris buffers, and others.   DLDTM PROTOCOL When protein concentration falls below a level where the diameters of protein spots are no longer large enough to be measured with the dotMETRICTM scale, the assay kit uses an entirely new strategy for determination of protein concentration, called Dilution to the Limit of Detection, or the DLDTM Protocol.  According to the DLDTM Protocol, when a protein solution is serially diluted and spotted onto the test strip, eventually a dilution is reached beyond which the protein spots are not visible; i.e., the dilution has reached the limit of detection (DLDTM). The dilution factor required to reach the limit of detection is used for the determination of protein concentration. FEATURES Sample Economy: Use as little as 1µl of sample. Rapid Assay: Takes 8-10 minutes and can assay as little as 2ng BSA. No Protein-to-Protein Variation: dotMETRICTM assay is independent of protein-to-protein variation. Resistant to Detergents, Reducing Agents & Other Laboratory Agents: Unlike other protein assays, the dotMETRICTM is resistant to detergents and most other common laboratory agents, Triton-X 100,Tween-20,NP-40,SDS, reducing agents, sugars, cobalt and others. Versatile Protein Assay: Because of no protein-to-protein variation and tolerance to a wide variety of common laboratory agents, dotMETRICTM is a versatile protein assay. The dotMETRICTM assay can be performed even if the protein sample is in a SDS-PAGE gel loading buffer.   APPLICATIONS Suitable for rapid estimation of protein concentration. Suitable for determination of protein concentration in cellular fractions, tissue & cell lysates and chromatography purification fractions. Suitable for protein samples containing common laboratory agents, such as: reducing agents, chelating agent -EDTA, detergents, chaotropic agents, and numerous other agents and extraction buffers. Use dotMETRICTM to determine protein concentration in gel loading (SDS-PAGE) sample buffers. Suitable when limited amount of sample is available for analysis, requires only 1µl protein sample. Offers a unique DLD protocol. ACCESSORIES Spot Application Device (Cat.# 786-63)- For use with dotMETRIC Protein assay. Simplifies the application of one or more samples and improves reliability of results. Application Glass Capillary Tips (Cat.# 786-23) 1µl Application glass capillary tips for dotMETRIC Protein assay, for use with Spot Application device. Simplifies the application of one or more samples as well as improves reliability of results. Sample Application (pipet) Tips (Cat.# 786-64) For use with dotMETRIC Protein assay , 1-10µl pipetor tips to be used with standard laboratory pipettes. Developing Trays (Cat.# 786-24) a tray for developing test strips for dotMETRIC protein assay. Catalog # Description Size Shipping Condition Price   786-20 Protein dotMETRIC™ Basic Kit >300 assays Ambient US $179.00 786-21 Basic Kit, with dotMETRIC Spot Application Device >300 assays Ambient US $295.00 786-23 1ul Application Glass Capillary Tips 100 Ambient US $21.00 786-24 Developing Trays 2 Ambient US $12.00 786-63 dotMETRIC Spot Application Device 1 Ambient US $112.00 786-64 Sample Application (pipet) Tips 96 tips Ambient US $15.00 Books | Specials | Order | Register| Contact | MSDS | Employment | Branches and Distributors | Site Map © Geno Technology, Inc. All rights reserved.