Antibody Production & Purification

The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. The most common carrier proteins are bovine serum albumin (BSA) and keyhole limpet hemocyanin (KLH).

 

We offer a wide variety of carrier proteins for the successful generation of antibodies.Our ActiveHOOKproducts include both ActiveHOOK BSA (maleimide activated bovine serum albumin) and ActiveHOOKKLH (maleimide activated keyhole limpet hemocyanin). Both rapidly couple to free sulfhydryl groups on peptides and proteins using just one simple step. These activated carrier proteins save both time and money as separate cross-linkers are not required. Our HyperCarrier uses cationized BSA which has been found to elicit greater immunogenic response compared to normal BSA.

 

HOOK™  kits are also available for the coupling of peptides to carrier proteins. Both amine reactive and sulfhydryl reactive HOOKPeptide Coupling Kits are offered.

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ActiveHOOK™ BSA

ActiveHOOK™ BSA is maleimide activated BSA, by the addition of sulfoSMCC cross-linker. The ActiveHOOK™ BSA rapidly couples to free sulfhydryl groups on peptides and proteins. This act..

ActiveHOOK™ HyperCarrier™

ActiveHOOK™ HyperCarrier™ is maleimide activated HyperCarrier™, by the addition of sulfoSMCC cross-linker. The ActiveHOOK™ HyperCarrier™ rapidly couples to free..

Carbohydrate Coupling Resin

The Carbohydrate Coupling Resin is designed for the simple and efficient coupling of glycoproteins to a solid agarose support through oxidized sugar groups.  the coupling is through the hydrazide..

F(ab)2 Fragmentation

The F(ab)2 Fragmentation kits are designed for the generation and isolation of F(ab)2 fragments from IgG molecules.   The kit utilizes our Immobilized Pepsin resin.  Pepsin is a proteolyti..

Fab & F(ab)2 Fragmentation of Mouse IgG1

The Fab & F(ab)2 Fragmentation of Mouse IgG1 kit is designed for the generation and isolation of Fab and F(ab)2 fragments from mouse IgG1 molecules.    The kit utilizes our Immobilized..

Fab Fragmentation

The Fab Fragmentation kits are designed for the generation and isolation of Fab fragments from IgG molecules.    The kits utilize our Immobilized Papain resin.  Papain is a cysteine p..

HOOK™ IgG Biotinyaltion (Sulfhydryl)

Simple Antibody Biotinylation   The HOOK™ IgG Biotinylation (Sulfhydryl) kit is designed for the efficient biotinylation of IgG molecules by immobilizing the IgG molecules on a solid supp..

HOOK™ IgG Biotinylation (Amine)

Simple Antibody Biotinylation   The HOOK™ IgG Biotinylation (Amine) kit is designed for the efficient biotinylation of IgG molecules by immobilizing the IgG molecules on a solid support (..

IgG Binding, Wash & Elution Buffers

G-Biosciences IgG Binding, Wash & Elution Buffers are designed for the non-denaturing, high yield purification of antibodies from IgG affinity purification resins.  The buffers are ideal for ..

Immobilized Ficin

Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge reason.  Ficin is ty..

Immobilized Jacalin

Jacalin, or Artocarpus integrifolia lectin, is a tetrameric two-chain lectin with a molecular weight of 66kDa. Jacalin is a α-D-galactose binding lectin purified from jack-fruit (Artocarpus int..

Immobilized Papain

Papain is a cysteine protease enzyme (EC 3.4.22.2) that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region.  This cleavage results in the generation of three ..