Antibody Production & Purification

The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. The most common carrier proteins are bovine serum albumin (BSA) and keyhole limpet hemocyanin (KLH).

 

We offer a wide variety of carrier proteins for the successful generation of antibodies.Our ActiveHOOKproducts include both ActiveHOOK BSA (maleimide activated bovine serum albumin) and ActiveHOOKKLH (maleimide activated keyhole limpet hemocyanin). Both rapidly couple to free sulfhydryl groups on peptides and proteins using just one simple step. These activated carrier proteins save both time and money as separate cross-linkers are not required. Our HyperCarrier uses cationized BSA which has been found to elicit greater immunogenic response compared to normal BSA.

 

HOOK™  kits are also available for the coupling of peptides to carrier proteins. Both amine reactive and sulfhydryl reactive HOOKPeptide Coupling Kits are offered.

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ActiveHOOK™ BSA

ActiveHOOK™ BSA is maleimide activated BSA, by the addition of sulfoSMCC cross-linker. The ActiveHOOK™ BSA rapidly couples to free sulfhydryl groups on peptides and proteins. This act..

ActiveHOOK™ HyperCarrier™

ActiveHOOK™ HyperCarrier™ is maleimide activated HyperCarrier™, by the addition of sulfoSMCC cross-linker. The ActiveHOOK™ HyperCarrier™ rapidly couples to free..

ActiveHOOK™ KLH

ActiveHOOK™ KLH is maleimide activated by the addition of sulfoSMCC cross-linker. The ActiveHOOK™ KLH rapidly couples to free sulfhydryl groups on peptides and proteins. This act..

Alum Adjuvant

Alum Adjuvant is insoluble white colloidal suspension of aluminum hydroxide used to potentiate immune response. Antigen adsorbs on the aluminum hydroxide precipitate and when injected form a depot of ..

Bovine Serum Albumin (BSA)

Bovine serum albumin is a single polypeptide of 67kDa and consists of 59 lysine residues, of which 30-35 have primary amines that can react with crosslinkers for the coupling of peptides.   ..

Carbohydrate Coupling Resin

The Carbohydrate Coupling Resin is designed for the simple and efficient coupling of glycoproteins to a solid agarose support through oxidized sugar groups. The coupling is through the hydrazide group..

Carboxyl Coupling Resin (Immobilized DADPA (Diaminodipropylamine))

The Carboxyl Coupling Resin consists of 6% cross-linked agarose with covalent linked diaminodipropylamine (DADPA) to generate a free primary amine at the end of a long spacer arm. This resin is also t..

CNBr-Activated Agarose (Dry Form)

CNBr-Activated Agarose is pre-activated high capacity resin used for covalent coupling of antibodies and large protein molecules for affinity chromatography.    Cyanogen bromide r..

Epoxy-Activated Agarose (Dry Form)

Epoxy-Activated Agarose is preactivated resin used for affinity chromatography. Epoxy-Activated Agarose comprise of high density epoxy groups that form covalent bonds with ligands containing amine gro..

F(ab)2 Fragmentation

The F(ab)2 Fragmentation kits are designed for the generation and isolation of F(ab)2 fragments from IgG molecules.   The kit utilizes our Immobilized Pepsin resin.  Pepsin is a proteo..

Fab and F(ab)2 Fragmentation of Mouse IgG1

The Fab & F(ab)2 Fragmentation of Mouse IgG1 kit is designed for the generation and isolation of Fab and F(ab)2 fragments from mouse IgG1 molecules.  The kit utilizes our Immobilized Ficin..

Fab Fragmentation

The Fab Fragmentation kits are designed for the generation and isolation of Fab fragments from IgG molecules.    The kits utilize our Immobilized Papain resin.  Papain is a cystei..