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Antibody Production & Purification

The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. The most common carrier proteins are bovine serum albumin (BSA) and keyhole limpet hemocyanin (KLH).

We offer a wide variety of carrier proteins for the successful generation of antibodies.Our ActiveHOOKproducts include both ActiveHOOK BSA (maleimide activated bovine serum albumin) and ActiveHOOKKLH (maleimide activated keyhole limpet hemocyanin). Both rapidly couple to free sulfhydryl groups on peptides and proteins using just one simple step. These activated carrier proteins save both time and money as separate cross-linkers are not required. Our HyperCarrier uses cationized BSA which has been found to elicit greater immunogenic response compared to normal BSA.

HOOK™  kits are also available for the coupling of peptides to carrier proteins. Both amine reactive and sulfhydryl reactive HOOKPeptide Coupling Kits are offered.

 

Also available are Immobilized Protein A/GImmobilized Protein A and Immobilized Protein G, each crosslinked to agarose beads.  Or, Protein A Magnetic Beads, Protein G Magnetic Beads, and Protein A/G Magnetic Beads for rapid and affordable isolation of antibodies.

 

Download the Antibody Production Handbook for Complete Product Listing

ActiveHOOK™ BSA is maleimide activated BSA, by the addition of sulfoSMCC cross-linker. The ActiveHOOK™ BSA rapidly couples to free sulfhydryl groups on peptides and proteins. This activated carrier proteins save both time and money as separate cross-linkers are not required and the ..
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ActiveHOOK™ HyperCarrier™ is maleimide activated HyperCarrier™, by the addition of sulfoSMCC cross-linker. The ActiveHOOK™ HyperCarrier™ rapidly couples to free sulfhydryl groups on peptides and proteins. This activated carrier proteins save both time and mone..
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ActiveHOOK™ KLH is maleimide activated by the addition of sulfoSMCC cross-linker. The ActiveHOOK™ KLH rapidly couples to free sulfhydryl groups on peptides and proteins. This activated carrier proteins save both time and money as separate cross-linkers are not required and the ..
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Gene/Protein:
Alum Adjuvant is insoluble white colloidal suspension of aluminum hydroxide used to potentiate immune response. Antigen adsorbs on the aluminum hydroxide precipitate and when injected form a depot of insoluble antigen at injection site which attracts immune cells and enhance immune response. It is u..
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Gene/Protein:
Bovine serum albumin is a single polypeptide of 67kDa and consists of 59 lysine residues, of which 30-35 have primary amines that can react with crosslinkers for the coupling of peptides.   OneQuant™ BSA The BSA carrier protein is also available as our single use OneQuant™ ..
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The Carboxyl Coupling Resin consists of 6% cross-linked agarose with covalent linked diaminodipropylamine (DADPA) to generate a free primary amine at the end of a long spacer arm. This resin is also termed as EAH-Agarose.   Molecules, including proteins, peptides and nucleic acids, are c..
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CNBr-Activated Agarose is pre-activated high capacity resin used for covalent coupling of antibodies and large protein molecules for affinity chromatography.    Cyanogen bromide reacts with hydroxyl groups of resin to form cyanate esters which inturn reacts with primary amine ligand..
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Epoxy-Activated Agarose is preactivated resin used for affinity chromatography. Epoxy-Activated Agarose comprise of high density epoxy groups that form covalent bonds with ligands containing amine groups, thiol or hydroxyl groups.   Epoxide-Acivated Agarose is prepared by immobilization of ox..
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The F(ab)2 Fragmentation kits are designed for the generation and isolation of F(ab)2 fragments from IgG molecules.   The kit utilizes our Immobilized Pepsin resin.  Pepsin is a proteolytic enzyme that is routinely used for the generation of F(ab)2 fragments from immunoglobulin G (IgG).&..
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Gene/Protein:
The Fab & F(ab)2 Fragmentation of Mouse IgG1 kit is designed for the generation and isolation of Fab and F(ab)2 fragments from mouse IgG1 molecules.  The kit utilizes our Immobilized Ficin resin.  Ficin (or Ficain) (~25,000Da) is a cysteine protease enzyme (EC 3.4.22.3) isolated fro..
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The Fab Fragmentation kits are designed for the generation and isolation of Fab fragments from IgG molecules.    The kits utilize our Immobilized Papain resin.  Papain is a cysteine protease enzyme (EC 3.4.22.2) that has the endopeptidase activity to cleave immunoglobulin G molecule..
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G-Alum™ Adjuvant Kit is alum-antigen precipitate kit where in alum-antigens complex is co-precipitated as gel. The kit has two components Aluminum Solution and Precipitating Agent. G-Alum™ Adjuvant Kit is used to raise polyclonal and monoclonal antibodies.   Alum-antigen precipita..
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