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Bioassays

Download the Bioassay Handbook & Selection Guide for Complete Product Listing

 

Our Bioassays cover a wide range of proteomic and cell biology fields. Our CytoScan products offer a range of
non-radioactive bioassays for the study of chemical and cell-mediated cytotoxicity, viability, cell death or cell proliferation. Included are cell proliferation and cell toxicity assays which feature lactate dehydrogenase (LDH) assays, WST-1 assays and  sulforhodamine B (SRB) assays. Our apoptotic line features caspase assays and a wide selection of inhibitors.

 
An array of highly sensitive protein quantification assays are available including NI(Non-Interfering) protein assay, a colorimetric protein assay that overcomes interference of common laboratory agents, and our CB-X  assay which is designed to be compatible with all commonly used buffers and conditions.

 

We offer continuous enzyme coupled assays for methylation that can continuously monitor SAM-dependent methyltransferases without the use of radioactive labels or endpoint measurements.

 

We have a phosphatase assay for the quantification of phosphatases and three phosphatase inhibitor cocktails to inhibit phosphatases and for phosphatase studies.

 
PEROXsay Peroxide Assays and a variety of enzymes are also available.
A class of polypeptide antibiotics isolated from the Steptomyces soil bacteria.  Actinomycin D inhibits transcription by binding to DNA at the transcription initiation complex and therefore preventing elongation by the RNA polymerase.   Supplied as 50μl 10mM solution.   Featur..
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Adenine Deaminase (EC 3.5.4.2) converts Adenine to Hypoxanthine.   A highly purified recombinant enzyme protein supplied at a 2µM concentration...
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Adenosylhomocysteine nucleosidase (EC 3.2.2.9) converts S-adenosylhomocysteine (AdoHcy) to S-(deoxy-D-ribos-5-yl)-L-homocysteine + adenine.   A highly purified recombinant enzyme protein supplied at a 10µM concentration...
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AlamarBlue Cell Viability Assay reagent quantitatively measures the proliferation of mammalian cell lines, bacteria and fungi. The dye in the Alamar blue assay incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and changes color in response to the chemical reduction of growth..
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The Apoptotic DNA Ladder kit contains all of the reagents necessary for the preparation of cellular fractions for the production of apoptosis nucleosomal DNA ladders. The kit does not require the use of toxic phenol. The protocol involves: cell lysis; removal of cellular debris; and precipitation of..
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Ready-to-use high quality apoptosis inducers allow users an easy tool for induction of apoptosis in cultured cells. These stabilized reagent solutions have a long storage life and are easy to use. The selection of apoptosis inducers include actinomycin D, camptothecin, cycloheximide, dexamethasone, ..
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4-ethyl-4-hydroxy-1H-pyrano[3’,4’:6,7]indolizino[1,2-b] quinoline-3,14-(4H,12H)-dione     A cytotoxic quinoline alkaloid that inhibits the DNA enzyme topoisomerase I (Topo I). Supplied as 1ml 2mM   Features Synonym: CPT CAS Number: 7689-03-4 Linear Formula: C20H1..
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Catalase is an enzyme present in blood and other tissues with antioxidant activity. This enzyme can catalyze the reaction that consumes hydrogen peroxide, transforming it into water and oxygen. Since hydrogen peroxide is difficult to be measured directly in biological samples, the determination of t..
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An improved Coomassie Dye based protein assay based on the Bradford Protein Assay (1). This assay is suitable for the simple and rapid estimation of protein concentration. This assay is based on a single Coomassie dye based reagent. The binding of protein to the dye results in a change of color from..
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Free radicals are highly reactive species that are naturally formed during cell metabolism in the mitochondria, secretion by immune cells during inflammatory processes or taken up as environmental pollutants. CUPRAC (CUPric Reducing Antioxidant Capacity) Assay is a new method, which enables the To..
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(4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione)     An inhibitor of protein synthesis by interfering with the translocation step (movement of two tRNA molecules and mRNA on ribosome) in protein synthesis, thus blocking translational elongation. &..
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CytoScan™-SRB Cytotoxicity Assay is an accurate and reproducible assay based upon the quantitative staining of cellular proteins by sulforhodamine B (SRB).   This assay has been used for high-throughput drug screening at the National Cancer Institute (NCI) (1). Sulforhodamine B is an ..
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