2D Electrophoresis

G-Biosciences has developed a wide range of products that are specifically designed for isoelectric focusing (IEF) and 2D electrophoresis. All these products have the brand name FOCUS and are manufactured under strict quality control conditions and thoroughly tested to ensure the highest 2D gel electrophoresis performance.

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2D-Detergent™ Nonidet® P-40 Substitute

Many commercial grade detergents contain elevated levels of sulfhydryl oxidizing agents, peroxides, salts and carbonyl compounds (Figure 1). The proteins that are isolated with these detergents are hi..

2D-Detergent™ Triton® X-100

Many commercial grade detergents contain elevated levels of sulfhydryl oxidizing agents, peroxides, salts and carbonyl compounds (Figure 1). The proteins that are isolated with these detergents are hi..

2D-Xtract™

A suitable protein solubilization buffer for 2D analysis must solubilize proteins effectively, without disturbing the native charge of the proteins.  Urea based solubilization buffers solubilize ..

Acrylamide

Features CAS #: 79-06-1 Chemical Formula: C3H5NO Molecular Weight: 71.08   ..

Agarose Sealing Solution

Simply heat and use for sealing the IPG strips for SDS-PAGE analysis. The Agarose Sealing Solution is prepared in a proprietary buffer to minimize reoxidation of the competing thiol pairs as proteins ..

Equilibration Buffer for IPG Strips

Add an appropriate volume of the supplied rehydration buffer to the dry, ready-to-use, urea based equilibration buffer.   Eliminates worries about urea induced protein carbamylation or waste of..

FOCUS™ Protein Alkylation

The kit is supplied with a proprietary buffer necessary for efficient alkylation of thiols, while minimizing reoxidation of the competing thiol pairs in protein samples. Simply add an appropriate amou..

FOCUS™ Protein Reductant

A water soluble, odorless, non-toxic and stable TCEP [Tris (2-carboxyethyl) phosphine] reductant for protein reduction.   FOCUS™ Protein Reductant has improved stability and efficiency compare..

FOCUS™ Protein Reduction-Alkylation Kit

The FOCUS™ Reduction-Alkylation kit offers a simple two-step method for reduction and alkylation of protein samples for 2D gel analysis.   The disulfide bonds are reduced with a highly re..

Immobilized Reductant

Reducing agents are used in the reduction of disulfide bonds of proteins and peptides.  It is often necessary to remove the reducing agents from the protein/peptide solutions to prevent them from..

InGel™ Blue

The InGel™ Blue kit provides a complete set of reagents for the in gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS).  The kit is specifically desi..

Perfect-FOCUS™

Protein samples loaded on iso-electric focusing (IEF) gels should ideally have low conductivity and be free from agents known to interfere with net protein charge. Protein samples containing interferi..