G-Biosciences offer a large selection of 2D gel electrophoresis (2DGE) and IEF optimized reagents for the crucial preparation of native protein samples. We provide 2DGE sample clean up to ensure high quality spot resolution. We offer mass spectrometry compatible protein stains for spot identification and proprietary InGel digestion kits for both Coomassie and Silver stained gels.
Proper preparation of protein samples for 2DGE is integral to the success of your experiment. We offer IEF and 2DGE optimized detergents such as 2-D Detergent™ Nonidet® P-40 Substitute and 2-D Detergent™ Triton® X-100 which contain reduced peroxides, carbonyl compounds and have less than 15µS conductivity.
The IEF and 2DGE clean up kit, Perfect-FOCUS™, is optimized for the preparation of low conductivity protein samples which are free from detergents, salts, peptides, nucleic acids, lipids, phenols, and other small charged molecules.
Our 2DGE and mass spectrometry compatible FOCUS™ FASTsilver™ produces crystal clear backgrounds without inhibiting downstream proteolytic digestion required for mass spectrometry.
A variety of additional 2DGE reagents such as FOCUS™ Extraction buffers, 2D-Xtract™, FOCUS™ Protein Reductant, FOCUS™ Protein Alkylation, FOCUS™ DTT, Proteomic grade water are specifically optimized to retain the native charge of your protein of interest. Convenient kits for reduction and alkylation of samples for enhanced protein spot resolution are also available.
G-Biosciences manufacture the 2DGE and IEF optimized FOCUS™ brand reagents under strict quality control conditions to ensure the highest 2DGE performance.