2D Electrophoresis

G-Biosciences has developed a wide range of products that are specifically designed for isoelectric focusing (IEF) and 2D electrophoresis. All these products have the brand name FOCUS and are manufactured under strict quality control conditions and thoroughly tested to ensure the highest 2D gel electrophoresis performance.

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Acrylamide

Features CAS #: 79-06-1 Chemical Formula: C3H5NO Molecular Weight: 71.08   ..

Agarose Sealing Solution

Simply heat and use for sealing the IPG strips for SDS-PAGE analysis. The Agarose Sealing Solution is prepared in a proprietary buffer to minimize reoxidation of the competing thiol pairs as proteins ..

Equilibration Buffer for IPG Strips

Add an appropriate volume of the supplied rehydration buffer to the dry, ready-to-use, urea based equilibration buffer.   Eliminates worries about urea induced protein carbamylation or waste of..

FOCUS™ Protein Alkylation

The kit is supplied with a proprietary buffer necessary for efficient alkylation of thiols, while minimizing reoxidation of the competing thiol pairs in protein samples. Simply add an appropriate amou..

FOCUS™ Protein Reduction-Alkylation Kit

The FOCUS™ Reduction-Alkylation kit offers a simple two-step method for reduction and alkylation of protein samples for 2D gel analysis.   The disulfide bonds are reduced with a highly re..

Proteomic Grade Microfuge Tubes

1.5ml centrifuge tubes that are certified protein/dust free. Suitable for proteomic analysis and research. 100 tubes are supplied...

Water, Proteomic Grade

Proteomic Grade Water suitable for 2D electrophoresis and mass spectrometry analysis. Removes worries of protien and dust contamination and improves quality and reproducibility of 2D..

2D-Detergent™ Nonidet® P-40 Substitute

Many commercial grade detergents contain elevated levels of sulfhydryl oxidizing agents, peroxides, salts and carbonyl compounds (Figure 1). The proteins that are isolated with these detergents are hi..

2D-Detergent™ Triton® X-100

Many commercial grade detergents contain elevated levels of sulfhydryl oxidizing agents, peroxides, salts and carbonyl compounds (Figure 1). The proteins that are isolated with these detergents are hi..

2D-Xtract™

A suitable protein solubilization buffer for 2D analysis must solubilize proteins effectively, without disturbing the native charge of the proteins. Urea based solubilization buffers solubilize protei..

FOCUS™ DTT [2M]

For those who prefer DTT as a reducing agent, we offer ultra pure proteomic grade DTT for protein reduction. FOCUS™ DTT is supplied as 5 vials containing dry powder to maintain the activity of t..

FOCUS™ Extraction Buffers

Chaotropic extraction buffers that preserve the native charge of proteins making them suitable for sample extraction for 2D gels   One of the most important considerations before running 2D..