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Plasmid Purification & Colony Screening

Download the Molecular Biology Handbook for Complete Product Listing

 

Plasmid Isolation

Spin column format miniprep plasmid isolation kits from bacteria available are GET™ Plasmid DNA Miniprep for up to 20µg DNA from 1-5ml culture and a 96-well plate format, GET™ Plasmid DNA 96-Well. Select the EZ Yeast™ Plasmid Prep kit for yeast plasmid purification.

Alternatively, our Silica Magentic Beads can be used for the purification of plasmid DNA following alkaline lysis.

Plasmid Transformation & Transfection

 Z-Competent™ E. coli Transformation kit is designed to generate competent E. coli cells for simple and highly efficient E. coli transformation.

G-FECT™ DNA Transfection reagents are a series of powerful DNA transfection reagents for the effective and reproducible transfection of DNA into mammalian cells.  G-FECT™ can be used for plasmid transfection, genome editing, virus production and more. 

Colony Screening

A large selection of antibiotics are offered for colony selection, including HP Ampicillin™, a stronger and more stable inhibitor of ß-lactamase than regular ampicillin.

For blue/white colony screening, a molecular biology grade IPTG is available. 

For rapid colony screening, including PCR-colony screening, we recommend our Plasmid Screening ToothPick™ or ToothPick™-PCR for the rapid analysis of single colonies by restriction digestion or PCR. No need for an overnight culture.

Gene/Protein:
The EZ Yeast™ Plasmid Prep kit is designed for the rapid isolation of 2μ plasmids from yeast patches or yeast grown in small liquid cultures. The kit is adapted from the alkaline lysis of E. coli, by providing modified alkaline lysis buffers and our LongLife™ Zymolyase®, a highly ..
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Gene/Protein:
GET™ Plasmid DNA 96-Well kit is designed for the high throughput preparation of plasmid DNA from 96 x 1-5ml E. coli cultures. The kit utilizes a 96 well format enhanced DNA binding plate to produce high yields of plasmid. This quick and easy protocol eliminates toxic phenol/chloroform ext..
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Isolates high quality plasmid DNA from 1-5ml E. coli cultures. The kit utilizes an enhanced DNA binding column to produce high yields of plasmid. This quick and easy protocol eliminates toxic phenol/ chloroform extractions or lengthy ethanol precipitations. On completion of the protocol, the plasmid..
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HP Ampicillin™ is a stronger and more stable inhibitor of ß-lactamase than regular ampicillin and, therefore, supports only the growth of ampicillin resistant colonies even after prolonged incubation.   Culture plates can be incubated for a longer period at 37°C, eliminating t..
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Gene/Protein:
Plasmid Screening Toothpick™ is a unique system that allows for the rapid screening of bacteria for transformed plasmids, without the need for an overnight culture. Pick a colony, add to Plasmid Screening Toothpick™ reagents and then analyze using restriction enzymes. Features ..
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G-Biosciences Silica Magnetic Beads are Fe3O4 magnetic beads coated with a silicon dioxide (SiO2) layer. Since silica is able to bind to the nucleic acids, G-Biosciences Silica Magnetic Beads serve as a simple and efficient tool for plasmid DNA purification for transfection or sequencing application..
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Gene/Protein:
Toothpick™-PCR is an extension of Toothpick™ and allows for the rapid release of plasmids from transformed bacteria for screening by polymerase chain reaction (PCR). There is no requirement for growing bacteria, performing "minipreps" or purifying the plasmid DNA.   ..
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The Z-Competent™ E. coli Transformation kit is designed to generate competent E. coli cells for simple and highly efficient E. coli transformation.   E. coli are grown in SOB medium (supplied with Cat. # GZ-5), washed and suspended in our supplied Competent Buffer. The bacterial cells a..
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Gene/Protein:
IPTG (Isopropyl β-D-1-thiogalactopyranoside) is a useful molecular biology reagent. IPTG is a molecular mimic of allolactose that initiates transcription of the lac operon.  The key feature of IPTG is that, unlike allolactose, is not hydrolyzed by ß-galactosidase, therefore its ..
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