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Protein cross-linking and modification reactions often required carefully controlled reaction conditions as some buffers will interfere with coupling and modification reactions.  For example, Tris buffers will interfere with amine coupling reactions. 
G-Biosciences offers a selection of optimized buffers designed for specific reactions. 
G-Biosciences also offers a reactor system, Tube-O-Reactor™, that is ideal for protein cross-linking and modification reactions.
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DMF

Anhydrous DMF [N,N-Dimethylformamide (HCON(CH3)2)]. Suitable for cross-linking, protein modification and biotinylation reaction applications...

Optimizer Buffer™

The conjugation and modification reactions used to cross-link proteins or couple labels to proteins, such as biotin, enzymes, and fluorescent dyes, require certain conditions, including pH and chemica..

TNBS

TNBS (2,4,6-trinitrobenzene sulfonic acid) is a highly sensitive and rapid chemical used to quantitate the free amino groups.  The reaction of TNBS with primary amines generates a highly chromoge..

Tube-O-Reactor™

Tube-O-Reactor™ is a system that allows all the key steps of cross linking, coupling and modification of proteins and/or nucleic acids to be performed in a single tube. This minimizes the risk of samp..

DMSO

Anydrous DMSO [Dimethyl sulfoxide (CH3)2SO] is offered for the solubilization of the water insoluble cross-linkers and modification reagents.     ..

SpinOUT™ Columns

Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.  The SpinOUT™ GT-100, GT-600 and GT-1200 columns..

Tube-O-DIALYZER™

Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.  Dialysis is a popular technique used for the exchange..