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Download the Crosslinkers Handbook & Selection Guide            Download the Protein Labeling & Conjugation Handbook

 

Protein cross-linking and modification reactions often required carefully controlled reaction conditions as some buffers will interfere with coupling and modification reactions.  For example, Tris buffers will interfere with amine coupling reactions. 
G-Biosciences offers a selection of optimized buffers designed for specific reactions. 
G-Biosciences also offers a reactor system, Tube-O-Reactor™, that is ideal for protein cross-linking and modification reactions.

Anhydrous DMF [N,N-Dimethylformamide (HCON(CH3)2)]. Suitable for cross-linking, protein modification and biotinylation reaction applications...
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Anydrous DMSO [Dimethyl sulfoxide (CH3)2SO] is offered for the solubilization of the water insoluble cross-linkers and modification reagents.    ..
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The conjugation and modification reactions used to cross-link proteins or couple labels to proteins, such as biotin, enzymes, and fluorescent dyes, require certain conditions, including pH and chemical composition, for optimal conjugation. Many common buffers routinely used in laboratories have an i..
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Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.    The SpinOUT™ GT-100, GT-600 and GT-1200 columns are versatile, spin-format columns for the desalting and buffer exchange of peptide, protein ..
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TNBS (2,4,6-trinitrobenzene sulfonic acid) is a highly sensitive and rapid chemical used to quantitate the free amino groups.  The reaction of TNBS with primary amines generates a highly chromogenic product that can be readily measured at 335nm. Supplied as a 1% solution in methanol.   ..
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Tube-O-Reactor™ is a system that allows all the key steps of cross linking, coupling and modification of proteins and/or nucleic acids to be performed in a single tube. This minimizes the risk of sample loss, of experimental time and of hands-on phases.   Most of the above reactions inv..
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Model:
The SpinOUT™ G-Acryl columns are versatile, spin-format columns for the desalting and buffer exchange of peptide and protein and other macromolecule solutions ranging from 5µl through to 3ml sample volumes.  The SpinOUT™ columns are available in three MWCO sizes for >1,800,..
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Model:
Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.    Dialysis is a popular technique used for the exchange of buffer medium across semi-permeable membranes. Dialysis devices are available in many ..
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