Affinity Purification Resins

For the separation & purification of affinity tagged proteins & antibodies
 
The separation and purification of proteins has been a major challenge for researchers in the early days of proteomic research. The advances in affinity chromatography have enabled researchers to purify large quantities of highly pure proteins for a multitude of analysis techniques, including crystallography, protein:protein studies and in-vitro assays.
Affinity chromatography works by binding a protein, via a reversible interaction, to a specific ligand that is prebound to a solid chromatographic support. The protein(s) are first bound to the column in a buffer that supplies conditions optimal for binding. Unbound, non-specific material is washed away and the protein(s) of interest are then eluted by changing the buffering conditions to induce desorption from the solid support.
We supply two main groups of affinity resins. The first group is for the separation and purification of affinity tagged proteins and the second group is for the binding of immunoglobulin molecules.
A common practice in today’s research is the use of molecular biology to clone our protein(s) of interest into a vector that adds a specific tag to the protein. The most versatile and common tags used are glutathione S-transferase (GST), a 6x histidine motif (His-tag), and the calmodulin binding peptide (CBP).
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Glutathione, Reduced

Synonym: γ-L-Glutamyl-L-cysteinyl-glycine, GSH   Amino Acid Sequence: γ-Glu-Cys-Gly   CAS Number: 70-18-8   Linear Formula: C10H17N3O6S   Molecular Weight: 307...

GST Elution Buffer

Features Supplied with dry glutathione to limit oxidation Glutathione resin also available ..

Amine Magnetic Beads

G-Biosciences’ Amine Magnetic Beads are 1µm, uniform magnetic beads with an amine (-NH2) surface functional group. The magnetic beads consist of a single-crystal Fe3O4 sphere core and..

Boronate Resin

Our Boronate resin is designed for the isolation of ribonucleotide and oligonucleotide RNA.   The resin consists of boronic acid covalently linked to a polyacrylamide support that offe..

Calmodulin Binding/ Wash Buffer

Features 50mM Tris-HCl (pH7.5), 200mM NaCl, 2mM CaCl2 Also available are Calmodulin Resin Kits     ..

Calmodulin Elution Buffer

Features 50mM Tris-HCl (pH7.5), 200mM NaCl, 2mM EGTA Also available are Calmodulin Resin Kits     ..

Calmodulin Resin

Calmodulin Resin for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells. The resin is 4..

Carbohydrate Coupling Resin

The Carbohydrate Coupling Resin is designed for the simple and efficient coupling of glycoproteins to a solid agarose support through oxidized sugar groups. The coupling is through the hydrazide group..

Carboxyl Coupling Resin (Immobilized DADPA (Diaminodipropylamine))

The Carboxyl Coupling Resin consists of 6% cross-linked agarose with covalent linked diaminodipropylamine (DADPA) to generate a free primary amine at the end of a long spacer arm. This resin is also t..

Carboxyl Immobilization Kit

G-Biosciences Carboxyl Immobilization kit utilizes the Carboxyl Coupling Resin that consists of 6% cross-linked agarose with covalent linked diaminodipropylamine (DADPA) to generate a free primary ami..

Carboxyl Magnetic Beads

G-Biosciences’ Carboxyl Magnetic Beads are 1µm, uniform magnetic beads with surface functional group -COOH. The magnetic bead consists of a single-crystal Fe3O4 sphere core and dextran coa..

CDI Amine Reactive Agarose

G-Biosciences CDI Amine Reactive Agarose consists of 6% cross-linked agarose activated with CDI (1,1’-carbonyl diimidazole) to form reactive imidazole carbamates.    The activati..