menu
Your Cart

Affinity Purification Resins

Download the Protein Purification Handbook for a full list of related productsDownload the Purification Resins Handbook for Complete Product List

 

For the separation & purification of affinity tagged proteins & antibodies
 
The separation and purification of proteins has been a major challenge for researchers in the early days of proteomic research. The advances in affinity chromatography have enabled researchers to purify large quantities of highly pure proteins for a multitude of analysis techniques, including crystallography, protein:protein studies and in-vitro assays.
Affinity chromatography works by binding a protein, via a reversible interaction, to a specific ligand that is prebound to a solid chromatographic support. The protein(s) are first bound to the column in a buffer that supplies conditions optimal for binding. Unbound, non-specific material is washed away and the protein(s) of interest are then eluted by changing the buffering conditions to induce desorption from the solid support.
We supply two main groups of affinity resins. The first group is for the separation and purification of affinity tagged proteins and the second group is for the binding of immunoglobulin molecules.
A common practice in today’s research is the use of molecular biology to clone our protein(s) of interest into a vector that adds a specific tag to the protein. The most versatile and common tags used are glutathione S-transferase (GST), a 6x histidine motif (His-tag), and the calmodulin binding peptide (CBP).
G-Biosciences’ Amine Magnetic Beads are 1µm, uniform magnetic beads with an amine (-NH2) surface functional group. The magnetic beads consist of a single-crystal Fe3O4 sphere core and dextran coating layer. Through chemical modification of dextran, the primary amino group (-NH2) are..
$0.00
Our Boronate resin is designed for the isolation of ribonucleotide and oligonucleotide RNA.   The resin consists of boronic acid covalently linked to a polyacrylamide support that offers simple isolation of small molecular weight compounds that have cis-diol groups.   Mechanism: T..
$0.00
Calmodulin Resin for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells. The resin is 4% agarose coupled to calmodulin and has a ligand density of approximately 1mg calmodulin/ml resin. ..
$0.00
The Carbohydrate Coupling Resin is designed for the simple and efficient coupling of glycoproteins to a solid agarose support through oxidized sugar groups. The coupling is through the hydrazide group that is immobilized to agarose beads. The resin is ideal for immobilized polyclonal antibodies as t..
$0.00
The Carboxyl Coupling Resin consists of 6% cross-linked agarose with covalent linked diaminodipropylamine (DADPA) to generate a free primary amine at the end of a long spacer arm. This resin is also termed as EAH-Agarose.   Molecules, including proteins, peptides and nucleic acids, are c..
$0.00
G-Biosciences Carboxyl Immobilization kit utilizes the Carboxyl Coupling Resin that consists of 6% cross-linked agarose with covalent linked diaminodipropylamine (DADPA) to generate a free primary amine at the end of a long spacer arm. Molecules, including proteins and peptides, are covalently ..
$0.00
G-Biosciences’ Carboxyl Magnetic Beads are 1µm, uniform magnetic beads with surface functional group -COOH. The magnetic bead consists of a single-crystal Fe3O4 sphere core and dextran coating layer. Through chemical modification of dextran, the carboxyl groups (-COOH) are joined to..
$0.00
G-Biosciences CDI Amine Reactive Agarose consists of 6% cross-linked agarose activated with CDI (1,1’-carbonyl diimidazole) to form reactive imidazole carbamates.    The activation of the resin occurs in solvent and to maintain its activity the resin is supplied in acetone to preve..
$0.00
Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.     The Co-NTA Resin is specifically designed for the purif..
$0.00
Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.    The Cobalt Chelating Resin is specifically designed for the..
$0.00
Concanavalin A (Con A) Agarose consists of Con A coupled to 4% agarose by the cyanogen bromide method.  Con A is a tetrameric metalloprotein lectin isolated from Canavalia ensiformis (jack bean).   Con A is used for the purification of glycoproteins, polysaccharides and glycolipids as it..
$0.00
Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath et al (1), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.  The Copper Chelating Resin is specifically designed for the pu..
$0.00
Showing 1 to 12 of 57 (5 Pages)

Latest Blog

Benchtop Lab Equipment

Teaching Biotechnology

Connect With Us

Stay up to date with G-Biosciences by signing up for our newsletter.