Molecular and Genomic Studies

Our Bioscience Excellence Molecular and Genomic Studies Kits introduce students to molecular cloning techniques, including restriction digestion, ligation, bacterial transformation and protein expression.  Other techniques include the polymerase chain reaction and bacterial conjugation.  

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Bacterial Conjugation

Bacterial conjugation is a naturally occurring process that allows the transfer of DNA from one bacterium to another, which allows the transfer of genetic traits, particularly drug resistance.  ..

Onion or Bacterial Genomic DNA Isolation

Isolation of genomic DNA is an essential technique in modern research science, particularly molecular biology and biotechnology.    Genomic DNA is purified from a multitude of sources incl..

Purification of a Gene

Agarose electrophoresis is an important tool for isolating DNA by its molecular weight; following separation of DNA molecules, the DNA needs to be purified from the agarose for further applications. ..

Bacterial Transformation

Bacterial transformation allows researchers to insert their recombinant DNA into bacteria, which then multiply making more copies of the transformed bacteria. The transformed plasmid can also be used ..

Confirming the Amylase gene using PCR (Lab 13h)

This kit is designed to teach 8 groups of four students the principles of the polymerase chain reaction (PCR)   The kit contains the reagents to allow students to: Set up multiple PCR..

DNA Ligation

Following restriction enzyme digestion of DNA molecules, researchers need to rejoin the ends of the DNA to generate recombinant DNA, a process known as ligation.  Ligation of DNA is achieved with..

DNA Restriction Digestion Analysis

Restriction enzymes are DNA-cutting enzymes found in bacteria and as they cut within the molecule, are often called restriction endonucleases. A restriction enzyme recognizes and cuts DNA only at a pa..

Expression of a Recombinant Protein

The final goal in molecular biology is often the expression of a recombinant protein. The transformed plasmids can be used as templates by the bacteria to produce protein.   Students learn ..

Genetic Defect Correction with Bacterial Transformation

A major goal of genetic engineering is to have the ability to correct genetic defects to treat genetic diseases, such as cystic fibrosis, sickle cell anemia and Huntington’s disease.  &n..

Introduction to Agarose Electrophoresis

Agarose gel electrophoresis is a routinely used tool for separating nucleic acids.    Nucleic acids are negatively charged molecules, which when loaded onto the solid agarose matrix, m..

Isolate Your Own Genomic DNA

Genomic DNA isolation is a crucial technique in molecular biology, genetic manipulation and biotechnology.  To carry out these exciting and innovative sciences, researchers require an original so..

Mutation Detection and Analysis

A PCR Based Laboratory Research Experiment.  A major challenge for molecular biologists and genetic engineers is to easily detect and analyze genetic mutations that occur naturally, causing disea..