Extraction and Lysis Systems

We offer a wide variety of products for protein extraction and lysis.

 

Our protein extraction and lysis buffer systems (PE LB™) ensure good protein recovery, while maintaining the biological activity of the proteins. The solubilized proteins are suitable for enzyme assays, electrophoresis, folding studies, chromatographic studies and many other downstream applications. The PE LB™ systems offer a wide selection of buffers for lysis and extraction of proteins from bacteria, yeast, mammalian cell cultures and tissues.

 

FOCUS™ Proteome Kits are for the preparation of total protein, including soluble, insoluble, membrane, cytoplasmic, nuclear, signal, phospho- and glyco- proteins. These kits are simple to use, save time, improve the quality of protein analysis and enhance the chances of discovery of novel proteins.  They are suitable for the analysis of proteins using electrophoresis and other biochemical techniques and are compatible with biological samples from tissues, cells, plants, yeast, bacteria, insects and biological samples.

 

Our lysis buffers and kits include RIPA lysis buffer, a complete lysis buffer; an inclusion bodies solubilzation buffer, IBS™ Buffer; kits for total protein extraction (TPE™) for the extraction of total proteins from cells and tissues, and an RBC lysis buffer specifically designed for the lysis of red blood cells.

 

Accessories are available to assist with protein extraction and isolation procedures which include our easy grinding resin, a highly efficient grinding resin with matching pestles and Molecular Grinding Resin™ which is available with matching pestles and tubes. We also offer ProteaseArrest™, a general and complete protease inhibitor cocktail solution.

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IBS-HP™ Buffer

The expression of recombinant proteins is a popular and routinely used technique in protein studies.  The expression of recombinant proteins often has one drawback and that is the recombinant pro..

2D-Xtract™

A suitable protein solubilization buffer for 2D analysis must solubilize proteins effectively, without disturbing the native charge of the proteins. Urea based solubilization buffers solubilize protei..

Bacterial PE LB™

Bacterial PE LB™ has been developed for the extraction of soluble proteins and inclusion bodies from bacterial cells. It is a proprietary improvement on the lysozyme based lysis, which allows ex..

Bacterial PE LB™ in Phosphate Buffer

Bacterial PE LB™ in Phosphate buffer is a variation of Bacterial PE LB™ buffers only in phosphate buffer. It is used preferably for extraction of soluble proteins and soluble recombinant p..

DTT

High quality proteomic grade DTT, in various sizes, is available for your convenience.     Dithiothreitol (DTT) DTT is a common reducing agent used for the cleavage of disulfide ..

EZ-Grind™

A highly efficient grinding resin that is pre-aliquoted into 1.5ml grinding tubes and is supplied with matching pestles. The resin is designed for optimal grinding of biological samples for the extrac..

FOCUS™ Bacterial Proteome

Specifically designed for bacterial research and supplied with bacteria specific reagents. Extracts and solubilizes nearly all of the proteins from E.coli, including membrane as well as soluble protei..

FOCUS™ Extraction Buffers

Chaotropic extraction buffers that preserve the native charge of proteins making them suitable for sample extraction for 2D gels   One of the most important considerations before running 2D..

FOCUS™ Insect Proteome

Extracts and solubilizes nearly all of the proteins from insect cell cultures (i.e. Sf9 and Sf21), including membrane as well as soluble proteins, using a strong chaotropic extraction buffer to solubi..

FOCUS™ Mammalian Proteome

Extracts and solubilizes nearly all of the proteins from mammalian samples, including membrane as well as soluble proteins, by a strong chaotropic extraction buffer to solubilize even the most difficu..

FOCUS™ Plant Proteome

Specifically designed for plant research and supplied with plant specific reagents, including reagents for removal of plant pigments and other natural products that may interfere with protein analysis..

FOCUS™ Yeast Proteome

Specifically designed for yeast research and supplied with yeast specific reagents. Extracts and solubilizes nearly all of the proteins from yeast, including membrane and soluble proteins.  Extra..