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Sample Preparation

Sample preparation is curcial to accurate and informative downstream analysis.. Frequently, the component of interest is present at levels too low for detection or in the presence of interfering matrix elements that may mask the analyte.  Properly selected sample preparation techniques can remove excess contaminants and concentrate the analyte to adequate levels for measurement.  Applied properly these techniques yield clean and informative samples.  G-Biosciences offer a variety of tools designed to remove interfering contaminants and concentrate your samples to desirable concentrations.

Dialysis techniques are reliable and cost effective methods for concentrating samples.  G-Biosciences created novel dialysis tools which allow for the concentration of samples with zero sample loss.  Tube-O-CONCENTRATOR™ is a versatile concentration tool which utilizes a novel, water-absorbing, liquid polymer and the patented Tube-O-DIALYZER™​ for rapid concentration of dilute sample solutions with zero analyte loss.  The Tube-O-CONCENTRATOR™ and Tube-O-DIALYZER™​ systems are designed for centrifugation.  The Tube-O-REACTOR™ is a system that allows key steps of cross-linking, coupling, and modification of proteins and/or nucleic acids for be performed in a single tube.  This tool minimizes the risk of sample loss and reduces expiremental time and hands-on steps.

Removal of interfering agents such as detergents, salts, dyes, and radioisotopes in an integral part of effective sample preparation.  For these purposes, G-Biosciences developed an array of spin columns which accomodate a variety of volumes and molecular weight ranges.  The SpinOUT™ GT-100SpinOUT™ GT-600, and SpinOUT™ GT-1200 columns are versatile, spin-format columns for the desalting and buffer exchange of peptide, protein, and nucleic acid samples ranging from 5ųL to 4mL sample volumes.  This technology is also available in a 96-well spin plate format.  Optimized for FPLC applications, the G-Trap™ line of desalting columns are ideal for buffer exchange and desalting of samples of a variety of volumes and MWCO’s.  G-Trap™ columns are available for three MWCO’s, 700Da G-Trap™ GT-100, 6kDa G-Trap™ GT-600, and 30kDa G-Trap™ GT-1200.

Preciptation of proteins is a common purification technique.  OrgoSol-PROTEIN-Concentrate™ precipitates protein without disrupting the native structure, retaining the activity of most proteins.  A Universal Protein Precipitation Agent (UPPA™) was developed for the concentration of protein solutions as dilute as 1ng/mL.  Protein precipitation is not affected by detergents, chaotropic agents, or other reagents when using the UPPA™ system and recovery is generally 100%.

 

Download the Protein Sample Preparation Handbook

G-Biosciences C18 Spin Columns are ready-to-use micro centrifuge columns for peptide clean up and concentration.  The columns consist of porous C18 reverse-phase resin that has a particle size if ~15µm and a pore size of 300Å.  The resin offers highly efficient binding and reco..
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The Column-PROTEIN-Concentrate™ kit has been specifically developed for concentration of those proteins that cannot be concentrated either by precipitation or other techniques. The kit is based on a proprietary Protein Binding Resin that binds and immobilizes any protein in a low salt buffer b..
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Concentrator Powder is a high molecular weight polymer which will not migrate across the dialysis membrane.  Simply transfer your dilute protein solution to a dialysis bag or dialysis device, such as our patented Tube-O-DIALYZER™ and then cover the membrane with Concentrator Powder. ..
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Concentrator Solution is a novel liquid polymer for the rapid concentration of dilute protein solutions with zero loss, using dialysis.   Simply transfer your dilute protein solution to a dialysis bag or dialysis device*, such as our patented Tube-O-DIALYZER™ and dialyze against the con..
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DeSalting Columns are ready-to-use disposable columns for separating macromolecules, i.e. proteins, from low molecular weight buffer salts and reagents. See our SpinOUT™ product line for rapid desalting of proteins >6 or >30kDa...
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Dialysis is the process of separating molecules in solution by the difference in their rates of diffusion through a semi permeable membrane, such as dialysis tubing or Tube-O-DIALYZER™ dialysis caps. Molecules small enough to pass through the dialysis membrane move across the membrane in ..
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EndotoxinOUT™ was designed for the rapid removal of endotoxins and pyrogens (LPS, lipopolysaccharides) from nucleic acid (DNA) samples. EndotoxinOUT™ is an ideal product for the cleanup of buffers, cell culture media, protein solutions, nucleic acid (DNA) samples and pharmacological comp..
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G-Trap™ GT-1200 Desalting Column is prepacked ready to use column for separation of low molecular weight substances from high molecular weight substances based on size exclusion chromatography.    G-Trap™ GT-1200 Desalting Columns are mostly used for removal of salt or buffer..
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The OrgoSol-PROTEIN-Concentration™ kit precipitates protein with a proprietary solvent buffer, OrgoSol™. The OrgoSol™ buffer has been specifically developed for efficient precipitation of protein solutions with minimal disruption to the protein structure and therefore maintains the..
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PAGE-Optimizer™ is a unique, spin column for the preparation of samples for SDS-PAGE.  The columns contain a proprietary separation matrix that removes contaminants such as salts, detergents, cellular agents and other common buffering agents.     PAGE-Optimizer™ helps el..
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Remove Electrophoretic Contaminants for Improved Protein Resolution and Publication Quality Gels; Ideal for Difficult or Dilute Samples   Many lysis buffers and reagents used in sample preparation are incompatible with routinely used electrophoretic analysis. The presence of contaminants, or ..
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Sputo-LR™ is a sputum liquefying reagent for sputum and other mucoid fluids that is useful for the isolation of pathogenic or saprophytic bacteria, fungi, yeast, and epithelial cells. Sputo-LR™ is a concentrated sterile solution of Cleland’s Reagent (dithiothreitol, DTT) in phospha..
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