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Gel Filtration/ Size Exclusion

Size exclusion chromatography (SEC) or gel filtration is used to separate a wide range of molecules according to size, including proteins (enzymes), polysaccharides and nucleic acids. 

There are two major categories of SEC; Group separation and Fractionation.

In group separation, for example desalting, samples are separated into two major groups. For desalting select a resin that excludes the larger molecules from the pores of beads, whiles smaller molecules (salts) are retained in the pores.

Fractionation is used to separate macromolecules of different sizes. Here the fractionation range of the resin defines the range of molecular weights that can be separated. Applications that use fractionation by SEC include, preparative purification, analysis of aggregates and molecular weight determination of proteins and nucleic acids.

Download the Protein Purification Handbook for a full list of related products

Download the Purification Resins Handbook for Complete Product List

G-Sep™ is a gel filtration resin comprised of ultrapure cross-linked dextran for desalting and buffer exchange in industrial applications. It exhibits high selectivity, superb resolution, low non-specific adsorption and robust chemical stability.   Quickly desalts, removes contaminants..
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G-Sep™ is a gel filtration resin comprised of ultrapure cross-linked dextran for desalting and buffer exchange in industrial applications. It exhibits high selectivity, superb resolution, low non-specific adsorption and robust chemical stability. Quickly desalts, removes contaminants and tr..
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G-Sep™ Agarose 4B is a gel filtration matrix formed from agarose beads that is available with 4% agarose content, designated G-Sep™ Agarose 4B.   The resin is a proven gel filtration base matrix and is routinely modified by researchers to couple affinity ligands.  The resin i..
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G-Sep™ Agarose 6B is a gel filtration matrix formed from agarose beads that is available with 6% agarose content, designated G-Sep™ Agarose 6B.   The resin is a proven gel filtration base matrix and is routinely modified by researchers to couple affinity ligands.  The resin i..
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G-Sep™ Agarose CL-4B resin is a cross-linked versions of our G-Sep™ Agarose. Cross-linking of the agarose results in chemically and physically more stable agarose beads that offer the same selectivity, but with better flow characteristics. Cross-linked agarose beads are resistant to..
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G-Sep™ Agarose CL-6B resin is a cross-linked versions of our G-Sep™ Agarose.  Cross-linking of the agarose results in chemically and physically more stable agarose beads that offer the same selectivity, but with better flow characteristics. Cross-linked agarose beads are r..
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G-Sep™ Agarose 6 Fast Flow (FF) is a gel filtration matrix formed from agarose beads that is based on our cross-linked 6% agarose.    The modification to the cross-linked 6% agarose to fast flow results in improved physical stability and chromatographic qualities.  The modific..
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G-Trap™ GT-100 Desalting Column is prepacked ready to use column for separation of low molecular weight substances from high molecular weight substances based on size exclusion chromatography.    G-Trap™ GT-100 Desalting Columns are mostly used for removal of salt or buffer e..
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G-Trap™ GT-1200 Desalting Column is prepacked ready to use column for separation of low molecular weight substances from high molecular weight substances based on size exclusion chromatography.    G-Trap™ GT-1200 Desalting Columns are mostly used for removal of salt or buffer..
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G-Trap™ GT-600 Desalting Column is prepacked ready to use column for separation of low molecular weight substances from high molecular weight substances based on size exclusion chromatography.    G-Trap™ GT-600 Desalting Columns are mostly used for removal of salt or buffer e..
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Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.    The SpinOUT™ GT-100, GT-600 and GT-1200 columns are versatile, spin-format columns for the desalting and buffer exchange of peptide, protein ..
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The SpinOUT™ G-Acryl columns are versatile, spin-format columns for the desalting and buffer exchange of peptide and protein and other macromolecule solutions ranging from 5µl through to 3ml sample volumes.  The SpinOUT™ columns are available in three MWCO sizes for >1,800,..
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