Gel Filtration/ Size Exclusion

Size exclusion chromatography (SEC) or gel filtration is used to separate a wide range of molecules according to size, including proteins (enzymes), polysaccharides and nucleic acids. 

There are two major categories of SEC; Group separation and Fractionation.

In group separation, for example desalting, samples are separated into two major groups. For desalting select a resin that excludes the larger molecules from the pores of beads, whiles smaller molecules (salts) are retained in the pores.

Fractionation is used to separate macromolecules of different sizes. Here the fractionation range of the resin defines the range of molecular weights that can be separated. Applications that use fractionation by SEC include, preparative purification, analysis of aggregates and molecular weight determination of proteins and nucleic acids.

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G-Sep™ Agarose 4B

G-Sep™ Agarose 4B is a gel filtration matrix formed from agarose beads that is available with 4% agarose content, designated G-Sep™ Agarose 4B.   The resin is a proven gel filtr..

G-Sep™ Agarose 6B

G-Sep™ Agarose 6B is a gel filtration matrix formed from agarose beads that is available with 6% agarose content, designated G-Sep™ Agarose 6B.   The resin is a proven gel filtr..

G-Sep™ Agarose CL-4B

G-Sep™ Agarose CL-4B resin is a cross-linked versions of our G-Sep™ Agarose. Cross-linking of the agarose results in chemically and physically more stable agarose beads that offer the..

G-Sep™ Agarose CL-6B

G-Sep™ Agarose CL-6B resin is a cross-linked versions of our G-Sep™ Agarose.  Cross-linking of the agarose results in chemically and physically more stable agarose beads tha..

G-Sep™ Agarose Fast Flow

G-Sep™ Agarose 6 Fast Flow (FF) is a gel filtration matrix formed from agarose beads that is based on our cross-linked 6% agarose.    The modification to the cross-linked 6% agar..

G-Trap™ GT-100 Desalting Column

G-Trap™ GT-100 Desalting Column is prepacked ready to use column for separation of low molecular weight substances from high molecular weight substances based on size exclusion chromatography.&n..

G-Trap™ GT-1200 Desalting Column

G-Trap™ GT-1200 Desalting Column is prepacked ready to use column for separation of low molecular weight substances from high molecular weight substances based on size exclusion chromatography.&..

G-Trap™ GT-600 Desalting Column

G-Trap™ GT-600 Desalting Column is prepacked ready to use column for separation of low molecular weight substances from high molecular weight substances based on size exclusion chromatography.&n..