Reducing Reagents

The reduction of disulfide bridges in protein is a routinely used technique in proteomics and is involved in protein denaturation, solubilization, protection of protein thiols, characterization of protein (disulfide bonds), separation and study of protein subunits, and protein activation for cross-linking.

Numerous protein reductants, including DTT, ß-mercaptoethanol and TCEP (Tris (2-carboxyethyl) phosphine) are offered in different formats, including powders, solutions and OneQuant™ formats.

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β-Mercaptoethanol

High quality proteomic grade β-mercaptoethanol.   β-mercaptoethanol, a popular reducing agent, is offered in 5ml and 100ml bottles.   Features Synonym: &be..

DTT

High quality proteomic grade DTT, in various sizes, is available for your convenience.     Dithiothreitol (DTT) DTT is a common reducing agent used for the cleavage of disulfide ..

Ellman’s Reagent

5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB)   Ellman's reagent is a versatile, water-soluble compound for quantifying free sulfhydryl groups in solution. It reacts with a free sulf..

Hydroxylamine·HCl

Hydroxylamine•HCl is a reducing agent that is routinely used for the deacetylation of SATA to form free sulfhydryls (Figure 1), for cleavage of protein cross-linkers that contain carbonyl groups ..

Immobilized Reductant

Reducing agents are used in the reduction of disulfide bonds of proteins and peptides.  It is often necessary to remove the reducing agents from the protein/peptide solutions to prevent them from..

Protein-S-S-Reductant™

A water soluble, odorless, non-toxic and stable protein reductant. Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to ß-mercaptoethanol and DTT (d..

Sodium Cyanoborohydride

Carbonyl groups, including aldehydes, ketones and glyoxals, react with amines to form Schiff base intermediates that are in equilibrium with their free forms.  The labile Schiff’s base inte..

TCEP-HCl; Tris (2-carboxymethyl) phosphine-HCl

TCEP is a water-soluble, odorless, non-toxic and stable protein reductant. This potent reducing agent is a popular alternative to ß-mercaptoethanol and DTT (dithiothreitol) use for most applicat..