Mitochondria are referred to as “power-houses of cell” as they generate the majority of cellular ATP via oxidative phosphorylation. Furthermore, ß-oxidation of short-, medium and long- chain fatty acids is carried in mitochondria and mitochondria are involved in ROS generation and apoptosis. Mitochondria also play a key role in intermediary metabolism.
Mitochondrial dysfunction has been linked to variety of market therapeutics and also to several pathological diseases. Therefore studying or checking mitochondrial health is paramount to cell health.
FCCP [Carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone] is a potent uncoupler of oxidative phosphorylation in mitochondria. It is used as a positive control for mitochondrial membrane depolarizat..