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Protein Sample Preparation

Download the Protein Electrophoresis Handbook for Full Product ListDownload the Protein Sample Preparation Handbook

 

G-Biosciences provide a range of sample preparation reagents to insure your protein samples have excellent resolution and publication quality gels. A two-step method for sample cleaning, PAGE-Perfect™, removes electrophoresis interfering agents including detergents, salts, chaotropes, reducing agents and sugars to improve gel quality and reproducibility. The 2D electrophoresis optimized, Perfect-FOCUS™ reagent is ideal for producing streak free, high spot resolution gels. We carry a range of reductant reagents such as DTT, Protein-S-S-Reductant™, ß-Mercaptoethanol and TCEP-HCl; Tris (2-carboxyethyl) phosphine-HCl which are high quality, proteomic grade and available in several convenient quantities.

1,4 Dithiothreitol (DTT) is a small molecule reducing agent, also known as Cleland’s reagent. G-Biosciences DTT is a high quality proteomic grade DTT and is supplied in various sizes, including a OneQuant™ version. The OneQuant™ format features 40 vials of 7.7mg DTT for conven..
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Reducing agents are used in the reduction of disulfide bonds of proteins and peptides.  It is often necessary to remove the reducing agents from the protein/peptide solutions to prevent them from interfering with subsequent procedures.  However, for small proteins, and particularly peptide..
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LDS Sample Loading Buffer [4X] is used to prepare protein samples for SDS polyacrylamide gel electrophoresis.   Features Alkaline pH (pH 8.4) for optimal conditions for denaturation and reduction of disulfide bonds 4X concentrated allows for larger sample loading It has Lithium Dodecyl S..
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PAGE-Optimizer™ is a unique, spin column for the preparation of samples for SDS-PAGE.  The columns contain a proprietary separation matrix that removes contaminants such as salts, detergents, cellular agents and other common buffering agents.     PAGE-Optimizer™ helps el..
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Remove Electrophoretic Contaminants for Improved Protein Resolution and Publication Quality Gels; Ideal for Difficult or Dilute Samples   Many lysis buffers and reagents used in sample preparation are incompatible with routinely used electrophoretic analysis. The presence of contaminants, or ..
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A water soluble, odorless, non-toxic and stable protein reductant. Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to ß-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider ..
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A reagent kit for detection and estimation of SDS in a sample. Mix the test sample in the extraction buffer reagents provided with the kit. If SDS is present in the sample, a blue color is extracted that can be quantitatively measured.   The working range of the assay is 0.02-0.2% SDS...
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The TPE™ kit is a universal lysis system designed for the solubilization and extraction of total proteins from cells and tissues for SDS-PAGE Analysis.  The kit is suitable for animal, plant, yeast, bacteria, and other biological samples.   The TPE™ kit provides a two compo..
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TCEP is a water-soluble, odorless, non-toxic and stable protein reductant. This potent reducing agent is a popular alternative to ß-mercaptoethanol and DTT (dithiothreitol) use for most applications. The reduction potency of TCEP is twice as high as that of DTT, and TCEP is effective in reduci..
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High quality proteomic grade β-mercaptoethanol.   β-mercaptoethanol, a popular reducing agent, is offered in 5ml and 100ml bottles.   Features Synonym: β-Mercaptoethanol CAS#: 60-24-2 Molecular Formula: C2H6OS Molecular Weight: 78.13   Applications ..
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G-Biosciences Immobilized Soybean Trypsin Inhibitor (STI) resin is designed for the efficient removal of trypsin, chymotrypsin and elastase proteases from protein digests. The action of the Immobilized STI resin will stop enzymatic reactions, in addition to removing the proteases and simplifying the..
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