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DMF

Anhydrous DMF [N,N-Dimethylformamide (HCON(CH3)2)]. Suitable for cross-linking, protein modification and biotinylation reaction applications...

HABA

A biotin estimation dye reagent...

Optimizer Buffer™

The conjugation and modification reactions used to cross-link proteins or couple labels to proteins, such as biotin, enzymes, and fluorescent dyes, require certain conditions, including pH and chemica..

Avidin

Avidin is a glycoprotein with approximately 10% of its total mass comes from carbohydrates. Avidin has a molecular weight of 67kDa and contains four identical 128 amino acid subunits that each have a ..

DMSO

Anydrous DMSO [Dimethyl sulfoxide (CH3)2SO] is offered for the solubilization of the water insoluble cross-linkers and modification reagents.     ..

Hydroxylamine·HCl

Hydroxylamine•HCl is a reducing agent that is routinely used for the deacetylation of SATA to form free sulfhydryls (Figure 1), for cleavage of protein cross-linkers that contain carbonyl groups ..

L-Cysteine•HCl monohdyrate

L-Cysteine hydrochloride salt is routinely used with Ellman's Reagent assays as a sulfhydryl standard. In addition, it is also used as a supplement for protein refolding experiments.   ..

Pearl™ Antibody Clean Up Kit

Purified and commercial antibodies are routinely stored in buffers containing bovine serum albumin (BSA) and gelatin that act as stabilizers during long term storage. In routine applications, suc..

Sodium meta-Periodate

Sodium metaperiodate, or sodium m-periodate, is a mild oxidant that is routinely used for the conversion of cis-glycol groups in carbohydrates to reactive aldehdye groups (Figure 1).  The reactiv..

SpinOUT™ Columns

Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.    The SpinOUT™ GT-100, GT-600 and GT-12..

Tube-O-Reactor™

Tube-O-Reactor™ is a system that allows all the key steps of cross linking, coupling and modification of proteins and/or nucleic acids to be performed in a single tube. This minimizes the risk o..

Well-Coated™ Biotin

Well-Coated™ Biotin plates are designed to specifically bind avidin, streptavidin or Neutravidin™ conjugated molecules, including enzyme conjugates.    Biotin exhibits an e..