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Apoptosis Assays & Accessories

Our Apoptosis Assays & Accessories section includes a wide variety of research products.

A wide range of apoptosis assays and accesories are available including Apoptotic DNA Ladder, caspase substrates and caspase uninduced lysates.
 
A selection of apoptosis inducers including caspase inhibitors are also available.
4-Vinylpyridine is used as a derivatizing reagent for free thiols such as GSH. It is used in the Glutathione colorimetric assay to remove reduced GSH, so that the oxidized Glutathione (GSSG) concentration can be measured. 4-Vinylpyridine is a better derivatizing reagent for GSH when compared to N-et..
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A class of polypeptide antibiotics isolated from the Steptomyces soil bacteria.  Actinomycin D inhibits transcription by binding to DNA at the transcription initiation complex and therefore preventing elongation by the RNA polymerase.   Supplied as 50μl 10mM solution.   ..
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Apoptosis is a form of programmed cell death which is essential as a normal part of development in multicellular organisms. Apoptosis go awry in some disease states such as Alzheimer’s and cancer.   Annexin V Apoptosis Assay contains fluorescent dye conjugated Annexin V  which&..
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Gene/Protein:
The Apoptotic DNA Ladder kit contains all of the reagents necessary for the preparation of cellular fractions for the production of apoptosis nucleosomal DNA ladders. The kit does not require the use of toxic phenol. The protocol involves: cell lysis; removal of cellular debris; and precipitation of..
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Ready-to-use high quality apoptosis inducers allow users an easy tool for induction of apoptosis in cultured cells. These stabilized reagent solutions have a long storage life and are easy to use. The selection of apoptosis inducers include actinomycin D, camptothecin, cycloheximide, dexamethasone, ..
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4-ethyl-4-hydroxy-1H-pyrano[3’,4’:6,7]indolizino[1,2-b] quinoline-3,14-(4H,12H)-dione     A cytotoxic quinoline alkaloid that inhibits the DNA enzyme topoisomerase I (Topo I). Supplied as 1ml 2mM   Features Synonym: CPT CAS Number: 7689-03-4 Linear Formula: C20H1..
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(4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione)     An inhibitor of protein synthesis by interfering with the translocation step (movement of two tRNA molecules and mRNA on ribosome) in protein synthesis, thus blocking translational elongati..
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((8S,10S)-10-(4-amino-5-hydroxy-6-methyl-tetrahydro-2H-pyran-2-yloxy)-6,8,11-trihydroxy-8-(2-hydroxyacetyl)-1-methoxy-7,8,9,10-tetrahydrotetracene-5,12-dione)     Doxorubicin (or hydroxyldaunorubicin) is an anthracycline antibiotic that intercalates DNA, inhibiting the unwinding of DNA b..
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(4’-demethyl-epipodophyllotoxin 9-[4,6-O-(R)-ethylidene-beta-D-glucopyranoside], 4’ -(dihydrogen phosphate))     An inhibitor of topoisomerase II.     Supplied as 50μl 100mM solution.  ..
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FCCP [Carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone] is a potent uncoupler of oxidative phosphorylation in mitochondria. It is used as a positive control for mitochondrial membrane depolarization. FCCP disrupts the synthesis of ATP by trnasporting protons across mitochondrial inner membrane..
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Gene/Protein:
Reduced glutathione (L-γ-glutamyl-L-cysteinylglycine), a tripeptide, serves as a key antioxidant in animal, plant, fungi and bacteria by providing free thiol. Glutathione exist in reduced (GSH) and oxidized (GSSG; gluthathione disulphide) forms in cells and tissues, and the concentration of gl..
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JC-1 Mitochondrial Membrane Potential Assay kits comprise lipophilic cationic fluorescent dye; 5,5,6,6’-tetrachloro-1,1’,3,3’-tetraethylbenzimi-dazoylcarbocyanine iodide (JC-1).  In healthy cells, JC-1 enters the energized mitochondria and form aggregates which change the fluo..
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