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Cell Toxicity & Proliferation Assays

Download the Bioassay Handbook & Selection Guide for Complete Product Listing

 

G-Biosciences offers a wide range of non-radioactive bioassays for the detection of cell toxicity, cell death and cell proliferation that are ideal for high throughput screening.  Each CytoScan™ assay is designed for a microwell format and is suitable for studying chemical and cell-mediated cytotoxicity, viability, death or proliferation.
 

AlamarBlue Cell Viability Assay reagent quantitatively measures the proliferation of mammalian cell lines, bacteria and fungi. The dye in the Alamar blue assay incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and changes color in response to the chemical reduction of growth..
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Calcein AM or Calcein acetoxymethyl ester is a hydrophobic compound which passes easily through cell membrane into live cells and is used for cell viability assays. The non-fluorescent calcein AM dye is hydrolyzed by cellular esterases to give calcein which is fluorescent and is retained in the cyto..
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CytoScan™-SRB Cytotoxicity Assay is an accurate and reproducible assay based upon the quantitative staining of cellular proteins by sulforhodamine B (SRB).   This assay has been used for high-throughput drug screening at the National Cancer Institute (NCI) (1). Sulforhodamine B is an ..
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CytoScan™ WST-1 Cell Proliferation Assay is a sensitive and accurate assay for cell proliferation and cytotoxicity. The assay is highly convenient as it is performed in a single cell tissue culture well and requires no washing, harvesting or solubilization of cells.     Adherent or..
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The CytoScan™-fluoro is based on the quantification of cellular lactate dehydrogenase (LDH) released when cells are damaged or under stress. Lactate dehydrogenase released into the culture medium is measured with a diaphorase coupled enzymatic assay that results in the conversion of a non-fluo..
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Reduced glutathione (L-γ-glutamyl-L-cysteinylglycine), a tripeptide, serves as a key antioxidant in animal, plant, fungi and bacteria by providing free thiol. Glutathione exist in reduced (GSH) and oxidized (GSSG; gluthathione disulphide) forms in cells and tissues, and the concentration of gl..
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Firefly luciferase is a unique photoprotein used in bioluminescence for high sensitive detection and quantification of ATP and as a reporter for studying gene function and regulation.  Luciferase (Firefly, Recombinant) is recombinant 62 kDa protein expressed in E. Coli.   Click here to re..
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ATP serves as a marker for cell viability as it is present in all metabolically active cells. Lumino™ Cell Viability Glow Assay is a bioluminescent assay to detect and/or quantify ATP and thus measures the viable cells. The bioluminescent assay is based on requirement of ATP by lucif..
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*Pluronic® F-127 is a nonionic, surfactant polyol with molecular weight around 12,500 daltons. Pluronic® F-127 promotes solubilization of water-insoluble dyes and other materials in physiological medium. Pluronic® F-127 is most commonly used to disperse the acetomethyl (AM) esters as w..
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• X-Gal conveniently aliquoted into 4 separate vials to prolong shelf-life! • 4 x 100 assays! • 200ml assay volume! • Pre-optimized!  • Works on adhered/suspended cells or frozen tissue sections! Senescence kit is designed to be more convenient and cost efficient t..
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Calcein AM or Calcein acetoxymethyl ester is a hydrophobic compound which passes easily through cell membrane into live cells and is used for cell viability assays. The non-fluorescent calcein AM dye is hydrolyzed by cellular esterases to give calcein which is fluorescent and is retained in the cyto..
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A reliable colorimetric assay that measures the stable, cytosolic, lactate dehydrogenase (LDH) enzyme, which is released from damaged cells and is a biomarker for cellular cytotoxicity and cytolysis. The released LDH is measured with a coupled enzymatic reaction that results in the conversion of a t..
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