Antibody Purification Resins

Affinity chromatography plays a major role in their production of antibodies and antibody-based products. The basis for antibody affinity chromatography is the high affinity and specificity of protein A and protein G for the Fc-region of IgG from a variety of species. 

Protein A and G immobilized on chromatography media and are highly effective in isolating subclasses of IgG from ascites fluid, cell culture supernatants, and serum. Protein A and protein G differ in their ability to bind antibodies of different species and subclasses, and are widely used for the routine purification of antibodies.

G-Biosciences Pearl Resin is also offered as a one-step purification of IgGs from serum.

Resins for IgM, IgY and IgA are also available.

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IgG Binding, Wash & Elution Buffers

G-Biosciences IgG Binding, Wash & Elution Buffers are designed for the non-denaturing, high yield purification of antibodies from IgG affinity purification resins.  The buffers are ideal for ..

Immobilized Jacalin

Jacalin, or Artocarpus integrifolia lectin, is a tetrameric two-chain lectin with a molecular weight of 66kDa. Jacalin is a α-D-galactose binding lectin purified from jack-fruit (Artocarpus int..

Pearl™ Antibody Clean Up Kit

Purified and commercial antibodies are routinely stored in buffers containing bovine serum albumin (BSA) and gelatin that act as stabilizers during long term storage.  In routine applications, su..

Pearl™ Monoclonal IgG Purification Kit

The Pearl™ Monoclonal IgG Purification kit allows for the rapid purification of antibodies from cell culture supernatant and ascites fluid. The Pearl™ IgG Purification Resin binds the high..

Protein A Magnetic Beads

G-Biosciences' Protein A Magnetic Beads are Fe3O4 beads coated with dextran to produce highly uniform, 1µm beads. Recombinant Protein A is covalently coupled to the dextran coat to produce an en..

Protein G Magnetic Beads

G-Biosciences' Protein G Magnetic Beads are Fe3O4 beads coated with dextran to produce highly uniform, 1µm beads. Recombinant Protein G is covalently coupled to the dextran coat to produce ..

Thiophilic Adsorption Kit

Thiophilic adsorption or thiophilic chromatography is a routinely used technique for the low cost, simple purification of immunoglobulins.  Thiophilic adsorption was first developed by Porath e..

Thiophilic Resin

Thiophilic adsorption or thiophilic chromatography is a routinely used technique for the low cost, simple purification of immunoglobulins.  Thiophilic adsorption was first developed by Porath et ..

G-Trap™ Protein G

G-Trap™ Protein G are prepacked ready to use columns for affinity purification of Immunoglobulins G. The column is packed with resin, which is covalently coupled to recombinant Protein G. ..

G-Trap™ rProtein A FF

G-Trap™ rProtein A FF are prepacked ready to use columns for purification of IgG antibodies.   The column is packed with fast flow resin, which is covalently coupled to recombinant Pr..

Immobilized Protein A

Immobilized Protein A is for binding the constant domains of immunoglobulin (Ig) molecules (see Figure 1). Protein A is coupled to agarose beads by reductive amination method that provides high c..

Immobilized Protein A/G

Immobilized Protein A/G consists of recombinant protein A/G ligand covalently immobilized onto 6% highly cross-linked agarose.  The dynamic binding capacity will vary depending on several factors..