Affinity chromatography plays a major role in their production of antibodies and antibody-based products. The basis for antibody affinity chromatography is the high affinity and specificity of protein A and protein G for the Fc-region of IgG from a variety of species.
Protein A and G immobilized on chromatography media and are highly effective in isolating subclasses of IgG from ascites fluid, cell culture supernatants, and serum. Protein A and protein G differ in their ability to bind antibodies of different species and subclasses, and are widely used for the routine purification of antibodies.
G-Biosciences Pearl Resin is also offered as a one-step purification of IgGs from serum.
Immobilized protein G is for binding the constant domains of immunoglobulin (Ig) molecules (see Figure 1). It is ideal for the easy one-step purification of classes, subclasses and fragments of immuno..