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Premade Western Blots

Download the Protein Discovery Handbook for full list of lysates & blots

 

The premade blots are prepared with the GenLysate and 50μg protein is loaded onto a 4-20% denaturing polyacrylamide gel, along with a prestained molecular weight marker.  After the proteins are resolved they are transferred to a PVDF membrane, using G-Biosciences EfficientWestern Transfer Buffer.
The blots are ready to be blocked and probed with the antibodies of choice.
Note: We recommend that customers contact our technical department for the latest information on the blots as G-Biosciences reserves the right to change the blot profile due to the availability of GenLysate.

 

G-Biosciences blots are designed for discovery and as a result the blots are overloaded with 50ug protein. When extracting the proteins, G-Biosciences does not perform clean up steps to eliminate the risk of removing proteins that researchers are interested in.  The proteins are extracted with a simple detergent-based lysis buffer and protease inhibitors and then loaded on the gels.  This, for some tissues, may result in the appearance of poor migration and protein separation.

 

If a protein of interest is identified on these blots then G-Biosciences clean up products can be used on the equivalent Genlysate or a custom request can be made to G-Biosciences to clean the samples.

The blot is prepared using total protein extracted from a variety of 5-10 day old, whole plant tissues.    The premade blots are prepared with the appropriate GenLysate and 50μg protein is loaded onto a 4-20% denaturing polyacrylamide gel, along with a prestained molecular weight mark..
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The blot is prepared using total protein extracted from a variety of species, where whole species is not available the whole liver lysate is used.  These blots can be used to identify similar proteins in other species, including human, chicken, frog, worm, drosophila, yeast, aspergillus, E. col..
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Western ReProbe™ is a single component system that is specifically formulated to dissociate and remove antibodies from membrane-bound proteins without destroying the antigenic binding affinity. Western ReProbe™ also enables the ability to reuse western blots. Western ReProbe™ brea..
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