The premade blots are prepared with the GenLysate™ and 50μg protein is loaded onto a 4-20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved they are transferred to a PVDF membrane, using G-Biosciences Efficient™ Western Transfer Buffer.
The blots are ready to be blocked and probed with the antibodies of choice.
Note: We recommend that customers contact our technical department for the latest information on the blots as G-Biosciences reserves the right to change the blot profile due to the availability of GenLysate™.
G-Biosciences blots are designed for discovery and as a result the blots are overloaded with 50ug protein. When extracting the proteins, G-Biosciences does not perform clean up steps to eliminate the risk of removing proteins that researchers are interested in. The proteins are extracted with a simple detergent-based lysis buffer and protease inhibitors and then loaded on the gels. This, for some tissues, may result in the appearance of poor migration and protein separation.
If a protein of interest is identified on these blots then G-Biosciences clean up products can be used on the equivalent Genlysate or a custom request can be made to G-Biosciences to clean the samples.