Beta-Galactosidase Assays

β-galactosidase, also called beta-gal or β-gal, is a hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides. Substrates of different β-galactosidases include ganglioside GM1, lactosylceramides, lactose, and various glycoproteins. The β-galactosidase gene functions well as a reporter gene for two major reasons: its protein product is extremely stable and resistant to proteolytic degradation in cellular lysates, and most importantly, the enzyme is easily assayed. 

 

G-Biosciences offers highly sensitive colorimetric and fluorescent assays for the reporter gene β-galactosidase.

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β-Galactosidase Assay (CPRG)

β-Galactosidase Assay (CPRG) provides an easy, rapid, and highly sensitive method for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase..

2-Naphthyl-beta-D-galactopyranoside

2-Naphthyl-β- D-galactopyranoside β-Naphthyl-β-galactoside; β-Nap-β-D-Gal   MOLECULAR BIOLOGY GRADE   White to off-white crystalline powder &nbs..

4-Aminophenyl-beta-D-galactopyranoside

4-Aminophenyl-β-D-gal   MOLECULAR BIOLOGY GRADE   4-Aminophenyl-β-D-galactopyranoside is a substrate for β-galactosidase. It is also a weak β-D-galactosidase ..

4-Methylumbelliferyl-beta-D-galactopyranoside

4-Methylumbelliferyl-β- D-galactopyranoside 4-MU-β-D-Gal   MOLECULAR BIOLOGY GRADE   White to off-white powder   Fluorogenic substrate for β-D-galact..

4-Nitrophenyl-beta-D-galactopyranoside

4-Nitrophenyl-β-D- galactosidase p-Nitrophenyl-β-D-galactoside PNP-β-D-Gal   MOLECULAR BIOLOGY GRADE   White to off white crystalline powder   Chr..

5-Bromo-3-indoxyl-beta-D-galactopyranoside

Bluo-gal is used as an alternative to X-gal that produces a darker blue color. Bluo-Gal is hydrolyzed by β-galactosidase to produce a blue precipitate. This precipitate is insoluble in alcohol an..

5-Bromo-6-chloro-3-indoxyl-beta-D-galactopyranoside

Magenta-gal is used as an alternative to X-gal that produces a red color. Magenta-Gal is hydrolyzed by β-galactosidase to produce a red precipitate. This precipitate is insoluble in alcohol and x..

Fluorescent β Galactosidase Assay (MUG)

The Fluorescent β-Galactosidase Assay (MUG) is a highly sensitive, fluorescent assay for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosid..

N-Methylindoxyl-beta-D-galactopyranoside monohydrate

N-Methylindoxyl-β-D- galactopyranoside GreenTM-β-D-galactoside 1-Methyl-3-indoxyl-β-D-galactopyranoside GreenTM-β-D-Gal   MOLECULAR BIOLOGY GRADE   O..

Phenyl-beta-D-galactopyranoside

Phenyl-β-D- galactopyranoside Phenyl-β-D-galactoside Ph-β-D-Gal   MOLECULAR BIOLOGY GRADE   White to off white crystalline powder   Phenyl-β-..

Senescence Histochemical Staining Kit

• X-Gal conveniently aliquoted into 4 separate vials to prolong shelf-life! • 4 x 100 assays! • 200ml assay volume! • Pre-optimized!  • Works on adhered/suspe..

X-Gal (5-Bromo-4-chloro-3-indoxyl-beta-D-galactopyranoside)

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside is most commonly known as X-Gal. X-gal is a histochemical substrate for β-galactosidase which cleaves X-Gal to yield an insoluble blue precip..