Beta-Galactosidase Assays
β-galactosidase, also called beta-gal or β-gal, is a hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides. Substrates of different β-galactosidases include ganglioside GM1, lactosylceramides, lactose, and various glycoproteins. The β-galactosidase gene functions well as a reporter gene for two major reasons: its protein product is extremely stable and resistant to proteolytic degradation in cellular lysates, and most importantly, the enzyme is easily assayed.
G-Biosciences offers highly sensitive colorimetric and fluorescent assays for the reporter gene β-galactosidase.
4-Methylumbelliferyl-β- D-galactopyranoside
4-MU-β-D-Gal
MOLECULAR BIOLOGY GRADE
White to off-white powder
Fluorogenic substrate for β-D-galactosidase, yielding a blue fluorescent solution upon cleavage.
Store at -15°C in the dark.
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The Fluorescent β-Galactosidase Assay (MUG) is a highly sensitive, fluorescent assay for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct
The study of the lac operon has played an important role in unders..
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• X-Gal conveniently aliquoted into 4 separate vials to prolong shelf-life!
• 4 x 100 assays!
• 200ml assay volume!
• Pre-optimized!
• Works on adhered/suspended cells or frozen tissue sections!
Designed to be more convenient and cost efficient than other k..
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β-Galactosidase Assay (CPRG) provides an easy, rapid, and highly sensitive method for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct. The β-Galactosidase Assay (CPRG) assay uses chlorophenol red-&beta..
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