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Beta-Galactosidase Assays

Download the Bioassay Handbook & Selection Guide for Complete Product Listing

 

β-galactosidase, also called beta-gal or β-gal, is a hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides. Substrates of different β-galactosidases include ganglioside GM1, lactosylceramides, lactose, and various glycoproteins. The β-galactosidase gene functions well as a reporter gene for two major reasons: its protein product is extremely stable and resistant to proteolytic degradation in cellular lysates, and most importantly, the enzyme is easily assayed. 

 

G-Biosciences offers highly sensitive colorimetric and fluorescent assays for the reporter gene β-galactosidase.

4-Methylumbelliferyl-β- D-galactopyranoside 4-MU-β-D-Gal   MOLECULAR BIOLOGY GRADE   White to off-white powder   Fluorogenic substrate for β-D-galactosidase, yielding a blue fluorescent solution upon cleavage.    Store at -15°C in the dark.  ..
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The Fluorescent β-Galactosidase Assay (MUG) is a highly sensitive, fluorescent assay for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct   The study of the lac operon has played an important role in unders..
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• X-Gal conveniently aliquoted into 4 separate vials to prolong shelf-life! • 4 x 100 assays! • 200ml assay volume! • Pre-optimized!  • Works on adhered/suspended cells or frozen tissue sections!   Designed to be more convenient and cost efficient than other k..
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β-Galactosidase Assay (CPRG) provides an easy, rapid, and highly sensitive method for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct.  The β-Galactosidase Assay (CPRG) assay uses chlorophenol red-&beta..
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