The rAmylase Project
The rAmylase Project is a unique set of ten self-contained laboratory experiments, kitted for teacher convenience. With The rAmylase Project Kits it is easy to pull together a unit, a semester, or a year-long course in biotechnology.
The kits can be used individually in a "plug'n'play" fashion to teach specific biotechnology techniques or, as a thematic unit that demonstrates how a commercially important biotechnology product, the amylase protein, is researched and manufactured. The rAmylase Project kits demonstrate that DNA is the flash but proteins are the cash of biotechnology.
- Characterizing Proteins by PAGE
- Assaying for Amylase Activity
- Direct ELISA of Bacterial α-Amylase
- Western Blot to Identify α-Amylase
- Determining the Concentration of Amylase in a Solution
- Restriction Digestion to Verify the pAmylase Plasmid
- Transformation of E. coli with pAmylase
- Using A Miniprep Kit for Plasmid Isolation
- Using Ion Exchange Chromatography to Separate Proteins
- Confirming the Amylase Gene Using PCR