The rAmylase Project

The rAmylase Project is a unique set of ten self-contained laboratory experiments, kitted for teacher convenience. With The rAmylase Project Kits it is easy to pull together a unit, a semester, or a year-long course in biotechnology.

The kits can be used individually in a "plug'n'play" fashion to teach specific biotechnology techniques or, as a thematic unit that demonstrates how a commercially important biotechnology product, the amylase protein, is researched and manufactured. The rAmylase Project kits demonstrate that DNA is the flash but proteins are the cash of biotechnology.

The rAmylase Project Kits
  1. Characterizing Proteins by PAGE
  2. Assaying for Amylase Activity
  3. Direct ELISA of Bacterial α-Amylase
  4. Western Blot to Identify α-Amylase
  5. Determining the Concentration of Amylase in a Solution
  6. Restriction Digestion to Verify the pAmylase Plasmid
  7. Transformation of E. coli with pAmylase
  8. Using A Miniprep Kit for Plasmid Isolation
  9. Using Ion Exchange Chromatography to Separate Proteins
  10. Confirming the Amylase Gene Using PCR

  

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Assaying for Amylase Activity (Lab 6C)

This kit is designed to teach 8 groups of four students the principles of enzyme assays.   The kit contains the reagents to allow students to test for the presence and activity of 2 differe..

Characterizing Proteins by PAGE (Lab 5F)

This kit is designed to teach 8 groups of four students the principles of Protein Electrophoresis. The kit contains the reagents to allow students to: Conduct a serial dilution of a stock amy..

Confirming the Amylase gene using PCR (Lab 13h)

This kit is designed to teach 8 groups of four students the principles of the polymerase chain reaction (PCR)   The kit contains the reagents to allow students to: Set up multiple PCR..

Determining the Concentration of Amylase (Lab 7G)

This kit is designed to teach 8 groups of four students the principles of protein concentration estimation assays. The kit contains the reagents to allow students to: Conduct a serial dilution o..

Direct ELISA of Bacterial alpha Amylase (Lab 6D)

This kit is designed to teach 8 groups of four students the principles of ELISA (Enzyme Linked ImmunoSorbent Assays).   The kit contains the reagents to allow students to: Conduct a s..

Restriction Digestion to Verify the p-Amylase Plasmid (Lab 8b)

This kit is designed to teach 8 groups of four students the principles of restriction digestion and agarose electrophoresis. The kit contains the reagents to allow students to: Set up restrictio..

Transformation of E. coli with p-Amylase (Lab 8C)

This kit is designed to teach 8 groups of four students the principles of bacterial transformation.   The kit contains the reagents to allow students to: Generate E.coli comptent cell..

Using a Miniprep Kit for p-Amylase Plasmid Isolation (Lab 8G)

This kit is designed to teach 8 groups of four students the principles of plasmid DNA purification by minipreps.   The kit contains the reagents to allow students to: Collect p-Amylas..

Using Ion Exchange Chromatography to Separate Proteins (Lab 9C)

This kit is designed to teach 8 groups of four students the principles of Ion Exchange Chromatography and protein purification.  The kit contains the reagents to allow students to: Equilibr..

Western Blot to Identify Alpha-Amylase (Lab 6E)

This kit is designed to teach 8 groups of four students the principles of Western blotting.   The kit contains the reagents to allow students to: Conduct a serial dilution of a stock ..