The rAmylase Project
The rAmylase Project is a unique set of ten self-contained laboratory experiments, kitted for teacher convenience. With The rAmylase Project Kits it is easy to pull together a unit, a semester, or a year-long course in biotechnology.
The kits can be used individually in a "plug'n'play" fashion to teach specific biotechnology techniques or, as a thematic unit that demonstrates how a commercially important biotechnology product, the amylase protein, is researched and manufactured. The rAmylase Project kits demonstrate that DNA is the flash but proteins are the cash of biotechnology.
The rAmylase Project Kits
- Characterizing Proteins by PAGE
- Assaying for Amylase Activity
- Direct ELISA of Bacterial α-Amylase
- Western Blot to Identify α-Amylase
- Determining the Concentration of Amylase in a Solution
- Restriction Digestion to Verify the pAmylase Plasmid
- Transformation of E. coli with pAmylase
- Using A Miniprep Kit for Plasmid Isolation
- Using Ion Exchange Chromatography to Separate Proteins
- Confirming the Amylase Gene Using PCR