Protein Electrophoresis

A major tool in the analysis of a species' proteome is the ability to separate and visualize the proteins by electrophoresis, 1D or 2D, and then the subsequent visualization of the proteins. Electrophoresis is a quick, simple and sensitive tool for separating proteins and nucleic acids by size and/or electrical charge. G-Biosciences offers a wide selection of electrophoresis kits, reagents and accessories. The G-Biosciences’ electrophoresis range extends from sample preparation for electrophoresis to running the protein samples by 1D and 2D electrophoresis.
 
G-Biosciences has also developed a wide selection of electrophoresis gel stains for visualizing proteins that vary in sensitivity, staining time and reversibility.
 
A wide selection of buffers and chemicals for gel casting and electrophoresis are also available.

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2D-Detergent™ Nonidet® P-40 Substitute

Many commercial grade detergents contain elevated levels of sulfhydryl oxidizing agents, peroxides, salts and carbonyl compounds (Figure 1). The proteins that are isolated with these detergents are hi..

2D-Detergent™ Triton® X-100

Many commercial grade detergents contain elevated levels of sulfhydryl oxidizing agents, peroxides, salts and carbonyl compounds (Figure 1). The proteins that are isolated with these detergents are hi..

2D-Xtract™

A suitable protein solubilization buffer for 2D analysis must solubilize proteins effectively, without disturbing the native charge of the proteins.  Urea based solubilization buffers solubilize ..

Acrylamide

Features CAS #: 79-06-1 Chemical Formula: C3H5NO Molecular Weight: 71.08   ..

Acrylamide/ Bisacrylamide (29:1), 40% Solution

Features 40% solution containing acrylamide (38.67%) and bisacrylamide (1.33%) for cross-linker ratio of 29:1 Acrylamide CAS #: 79-06-1 Acrylamide synonym: 2-Propenamide Acrylamide..

Agarose Sealing Solution

Simply heat and use for sealing the IPG strips for SDS-PAGE analysis. The Agarose Sealing Solution is prepared in a proprietary buffer to minimize reoxidation of the competing thiol pairs as proteins ..

Ammonium Acetate

Features CAS#: 631-61-8 Molecular Formula: C2H3O2NH4 Molecular Weight: 77.08 ..

Blue-OUT™

Blue-OUT™ is a unique product that washes the interfering Coomassie and fluorescent protein stains from gel pieces prior to their treatment for protein sequencing or mass spectrometry.   ..

Colloidal Blue Stain

G-Biosciences Colloidal Blue Stain is a mass spectrometry compatible stain that offers nanogram sensitive detection of proteins.  The stain offers ten times more sensitivity compared to classical..

Equilibration Buffer for IPG Strips

Add an appropriate volume of the supplied rehydration buffer to the dry, ready-to-use, urea based equilibration buffer.   Eliminates worries about urea induced protein carbamylation or waste of..

FOCUS™ FASTsilver™

FOCUS™ FASTsilver™ produces crystal clear backgrounds and maximal peptide recovery needed for critical analysis by mass spectrometry.   For mass spectrometry analysis, complete prot..

FOCUS™ Protein Alkylation

The kit is supplied with a proprietary buffer necessary for efficient alkylation of thiols, while minimizing reoxidation of the competing thiol pairs in protein samples. Simply add an appropriate amou..