Protein Electrophoresis

A major tool in the analysis of a species' proteome is the ability to separate and visualize the proteins by electrophoresis, 1D or 2D, and then the subsequent visualization of the proteins. Electrophoresis is a quick, simple and sensitive tool for separating proteins and nucleic acids by size and/or electrical charge. G-Biosciences offers a wide selection of electrophoresis kits, reagents and accessories. The G-Biosciences’ electrophoresis range extends from sample preparation for electrophoresis to running the protein samples by 1D and 2D electrophoresis.
 
G-Biosciences has also developed a wide selection of electrophoresis gel stains for visualizing proteins that vary in sensitivity, staining time and reversibility.
 
A wide selection of buffers and chemicals for gel casting and electrophoresis are also available.

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ß-Mercaptoethanol

High quality proteomic grade β-mercaptoethanol.   β-mercaptoethanol, a popular reducing agent, is offered in 5ml and 100ml bottles.Features Synonym: 2-Mercaptoethanol C..

2D-Detergent™ Nonidet® P-40 Substitute

Many commercial grade detergents contain elevated levels of sulfhydryl oxidizing agents, peroxides, salts and carbonyl compounds (Figure 1). The proteins that are isolated with these detergents are hi..

2D-Detergent™ Triton® X-100

Many commercial grade detergents contain elevated levels of sulfhydryl oxidizing agents, peroxides, salts and carbonyl compounds (Figure 1). The proteins that are isolated with these detergents are hi..

2D-Xtract™

A suitable protein solubilization buffer for 2D analysis must solubilize proteins effectively, without disturbing the native charge of the proteins.  Urea based solubilization buffers solubilize ..

Acrylamide

Features CAS #: 79-06-1 Chemical Formula: C3H5NO Molecular Weight: 71.08   ..

Acrylamide/ Bisacrylamide (29:1), 40% Solution

Features 40% solution containing acrylamide (38.67%) and bisacrylamide (1.33%) for cross-linker ratio of 29:1 Acrylamide CAS #: 79-06-1 Acrylamide synonym: 2-Propenamide Acrylamide..

Agarose Sealing Solution

Simply heat and use for sealing the IPG strips for SDS-PAGE analysis. The Agarose Sealing Solution is prepared in a proprietary buffer to minimize reoxidation of the competing thiol pairs as proteins ..

Ammonium Acetate

Features CAS#: 631-61-8 Molecular Formula: C2H3O2NH4 Molecular Weight: 77.08 ..

Blue-OUT™

Blue-OUT™ is a unique product that washes the interfering Coomassie and fluorescent protein stains from gel pieces prior to their treatment for protein sequencing or mass spectrometry.   ..

Colloidal Blue Stain

G-Biosciences Colloidal Blue Stain is a mass spectrometry compatible stain that offers nanogram sensitive detection of proteins.  The stain offers ten times more sensitivity compared to classical..

Coomassie Brilliant Blue

Coomassie Brilliant Blue R-250/ G-250 are both offered as dry powder form and convenient ready-to-use solutions. Destaining solutions are also offered.Features Synonym: Acid Blue 90 CAS#: 61..

Equilibration Buffer for IPG Strips

Add an appropriate volume of the supplied rehydration buffer to the dry, ready-to-use, urea based equilibration buffer.   Eliminates worries about urea induced protein carbamylation or waste of..