Cross-Linking & Modification

 Cross-linking agents contain at least two reactive groups that are reactive towards numerous groups, including sulfhydryls, amines and carbohydrates, and create chemical covalent bonds between two or more molecules.  functional groups that can be targeted with cross-linking agents are primary amines, carboxyls, sulfhydryls,  carbohydrates and carboxylic acids. Protein molecules have many of these functional groups and therefore proteins and peptides can be readily conjugated using cross-linking agents. Cross-linking agents are used to study protein structure and function, to anchor proteins to solid  supports, preparation of immunogens, immunotoxins, and other conjugated protein reagents. G-Biosciences offers a variety of Double Do™ cross-linking agents, reaction specific buffers, and accessories for performing and facilitating crosslinking applications. These accessory tools simplify cross-linking reactions and will enable researchers to achieve highly efficient cross-linking reactions with minimal effort in reaction optimization, set-up, and procedure development

We offer a variety of amino acid side chain modifiers to block side chain interactions, change the charge or modify them to be more favorable for other reactions. Many denaturants, or chaotropes, are available and can disrupt water interactions resulting in the solubilization of hydrophobic proteins and peptides. Chaotropes act on proteins to unfold them and alter their three-dimensional structure. A wide selection of reagents and accessories are also available.
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N-5-Azido-2-nitrobenzyloxysuccinimide    Features Molecular Weight: 305.20 Spacer Arm (Å): 7.7 Reactive Toward: Primary Amines Membran..


APDP A sulfhydryl-reactive and photoreactive cross-linker    Chemical Name  N-[4-(p-Azidosalicylamido)butyl]-3’-(2’-pyridyldith..


Bis(β-[4-azidosalicylamido]-ethyl) disulfide   Iodinatable  Features Molecular Weight: 474.52 Spacer Arm (Å): 21.3 Reactive Toward: Non S..

Bolton-Hunter Reagent (SHPP)

G-Biosciences Bolton-Hunter Reagent conjugates  tyrosine-like groups to end-terminal α-amino groups or ε-amino groups of lysine to increase the number of tyrosyl groups that ca..


(Bis(2-[Succinimidooxycarbonyloxy]ethyl) sulfoneFeatures Molecular Weight: 436.35 Spacer Arm (Å): 13 Reactive Toward: Primary Amines Membrane Permeabl..

Citraconic Anhydride

Citraconic anhydride reacts with primary amines and blocks them by creating an amide linkage and a terminal carboxylate (Figure 1).   The linkage is stable at neutral to alkaline pH (pH >7) ..


Anhydrous DMF [N,N-Dimethylformamide (HCON(CH3)2)]. Suitable for cross-linking, protein modification and biotinylation reaction applications...


1,4-Di-(3’-[2’pyridyldithio]-propionamido) butaneFeatures Molecular Weight: 428.71 Spacer Arm (Å): 19.9 Reactive Toward: Sulfhydryls Membr..


Dithiobis(succinimidyl propionate)Features Molecular Weight: 404.42 Spacer Arm (Å): 12 Reactive Toward: Primary Amines Membrane Permeable: YES Wa..


Disuccinimidyl suberate   Ideal for receptor ligand cross-linking and coupling antibody to Protein A or G for immunoprecipitations.   OneQuant™ DSS is supplied as 8 vials of 2mg DS..


Disuccinimidyl tartrateFeatures Molecular Weight: 344.24 Spacer Arm (Å): 6.4 Reactive Toward: Primary Amines Membrane Permeable: YES Water Solub..


3,3’-Dithiobis(sulfosuccinimidyl propionate)Features Molecular Weight: 608.51 Spacer Arm (Å): 12 Reactive Toward: Primary Amines Membrane Permea..